Impact of Oxidative Stress and Neuroinflammation on Sarco/Endoplasmic Reticulum Ca2+-ATPase 2b Downregulation and Endoplasmic Reticulum Stress in Temporal Lobe Epilepsy DOI Creative Commons
Vikas Yadav,

Sudipta Nayak,

Sandeep Guin

и другие.

ACS Pharmacology & Translational Science, Год журнала: 2024, Номер 8(1), С. 173 - 188

Опубликована: Дек. 16, 2024

Epilepsy is one of the most common neurological disorders. Calcium dysregulation and neuroinflammation are essential mechanisms in epileptogenesis. Sarco/endoplasmic reticulum (ER) Ca2+-ATPase 2b (SERCA2b), a crucial calcium regulatory pump, plays pathological roles various dysregulation-related diseases. However, link between SERCA2b epilepsy remains undetermined. This study aimed to establish relationship SERCA2b, oxidative stress, elucidate underlying molecular mechanism Neuroinflammation stress were induced N2a cells using lipopolysaccharide (LPS) hydrogen peroxide (H2O2). experimental temporal lobe (TLE) was mice pilocarpine. Further, effects on ER markers assessed at protein mRNA levels. imaging employed determine intracellular expression significantly decreased after LPS, H2O2, pilocarpine exposure both levels, mediated by upregulating neuroinflammation. downregulation associated with increased production reactive oxygen species elevated leading markers. Our findings highlight TLE. The results suggest that targeting could restore homeostasis processes, potentially providing therapeutic option for underscores importance pathophysiology its potential as target.

Язык: Английский

Protective Effect of Fisetin on Subacute and Chronic Arsenic and Fluoride Co‐Exposure Induced Hepatic, Renal and Cardiac Toxicities in Mice DOI Open Access

Sudipta Nayak,

Debarati Rakshit,

Vikas Yadav

и другие.

Journal of Biochemical and Molecular Toxicology, Год журнала: 2025, Номер 39(2)

Опубликована: Янв. 26, 2025

ABSTRACT Arsenic and fluoride are potent toxicants frequently present in drinking water food, posing severe health risks through toxic impacts on key organs, primarily by inducing oxidative stress. Combined exposure to these elements exacerbates damage, yet effective strategies for preventing managing their toxicities remain elusive. Fisetin, a bioflavonoid known its antioxidant anti‐inflammatory properties, has demonstrated protective effects multiple toxicity studies. However, effect arsenic‐ fluoride‐induced vital organs (particularly the liver, kidneys, heart) remains unexplored. This study investigates potential of fisetin against subacute chronic co‐exposure arsenic organ murine model. Male mice were administered water, while (5, 10, 20 mg/kg/day, orally) was co‐administered simultaneously. Animals sacrificed at 4 weeks (subacute) 24 (chronic) kidney, heart tissue analysis, assessing biochemical markers accumulation. Spectrophotometry revealed some degree chelation with fisetin. The results In Vivo studies showed that both led significant increases pro‐oxidant levels (ROS, TBARS, nitrite) accumulation, along reduced GSH content compared control. Fisetin treatment dose‐dependently stress exposed animals. findings highlight fisetin's activity capacity reduce burden as mechanisms. These support or fisetin‐rich fruit consumption therapeutic strategy mitigate from co‐exposure.

Язык: Английский

Процитировано

0

Development of eugenol fortified fisetin loaded nano-invasomes gel DOI
Abdul Ahad, Mohammad Raish,

Yousef A. BinJardan

и другие.

Xenobiotica, Год журнала: 2025, Номер unknown, С. 1 - 13

Опубликована: Март 13, 2025

1. The goal of current investigation was to develop eugenol fortified fisetin nano-invasomes. Fisetin loaded invasomes were prepared using thin film hydration procedure and evaluated for various parameters. Additionally, the optimized formulation (F5) converted gel Carbopol® as gelling agent pH, spreadability, homogeneity, drug content, in vitro release, antioxidant activity stability study.2. Prepared demonstrated vesicles size, PDI, zeta potential entrapment efficiency 153.85 ± 14.32 nm, 0.208 0.042, -12.67 1.08 mV 72.10 6.36%. TEM image indicated that are intact, spherical found range nanosized scale. showed better spreadability released contrast control gel. Substantial improvement DPPH radical scavenging 44.70% (3.1 µM) 83.94% (50 µM), noted compared at 39.47% 79.10% µM). stable 4 °C.3. Based on results, a viable method delivery bioactive compound(s) including fisetin.

Язык: Английский

Процитировано

0

Enhanced cognitive function in mice through intranasal delivery of sinapic acid via chitosan-coated solid lipid nanoparticles DOI

A Prabakaran,

Debarati Rakshit,

Inklisan Patel

и другие.

International Journal of Pharmaceutics, Год журнала: 2025, Номер 675, С. 125565 - 125565

Опубликована: Апрель 1, 2025

Язык: Английский

Процитировано

0

Bio‐Nano Innovations Targeting the Neurovascular Complex for Epilepsy Treatment DOI
Xin Wang, Yikang Cheng, Zhenghan Qi

и другие.

Advanced Healthcare Materials, Год журнала: 2025, Номер unknown

Опубликована: Апрель 30, 2025

Abstract Epilepsy is a prevalent chronic neurological disorder characterized by seizures resulting from an imbalance between excitatory and inhibitory neurons. While pharmacotherapy remains the standard treatment, traditional faces significant challenges, including poor brain penetration, high drug resistance rates, providing only symptomatic relief, rather than addressing underlying causes for comprehensive cure. Recently, neurovascular complex (NVC) has gained attention its critical role in development progression of epilepsy. Simultaneously, various innovative bio‐nanotechnology systems have emerged, specifically designed to enhance delivery across enable precise targeting within lesion. Herein, this review begins outlining core NVC involved epilepsy breaking it down into four key components: blood‐brain barrier (BBB), neurons, glial cells, microenvironment. The viability improve therapy analyzed. Next, systems, detailing their design principles, construction strategies, preclinical evaluations are highlighted. Finally, prospects next‐generation nanotechnologies challenges that must be overcome effective clinical translation discussed. Overall, aims guide more efficient bio‐nano therapies, ultimately enhancing treatment outcomes patients.

Язык: Английский

Процитировано

0

Nanoformulated fisetin ameliorates Alzheimer's disease via reducing proinflammatory cytokines and activating the NRF2/HO-1 pathway DOI

Debarati Rakshit,

Rajesh Goyal, Vikas Yadav

и другие.

Nanomedicine, Год журнала: 2024, Номер unknown, С. 1 - 17

Опубликована: Ноя. 18, 2024

The study aimed to evaluate the neuroprotective effect of a chitosan-coated fisetin nanoformulation in an experimental Alzheimer's disease (AD) model, focusing on improving fisetin's pharmacokinetics and exploring its impact both brain colon pathology.

Язык: Английский

Процитировано

2

Impact of Oxidative Stress and Neuroinflammation on Sarco/Endoplasmic Reticulum Ca2+-ATPase 2b Downregulation and Endoplasmic Reticulum Stress in Temporal Lobe Epilepsy DOI Creative Commons
Vikas Yadav,

Sudipta Nayak,

Sandeep Guin

и другие.

ACS Pharmacology & Translational Science, Год журнала: 2024, Номер 8(1), С. 173 - 188

Опубликована: Дек. 16, 2024

Epilepsy is one of the most common neurological disorders. Calcium dysregulation and neuroinflammation are essential mechanisms in epileptogenesis. Sarco/endoplasmic reticulum (ER) Ca2+-ATPase 2b (SERCA2b), a crucial calcium regulatory pump, plays pathological roles various dysregulation-related diseases. However, link between SERCA2b epilepsy remains undetermined. This study aimed to establish relationship SERCA2b, oxidative stress, elucidate underlying molecular mechanism Neuroinflammation stress were induced N2a cells using lipopolysaccharide (LPS) hydrogen peroxide (H2O2). experimental temporal lobe (TLE) was mice pilocarpine. Further, effects on ER markers assessed at protein mRNA levels. imaging employed determine intracellular expression significantly decreased after LPS, H2O2, pilocarpine exposure both levels, mediated by upregulating neuroinflammation. downregulation associated with increased production reactive oxygen species elevated leading markers. Our findings highlight TLE. The results suggest that targeting could restore homeostasis processes, potentially providing therapeutic option for underscores importance pathophysiology its potential as target.

Язык: Английский

Процитировано

0