Sensors and Actuators B Chemical, Год журнала: 2024, Номер unknown, С. 137145 - 137145
Опубликована: Дек. 1, 2024
Язык: Английский
Angewandte Chemie International Edition, Год журнала: 2024, Номер 63(41)
Опубликована: Июль 22, 2024
Abstract Self‐assembled supramolecular DNA tetrahedra composed of programmed sequence‐engineered complementary base‐paired strands represent elusive nanostructures having key contributions to the development and diverse applications nanotechnology. By appropriate engineering strands, tuneable sizes chemical functionalities were designed. Programmed for integrated into structures including sequence‐specific recognition (aptamers), catalytic DNAzymes, nanoparticles, proteins, or fluorophore. The article presents a comprehensive review addressing methods assemble characterize nanostructures, framework are discussed. Topics being addressed include application structurally functionalized nanostructure assembly optical electrochemical sensing platforms intracellular imaging modules. In addition, triggered reconfiguration dynamic networks circuits emulating biological transformations introduced. Moreover, functionalization frameworks with nanoparticles provides building units devices crystallization nanoparticle superlattices. Finally, in field nanomedicine addressed. These tetrahedra‐assisted permeation nanocarriers cells imaging, controlled drug release, active chemodynamic/photodynamic treatment target tissues, regenerative medicine.
Язык: Английский
Процитировано
10
Biosensors and Bioelectronics, Год журнала: 2024, Номер 263, С. 116621 - 116621
Опубликована: Июль 31, 2024
Язык: Английский
Процитировано
4
Biotechnology Advances, Год журнала: 2024, Номер 76, С. 108436 - 108436
Опубликована: Авг. 28, 2024
Язык: Английский
Процитировано
4
Biosensors and Bioelectronics, Год журнала: 2024, Номер 267, С. 116818 - 116818
Опубликована: Сен. 28, 2024
Язык: Английский
Процитировано
4
Analytical Chemistry, Год журнала: 2025, Номер unknown
Опубликована: Янв. 15, 2025
Developing a DNA autocatalysis-oriented cascade circuit (AOCC) via reciprocal navigation of two enzyme-free hug-amplifiers might be desirable for constructing rapid, efficient, and sensitive assay-to-treat platform. In response to specific trigger (T), seven functional hairpins were designed execute three-branched assembly (TBA) three isotropic hybridization chain reaction (3HCR) events operating the AOCC. This was because new inducers reconstructed in TBA arms initiate 3HCR (TBA-to-3HCR) periodic T repeats resultantly reassembled tandem nicks polymeric nanowires rapidly activate opposite direction (3HCR-to-TBA) without steric hindrance, thereby cooperatively manipulating sustainable AOCC progress exponential hug-amplification (1:3Nn). Our experimental verifications manifested that T-dependent amplifier achieved fast input transduction efficient fluorescence readout. As predicted, flexible programming reactive hairpin species endowed repeating productive with great possibilities accessibilities graft tailored modular elements, such as G-rich AS1411 aptamers capable adopting G-quadruplex conformations (G4) readily facilitated embedding zinc(II) protoporphyrin IX (ZnPPIX), kind heme oxygenase-1 enzyme inhibitor. Thus, cascading ZnPPIX/G4 entities acted fluorescent signal reporters, photosensitizers anticancer drugs, creating an updated AOCC-based platform ultrasensitive biosensing, discernible cell imaging photodynamic therapy cancer cells. would offer paradigm advance rational integration dynamic amplifiable recycling circuits applicable bioassay theranostics.
Язык: Английский
Процитировано
0
LWT, Год журнала: 2025, Номер 218, С. 117475 - 117475
Опубликована: Фев. 1, 2025
Язык: Английский
Процитировано
0
Chemical & Biomedical Imaging, Год журнала: 2025, Номер unknown
Опубликована: Март 20, 2025
Язык: Английский
Процитировано
0
Analytical Chemistry, Год журнала: 2025, Номер unknown
Опубликована: Март 27, 2025
Fibroblast phenotypic transformation plays a crucial role in tumorigenesis. Tumor cell-derived exosome-mediated fibroblast has been commonly studied. However, there is still no direct evidence that the process of tumor exosomes induces fibroblasts into cancer-associated (CAFs). Herein, exosomal miRNAs were used as target to track invasion cancer normal fibroblasts, and FAP mRNA, CAF biomarker, further trace effect on recipient cells. Two DNA tetrahedral sensors developed based hybrid chain reaction signal amplification strategy fluorescence resonance energy transfer mechanism. Both showed high sensitivity good selectivity, with detection limits 4.94 6.61 pM for miR-1247-3p respectively. Moreover, two allowed us image living cell postinvasion tumor-derived exosomes, facilitating visual monitoring phenotype uncovering new perspectives metastasis development.
Язык: Английский
Процитировано
0
Angewandte Chemie, Год журнала: 2024, Номер 136(41)
Опубликована: Июль 22, 2024
Abstract Self‐assembled supramolecular DNA tetrahedra composed of programmed sequence‐engineered complementary base‐paired strands represent elusive nanostructures having key contributions to the development and diverse applications nanotechnology. By appropriate engineering strands, tuneable sizes chemical functionalities were designed. Programmed for integrated into structures including sequence‐specific recognition (aptamers), catalytic DNAzymes, nanoparticles, proteins, or fluorophore. The article presents a comprehensive review addressing methods assemble characterize nanostructures, framework are discussed. Topics being addressed include application structurally functionalized nanostructure assembly optical electrochemical sensing platforms intracellular imaging modules. In addition, triggered reconfiguration dynamic networks circuits emulating biological transformations introduced. Moreover, functionalization frameworks with nanoparticles provides building units devices crystallization nanoparticle superlattices. Finally, in field nanomedicine addressed. These tetrahedra‐assisted permeation nanocarriers cells imaging, controlled drug release, active chemodynamic/photodynamic treatment target tissues, regenerative medicine.
Язык: Английский
Процитировано
3
Advanced Functional Materials, Год журнала: 2024, Номер 34(44)
Опубликована: Авг. 18, 2024
Abstract DNA walker nanomachines are in fast development for biosensing, medical diagnostics, and food safety monitoring. For practical application, the issues of slow dynamics low operational efficiency hinder its widespread application. Inspired by high agricultural cooperative machinery, a unique cleaving‐rolling‐assembly integrated nanodevice (CRAIN) is constructed high‐performance detection various biomolecules. In design, nanotubes introduced as building loaders to engineer polyoriented walking nanorollers with blocked DNAzyme‐based strands thus increase reaction direction local concentration. Through target‐specific recognition, activated can autonomously progressively cleave roll along directional hairpin tracks on gold substrate, significantly accelerating rate. Moreover, using nucleic acid‐encoded urchin‐shaped multispike gold‐silver composite nanospheres (nMGSs) SERS‐enhanced probes, engineered assembly nMGSs probes induced cleaving‐rolling process promotes formation high‐density hotspot domains, further improving sensitivity. With rational CRAIN system be extended perform highly sensitive analyses analytes, such microRNA‐21, butyrylcholinesterase activity organophosphorus pesticides. This strategy provide new perspectives clinical diagnosis, biomedical engineering, environmental science.
Язык: Английский
Процитировано
3