Materials Today Bio, Год журнала: 2024, Номер 30, С. 101386 - 101386
Опубликована: Дек. 9, 2024
Язык: Английский
Chemistry - Methods, Год журнала: 2025, Номер unknown
Опубликована: Март 21, 2025
Abstract Block copolymer self‐assembly into nanoparticles of defined size and morphology is facilitated via continuous flow synthesis methods. Using flow, are obtained at higher rates, with improved consistency, under greener conditions significantly reduced batch‐to‐batch variability when compared to traditional batch processes. The methodology formulate block copolymers in a setup described, design strategies explained. Further, the purification particles dialysis marking second important step synthesis, which performed time resource intensive. described methods open pathway for reproducible nanoparticle towards automation high‐throughput screening materials.
Язык: Английский
Процитировано
0
Cell Communication and Signaling, Год журнала: 2025, Номер 23(1)
Опубликована: Апрель 1, 2025
Abstract Background Tuberculosis (TB) is a contagious disease and the second leading cause of death worldwide. The Bacille Calmette–Guérin (BCG) vaccine, only licensed TB has insufficient protective efficacy in adults, necessitating development new vaccines. Ag85B, protein-subunit promising candidate due to its high immunogenicity. However, hydrophobicity presents challenges manufacturing, expression, purification, Ag85B alone does not elicit sufficient immune stimulation. To overcome these limitations, this study aimed design temperature-responsive amine-terminated polylactic acid (PLA)-based nanosponge (aPNS) as both nanoadjuvant an efficient delivery carrier for Ag85B. Methods was produced using EZtag fusion tag vector, achieving product yield purity. It then loaded into aPNS, nanoparticle system with PLA core Pluronic shell, through process at 4 °C that preserved protein bioactivity. stability sustained-release profile Ag85B@aPNS were evaluated. In vitro cytotoxicity cellular uptake studies conducted macrophages. Protective immunogenicity assessed M. tuberculosis -challenged mice BCG-primed mice. Results successfully which exhibited good colloidal profile. Neither synthesized nor aPNS showed significant cytotoxicity. enhanced antigens by Compared BCG, demonstrated superior against improved Conclusion viable vaccination, showing potential standalone vaccine BCG-booster. Its ability enhance provide protection highlights promise addressing limitations current
Язык: Английский
Процитировано
0
Macromolecules, Год журнала: 2025, Номер unknown
Опубликована: Май 19, 2025
Язык: Английский
Процитировано
0
Expert Opinion on Drug Delivery, Год журнала: 2025, Номер unknown
Опубликована: Май 26, 2025
The nasal administration route has gained peak interest in recent literature and as a noninvasive alternative for efficient drug delivery increasing bioavailability of active substances. Technological challenges arise from the drug's physicochemical properties mucosal barrier which innovative particle engineering techniques must be implemented, such using polymeric nanocarriers. This review deals with importance its connection to micelles period between 2015 - 2025 up date was chosen search original research articles where were applied nasally. first part demonstrates utilization micelles, followed by summary how release permeability can achieved cavity through epithelium. second reviews studies conducted on this matter. could superior perform suitable conventional routes. Multiple have already demonstrated that main advantages lie nose-to-brain pathway, conquered via adequately formulated micelles. As an solution, vaccine is also great potential combining
Язык: Английский
Процитировано
0
Materials Today Bio, Год журнала: 2024, Номер 30, С. 101386 - 101386
Опубликована: Дек. 9, 2024
Язык: Английский
Процитировано
2