Neuroradiology,
Год журнала:
2023,
Номер
66(3), С. 305 - 315
Опубликована: Дек. 16, 2023
Abstract
Currently,
with
the
knowledge
of
role
collateral
circulation
in
development
cerebral
ischaemia,
traditional
therapeutic
windows
are
being
prolonged,
time
not
only
criterion.
Instead,
a
more
personalised
approach
is
applied
to
select
additional
patients
who
might
benefit
from
active
treatment.
This
review
briefly
describes
current
pathophysiology
early
ischaemic
changes,
capabilities
MRI
depict
such
and
basics
routinely
used
imaging
techniques
broadly
available
for
assessment
individual
phases
summarises
possible
clinical
use
routine
MR
imaging,
including
patient
selection
treatment
outcome
on
basis
imaging.
International Journal of Molecular Sciences,
Год журнала:
2023,
Номер
24(13), С. 10739 - 10739
Опубликована: Июнь 27, 2023
The
aim
of
this
review
is
to
present
evidence
the
impact
ischemic
changes
in
blood–brain
barrier
on
maturation
post-ischemic
brain
neurodegeneration
with
features
Alzheimer’s
disease.
Understanding
processes
involved
permeability
during
recirculation
will
provide
clinically
relevant
knowledge
regarding
neuropathological
that
ultimately
lead
dementia
disease
type.
In
review,
we
try
distinguish
between
primary
and
secondary
after
ischemia.
Therefore,
can
observe
two
hit
stages
contribute
development.
onset
pathology
includes
neuronal
damage
death
followed
by
injury
serum
leakage
amyloid
into
tissue,
leading
increased
susceptibility
neurotoxicity,
culminating
formation
plaques
ending
full-blown
Journal of Neuroinflammation,
Год журнала:
2023,
Номер
20(1)
Опубликована: Июль 29, 2023
Abstract
Background
Brain
microglia
and
macrophages
(Mi/MΦ)
can
shift
to
a
harmful
or
advantageous
phenotype
following
an
ischemic
stroke.
Identification
of
key
molecules
that
regulate
the
transformation
resting
Mi/MΦ
could
aid
in
development
innovative
therapies
for
The
transcription
factor
signal
transducer
activator
transduction
1
(STAT1)
has
been
found
contribute
acute
neuronal
death
(in
first
24
h)
stroke,
but
its
effects
on
influence
long-term
stroke
outcomes
have
yet
be
determined.
Methods
We
generated
mice
with
tamoxifen-induced,
Mi/MΦ-specific
knockout
(mKO)
STAT1
driven
by
Cx3cr1
CreER
.
Expression
was
examined
brain
flow
cytometry
RNA
sequencing
after
induced
transient
middle
cerebral
artery
occlusion
(MCAO).
impact
mKO
cell
death,
phenotype,
inflammation
profiles
were
3–5
days
MCAO.
Neurological
deficits
integrity
gray
white
matter
assessed
5
weeks
MCAO
various
neurobehavioral
tests
immunohistochemistry.
Results
activated
at
subacute
stage
(3
days)
Selective
deletion
did
not
alter
infarct
size
h
MCAO,
attenuated
release
high
mobility
group
box
increased
arginase
1-producing
3d
suggesting
boosted
inflammation-resolving
responses
Mi/MΦ.
As
result,
had
mitigated
less
injury
long
term.
Importantly,
sufficient
improve
functional
recovery
least
both
male
female
mice.
Conclusions
Mi/MΦ-targeted
KO
does
provide
immediate
neuroprotection
augments
actions
Mi/MΦ,
thereby
facilitating
is,
therefore,
promising
therapeutic
target
harness
beneficial
International Journal of Nanomedicine,
Год журнала:
2024,
Номер
Volume 19, С. 6177 - 6199
Опубликована: Июнь 1, 2024
Purpose:
Ginsenoside
Rg3
(Rg3)
and
Panax
notoginseng
saponins
(PNS)
can
be
used
for
ischemic
stroke
treatment,
however,
the
lack
of
targeting
to
region
limits
therapeutic
effect.
To
address
this,
we
leveraged
affinity
macrophage
membrane
proteins
inflamed
brain
microvascular
endothelial
cells
develop
a
membrane-cloaked
liposome
loaded
with
PNS
(MM-Lip-Rg3/PNS),
which
precisely
target
lesion
through
intranasal
administration.
Methods:
MM-Lip-Rg3/PNS
was
prepared
by
co-extrusion
method
performed
characterization,
stability,
surface
protein,
morphology.
The
cellular
uptake,
immune
escape
ability,
blood-brain
barrier
crossing
ability
were
studied
in
vitro.
vivo
targeting,
biodistribution
anti-ischemic
efficacy
evaluated
MACO
rats,
determined
diversity
nasal
pathway
olfactory
nerve
blockade
model
rats.
Finally,
pharmacokinetics
index
investigated.
Results:
Our
results
indicated
that
spherical
shell-core
structure.
avoid
mononuclear
phagocytosis,
actively
bind
inflammatory
cells,
have
cross
barrier.
Moreover,
could
specifically
sites,
even
microglia,
increase
cumulative
number
drugs
brain,
improve
environment
reduce
infarct
size.
By
comparing
nerve-blocking
rats
normal
it
found
there
are
direct
indirect
pathways
entry
into
brain.
Pharmacokinetics
demonstrated
exhibited
stronger
prolonged
drug
half-life.
Conclusion:
might
contribute
accumulation
area
treatment
efficacy.
This
biomimetic
nano-drug
delivery
system
provides
new
promising
strategy
stroke.
Keywords:
macrophage-membrane
coating,
liposome,
stroke,
Fitoterapia,
Год журнала:
2024,
Номер
177, С. 106098 - 106098
Опубликована: Июнь 29, 2024
Brain
edema
after
ischemic
stroke
could
worsen
cerebral
injury
in
patients
who
received
intravenous
thrombolysis.
Cornus
officinalis
Sieb.
et
Zucc.,
a
long-established
traditional
Chinese
medicine,
is
beneficial
to
the
treatment
of
neurodegenerative
diseases
including
stroke.
In
particular,
its
major
component,
cornel
iridoid
glycoside
(CIG),
was
evidenced
exhibit
neuroprotective
effects
against
ischemic/reperfusion
(CIR/I).
Aimed
explore
CIG
on
brain
CIR/I
rats,
analyzed
with
main
constituents
by
using
HPLC.
The
molecular
docking
analysis
performed
between
and
AQP4-M23.
TGN-020,
an
AQP4
inhibitor,
used
as
comparison.
vivo
experiments,
rats
were
pre-treated
injured
performing
middle
artery
occlusion/reperfusion
(MCAO/R).
After
24
h,
examined
for
neurological
function,
pathological
changes,
edema,
polarized
Aqp4
expressions
brain.
HPLC
indicated
that
composed
morroniside
loganin.
showed
both
loganin
displayed
lower
binding
energies
AQP4-M23
than
TGN-020.
exhibited
fewer
function
deficits,
minimized
swelling,
reduced
lesion
volumes
compared
MCAO/R
rats.
peri-infarct
infarct
regions,
pre-treatment
restored
expression
which
lost
results
suggested
attenuate
ischemia/reperfusion
modulating
through
interaction
Neural Regeneration Research,
Год журнала:
2024,
Номер
20(5), С. 1221 - 1235
Опубликована: Май 10, 2024
Current
therapeutic
strategies
for
ischemic
stroke
fall
short
of
the
desired
objective
neurological
functional
recovery.
Therefore,
there
is
an
urgent
need
to
develop
new
methods
treatment
this
condition.
Exosomes
are
natural
cell-derived
vesicles
that
mediate
signal
transduction
between
cells
under
physiological
and
pathological
conditions.
They
have
low
immunogenicity,
good
stability,
high
delivery
efficiency,
ability
cross
blood-brain
barrier.
These
properties
exosomes
potential
lead
breakthroughs
in
stroke.
The
rapid
development
nanotechnology
has
advanced
application
engineered
exosomes,
which
can
effectively
improve
targeting
ability,
enhance
efficacy,
minimize
dosages
needed.
Advances
technology
also
driven
clinical
translational
research
on
exosomes.
In
review,
we
describe
effects
their
positive
roles
current
stroke,
including
anti-inflammation,
anti-apoptosis,
autophagy-regulation,
angiogenesis,
neurogenesis,
glial
scar
formation
reduction
effects.
However,
it
worth
noting
that,
despite
significant
potential,
remains
a
dearth
standardized
characterization
efficient
isolation
techniques
capable
producing
highly
purified
Future
optimization
should
prioritize
exploration
suitable
establishment
unified
workflows
harness
diagnostic
or
applications
Ultimately,
our
review
aims
summarize
understanding
exosome-based
prospects
foster
innovative
ideas
therapies.
Brain Research Bulletin,
Год журнала:
2025,
Номер
unknown, С. 111293 - 111293
Опубликована: Март 1, 2025
Cerebral
ischemia-reperfusion
injury
(CIRI)
is
a
leading
cause
of
neurological
impairment
in
stroke,
primarily
correlated
to
oxidative
stress,
inflammation,
and
ferroptosis.
This
study
investigates
the
neuroprotective
effects
hirudin
on
CIRI,
focusing
its
role
modulating
neuronal
survival,
ferroptosis
markers
through
inhibition
CCL2.
A
middle
cerebral
artery
occlusion
(MCAO)
model
mice
an
oxygen-glucose
deprivation/reoxygenation
(OGD/R)
HT22
cells
were
used
simulate
ischemic
conditions.
Hirudin
significantly
improved
function
reduced
edema
infarct
size
MCAO
model.
In
vitro,
enhanced
viability
apoptosis
OGD/R-stimulated
cells.
Integrative
network
pharmacology
transcriptomic
analysis
identified
CCL2
as
potential
target
hirudin.
treatment
suppressed
expression,
which
turn
TLR4/NF-κB
signaling
activation,
thereby
mitigating
inflammatory
responses
neurons.
Overexpression
partially
reversed
these
protective
effects,
underscoring
injury.
These
findings
suggest
that
alleviates
CIRI
by
preventing
ferroptosis,
offering
insights
into
therapeutic
agent
for
Stroke and Vascular Neurology,
Год журнала:
2025,
Номер
unknown, С. svn - 003675
Опубликована: Март 16, 2025
Background
The
increased
permeability
of
the
blood-brain
barrier
(BBB)
is
a
critical
contributor
to
high
mortality
following
ischaemic
stroke.
However,
mechanisms
regulating
BBB
integrity
remain
poorly
understood.
Leucine-rich
repeat-containing
8A
(LRRC8A)
chloride
channel
for
cellular
volume
homeostasis
and
plays
key
role
in
neuronal
injury
during
ischaemia.
its
impact
on
function
currently
unclear.
Methods
A
transient
middle
cerebral
artery
occlusion
model
was
established
investigate
LRRC8A
integrity.
Laser
speckle
contrast
imaging
used
monitor
cortical
blood
flow.
Primary
mouse
human
brain
microvascular
endothelial
cells
(m/hBMVECs)
were
subjected
oxygen-glucose
deprivation
(OGD)
re-oxygenation
varying
durations.
Patch-clamp
recordings
performed
measure
volume-regulated
currents.
Immunostaining
conducted
evaluate
protein
expression.
Cell
evaluated
with
transwell
assay.
Results
deletion
ameliorates
infarct
area
mitigates
leakage.
Ischaemia
dramatically
upregulates
expression
cells,
concurrently
downregulating
tight
junction
proteins.
OGD
exposure
augments
VRCC
current
mediated
by
BMVECs.
In
contrast,
inhibiting
promotes
ZO-1
VE-cadherin,
thereby
preserving
cells.
With-no-lysine
kinase
1
(WNK1)
inhibition
contributes
LRRC8A-induced
damage
post-ischaemic
Eupatorin,
newly
identified
inhibitor,
exerts
neuroprotective
effects
against
Conclusions
BMVECs
pivotal
modulating
integrity,
process
regulated
WNK1.
As
an
Eupatorin
holds
potential
stroke
therapy.
