Synaptic Targets of Circadian Clock Neurons Influence Core Clock Parameters DOI Creative Commons
Eva Scholz-Carlson, Aishwarya Ramakrishnan, Aljoscha Nern

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown

Опубликована: Янв. 31, 2025

Neuronal connectivity in the circadian clock network is essential for robust endogenous timekeeping. In Drosophila network, four pairs of small ventral lateral neurons (sLNvs) serve as main pacemakers. Peptidergic communication via sLNv, which release key output neuropeptide, Pigment Dispersing Factor (PDF), has been well characterized. absence PDF, flies become largely arrhythmic, similar to phenotype associated with loss mammalian peptide, VIP. contrast, little known about role synaptic connections that sLNvs form downstream neurons. Connectomic analyses revealed despite their pacemaker within very few monosynaptic other However, they strong a group previously uncharacterized neurons, SLP316, turn synapse onto dorsal Here, we show silencing SLP316 tetanus toxin (TNT) expression shortens free-running period, whereas hyper-exciting them by expressing constitutively open Na[+] channel, NaChBac, results period lengthening. Under light-dark cycles, also causes lower daytime activity and higher sleep, likely due increased sleep pressure. Our postsynaptic partners are non-clock neuronal cell type regulates timing activity.

Язык: Английский

Synaptic Targets of Circadian Clock Neurons Influence Core Clock Parameters DOI Creative Commons
Eva Scholz-Carlson, Aishwarya Ramakrishnan, Aljoscha Nern

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown

Опубликована: Янв. 31, 2025

Neuronal connectivity in the circadian clock network is essential for robust endogenous timekeeping. In Drosophila network, four pairs of small ventral lateral neurons (sLNvs) serve as main pacemakers. Peptidergic communication via sLNv, which release key output neuropeptide, Pigment Dispersing Factor (PDF), has been well characterized. absence PDF, flies become largely arrhythmic, similar to phenotype associated with loss mammalian peptide, VIP. contrast, little known about role synaptic connections that sLNvs form downstream neurons. Connectomic analyses revealed despite their pacemaker within very few monosynaptic other However, they strong a group previously uncharacterized neurons, SLP316, turn synapse onto dorsal Here, we show silencing SLP316 tetanus toxin (TNT) expression shortens free-running period, whereas hyper-exciting them by expressing constitutively open Na[+] channel, NaChBac, results period lengthening. Under light-dark cycles, also causes lower daytime activity and higher sleep, likely due increased sleep pressure. Our postsynaptic partners are non-clock neuronal cell type regulates timing activity.

Язык: Английский

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