Physiology & Behavior, Год журнала: 2024, Номер unknown, С. 114788 - 114788
Опубликована: Дек. 1, 2024
Язык: Английский
Physiological Reports, Год журнала: 2025, Номер 13(2)
Опубликована: Янв. 1, 2025
Abstract The effects of social isolation (SI) during middle age remain unclear, so we tested the hypothesis that SI would lead to an increase in impulsive choice (IC), anxiety‐like behavior, and metabolic dysfunction middle‐aged rats. Male female rats were housed individually or groups four with same‐sex housing mates at 11 months age. Two later, IC behavior was assessed using a delay‐discounting task through novelty‐suppressed feeding (NSF) task. Lastly, glucose tolerance insulin sensitivity following exposure high‐fat diet oral test (OGTT) (ITT). results showed socially isolated displayed more than did group‐housed both sexes. However, no significant effect evident NSF task, OGTT, ITT. had higher plasma concentration resistance index compared females. Our findings demonstrate is sufficient highlight inherent sex‐specific differences profiles. These underscore importance investigating mechanisms underlie different stages life.
Язык: Английский
Процитировано
0
Frontiers in Psychiatry, Год журнала: 2025, Номер 15
Опубликована: Фев. 6, 2025
Anxiety disorders are prevalent mental health conditions characterized by significant impairments in daily functioning and social interactions. Despite the effectiveness of pharmacological treatments, challenges such as medication resistance, adverse side effects, high rate relapse necessitate exploration alternative therapies. Recently, electroacupuncture (EA) has garnered attention a promising non-pharmacological intervention for anxiety disorders; however, mechanisms which EA exerts its anxiolytic effects remain poorly understood. This study aims to elucidate role microglial cells anxiety, specifically examining how modulates morphology function within basolateral amygdala (BLA) context induced isolation. Utilizing mouse model isolation-induced we evaluated anxiety-like behaviors through Elevated Plus Maze (EPM) Open Field Test (OFT). Additionally, biochemical analyses immunofluorescence imaging were performed assess expression NADPH oxidase 2 (NOX2), activation markers, levels oxidative stress. Our findings reveal that treatment significantly mitigates mice, correlating with reduction NOX2 BLA microglia decreased reactive oxygen species (ROS). Furthermore, was observed restore normal morphology, indicating potential modulating activity. The results this suggest modulation stress activity BLA. These provide new insights into cellular underlying therapeutic EA, highlighting strategies management paving way future research aimed at improving clinical outcomes individuals suffering from anxiety.
Язык: Английский
Процитировано
0
NeuroImage, Год журнала: 2025, Номер unknown, С. 121117 - 121117
Опубликована: Март 1, 2025
During early life stages, social isolation disrupts the proper brain growth and circuit formation, which is associated with risk of mental disorders cognitive deficits in adulthood. Nevertheless, impact juvenile on development, particularly regarding variations across age sex, remains poorly understood. Here, we investigate effects stress (SIS) during (3-5 weeks old) or late (5-7 period morphology adult male female mice using ultra high-field MRI (11.7 T). We found that both SIS led to volumetric increases multiple regions, such as medial prefrontal cortex (mPFC) hippocampus. Correlation tractography revealed fiber tracts right corpus callosum amygdala were positively correlated mice. In mice, resulted small isocortex, whereas reductions isocortex hypothalamus. Furthermore, caused a negative correlation, while exhibited positive Using Random Forest classifier, achieved effective discrimination between socially isolated control conditions volume limbic areas playing key role model's accuracy. Finally, discovered context fear generalization sex-dependent manner. Our findings highlight importance considering time- development emotional processing, providing new insights into its long-term consequences.
Язык: Английский
Процитировано
0
European journal of medical research, Год журнала: 2025, Номер 30(1)
Опубликована: Март 20, 2025
Neuropathic pain is characterized by hyperalgesia, allodynia, and inflammation it often resistant to treatment. The formyl peptide receptor 2 (ALX/FPR2), a G-protein-coupled receptor, has been implicated in resolving inflammation, making its agonist, Resolvin D1 (RvD1), potential therapeutic agent. Previous studies suggest that RvD1 alleviates neuropathic via anti-inflammatory effects, but mechanisms remain unclear, particularly relation microglial activation the brain-derived neurotrophic factor (BDNF)/TrkB signaling pathway. To investigate analgesic effects of spared nerve injury (SNI) model explore through regulation neuroinflammation BDNF/TrkB SNI mice received intrathecal at varying doses (10–40 ng) determine efficacy reducing mechanical allodynia thermal sensitivity. were assessed using ELISA, immunofluorescence, western blotting measure expression pro-inflammatory cytokines BDNF. involvement ALX/FPR2 TrkB receptors was further examined antagonists Boc2 K252a. significantly reduced dose-dependent manner. also decreased (TNF-α, IL-1β, IL-6) BDNF both vivo vitro models. These reversed K252a, confirming actions are mediated inhibition signaling. suppressing findings as promising agent for management.
Язык: Английский
Процитировано
0
bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown
Опубликована: Июнь 25, 2024
Stress is a major risk for the onset of several maladaptive processes including pathological anxiety, diffuse state heightened apprehension over anticipated threats
Язык: Английский
Процитировано
0
bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown
Опубликована: Июль 13, 2024
Abstract Adolescence is a critical period for brain development in humans and stress exposure during this time can have lasting effects on behavior development. Social isolation loneliness are particularly salient stressors that lead to detrimental mental health outcomes females, although most of the preclinical work social has been done male animals. Our lab developed model post-weaning adolescent leads increased drug reward sensitivity altered neuronal structure limbic regions. The current study utilized determine impact three-chamber interaction task both adolescence adulthood. We found while does not alter (PND45), it sex-specific adulthood (PND60), potentiating mice decreasing female mice. As early life activate microglia leading alterations pruning, we next examined inhibiting microglial activation with daily minocycline administration first three weeks these changes interaction. During adolescence, dampened mice, having no effect females. In contrast, adulthood, did males, socially isolated males exhibiting higher levels compared their group housed counterparts. treatment reversed group, mimicking what seen naïve males. Taken together, alters but
Язык: Английский
Процитировано
0
Frontiers in Behavioral Neuroscience, Год журнала: 2024, Номер 18
Опубликована: Авг. 29, 2024
Adolescence is a critical period for brain development in humans and stress exposure during this time can have lasting effects on behavior development. Social isolation loneliness are particularly salient stressors that lead to detrimental mental health outcomes females, although most of the preclinical work social has been done male animals. Our lab developed model post-weaning adolescent leads increased drug reward sensitivity altered neuronal structure limbic regions. The current study utilized determine impact three-chamber interaction task both adolescence adulthood. We found while does not alter (PND45), it sex-specific young adulthood (PND60), potentiating mice decreasing female mice. As early life activate microglia leading alterations pruning, we next examined inhibiting microglial activation with daily minocycline administration first 3 weeks these changes interaction. During adolescence, dampened mice, having no effect females. In contrast, adulthood, did males, socially isolated males exhibiting higher levels compared their group housed counterparts. treatment reversed group, mimicking what seen naïve males. Taken together, alters but
Язык: Английский
Процитировано
0
Environment & Health, Год журнала: 2024, Номер unknown
Опубликована: Ноя. 19, 2024
Environmental enrichment (EE) is a significant approach to influencing brain function by altering the environment and changing living conditions has been shown modulate mood-related diseases, including depression. Among elderly, depression particularly prevalent often linked social isolation. However, specific role of EE in isolation-related geriatric remains imprecise. This study was intended explore status exposure uncover its underlying mechanisms. We utilized 19-month-old male C57BL/6J mice, which are equivalent humans aged 50–60 years, induced through After 2 weeks isolation, mice were identified as depressive using sugar preference test then classified into either standard or groups for 4 weeks. Subsequently, conventional indices associated with depression, neurogenesis, neurotrophic factors, neuroinflammation, measured. Results display that alleviated depressive-like symptoms elderly enriched their activities. Concurrently, regulated levels certain neurotransmitters hippocampus, systems glutamate, tyrosine, histamine. Moreover, ability neurogenesis also increased hippocampus mice. At neuroinflammation level, activation Natural Killer (NK) cells ARG1+ microglia considered major contributor mediating effects EE-regulated Collectively, these results underline importance treatment partially elucidate mechanism, offering valuable suggestions treating isolation--related via environmental modulation.
Язык: Английский
Процитировано
0
Physiology & Behavior, Год журнала: 2024, Номер unknown, С. 114788 - 114788
Опубликована: Дек. 1, 2024
Язык: Английский
Процитировано
0