An anesthetic protocol for preserving functional network structure in the marmoset monkey brain

Imaging Neuroscience, Год журнала: 2024, Номер 2, С. 1 - 23

Опубликована: Янв. 1, 2024

Abstract Initiatives towards acquiring large-scale neuroimaging data in non-human primates promise improving translational neuroscience and cross-species comparisons. Crucial among these efforts is the need to expand sample sizes while reducing impact of anesthesia on functional properties brain networks. Yet, effects primate networks remain unclear. Here, we demonstrate using magnetic resonance imaging (fMRI) at 9.4 tesla that isoflurane induces a variety states marmoset with dramatically altered connectivity profiles. As an alternative, recommend continuous infusion sedative medetomidine, supplemented low concentration isoflurane. Using this protocol eight marmosets, observed robust visual activation during flickering light stimulation identified resting-state similar awake state. In contrast, alone led suppressed absence awake-like network patterns. Comparing graph-theoretical approach, confirmed structure preserved under our proposed but lost levels greater than 1%. We believe widespread adoption will be step advancing initiatives neuroimaging. To promote collaborative use resources, openly share datasets (https://zenodo.org/records/11118775).

Язык: Английский

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Dynamics of directional motor tuning in the primate premotor and primary motor cortices during sensorimotor learning

Nature Communications, Год журнала: 2024, Номер 15(1)

Опубликована: Авг. 20, 2024

Sensorimotor learning requires reorganization of neuronal activity in the premotor cortex (PM) and primary motor (M1). To reveal PM- M1-specific a primate, we conducted calcium imaging common marmosets while they learned two-target reaching (pull/push) task after mastering one-target (pull) task. Throughout task, dorsorostral PM (PMdr) showed peak earlier than dorsocaudal (PMdc) M1. During learning, reaction time pull trials increased correlated strongly with timing PMdr activity. decreasing representation newly introduced (push) movement, whereas PMdc M1 maintained high push movements. Many task-related neurons exhibited strong preference to either movement direction. dynamically switched their preferred direction depending on performance early stage, stably retained similarity between neighbors. These results suggest that primate sensorimotor dynamic directional tuning converts association formed stable specific

Язык: Английский

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Common marmoset: An emerging non-human primate model for translational applications in brain disorders

Current Opinion in Neurobiology, Год журнала: 2025, Номер 92, С. 102998 - 102998

Опубликована: Март 15, 2025

Язык: Английский

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Targeted gene transfer into developmentally defined cell populations of the primate brain

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown

Опубликована: Апрель 17, 2025

Abstract The primate brain possesses unique physiological and developmental features whose systematic investigation is hampered by a paucity of transgenic germline models tools. Here, we present minimally invasive method to introduce transgenes widely across the cerebral cortex using ultrasound-guided fetal intracerebroventricular viral injections (FIVI). This technique enables rapid-onset long-lasting transgene expression following delivery recombinant adeno-associated viruses (rAAVs). By adjusting gestational timing injections, serotypes, transcriptional regulatory elements, rAAV FIVI allows for targeting specific cell populations. We demonstrate versatility this through restricted laminar in cortex, Cre-dependent neurons, CRISPR-based gene editing, labeling peripheral somatosensory retinal pathways. mimicking key desirable models, efficient targeted transfer into opens new avenues experimental translational neuroscience lifespan.

Язык: Английский

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Development of a 24-Channel 3T Phased-Array Coil for fMRI in Awake Monkeys - Mitigating Spatiotemporal Artifacts in Ferumoxytol-Weighted Functional Connectivity Estimation

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown

Опубликована: Апрель 20, 2025

Abstract Functional magnetic resonance imaging (fMRI) of awake macaque monkeys holds promise for advancing our understanding primate brain organization, including humans. However, estimating functional connectivity in animals is challenging due to the limited duration sessions and relatively low sensitivity neural activity. To overcome these challenges, we developed a 24-channel 3T receive radiofrequency (RF) coil optimized parallel macaques. This enabled acquisition multiband GRAPPA-accelerated ferumoxytol-weighted resting-state fMRI. The Human Connectome Project-style data processing pipelines were adapted address unique preprocessing demands cerebral blood volume-weighted (CBVw) imaging, motion correction, functional-to-structural image co-registration, training multi-run independent component analysis-based X-noiseifier (ICA-FIX) classifier removal structured artifacts. Our CBVw fMRI approach resulted an elevated contrast-to-noise ratio compared oxygenation level dependent (BOLD) anesthetized artifacts still contributed more variance timeseries than By applying ICA-FIX classifier, achieved highly reproducible parcellated at single-subject level. At group-level, identified dense networks with spatial features homologous those observed RF coil, protocols, analysis are publicly available, providing broader scientific community tools leverage advances further research.

Язык: Английский

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Cortical adaptation of the night monkey to a nocturnal niche environment: a comparative non-invasive T1w/T2w myelin study

Brain Structure and Function, Год журнала: 2022, Номер 228(5), С. 1107 - 1123

Опубликована: Ноя. 18, 2022

Night monkeys (Aotus) are the only genus of within Simian lineage that successfully occupy a nocturnal environmental niche. Their behavior is supported by their sensory organs' distinctive morphological features; however, little known about evolutionary adaptations in regions cerebral cortex. Here, we investigate this question exploring cortical organization night using high-resolution in-vivo brain MRI and comparative cortical-surface T1w/T2w myeloarchitectonic mapping. Our results show monkey cortex has qualitatively similar but quantitatively different pattern myelin compared to diurnal macaque marmoset monkeys. its gradient allowed us parcellate high areas, including middle temporal complex (MT +) auditory cortex, low-myelin area, Brodmann area 7 (BA7) three species, despite species differences convolutions. Relative total those MT + significantly larger than monkeys, whereas BA7 occupies fraction sheet all species. We propose selective expansion areas dedicated visual motion processing may reflect environment.

Язык: Английский

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Naturalistic movies and encoding analysis define areal borders in marmoset third-tier visual cortex

Progress in Neurobiology, Год журнала: 2024, Номер 240, С. 102657 - 102657

Опубликована: Авг. 3, 2024

Accurate definition of the borders cortical visual areas is essential for study neuronal processes leading to perception. However, data used areal boundaries have suffered from issues related resolution, uniform coverage, or suitability objective analysis, ambiguity. Here, we present a novel approach that combines widefield optical imaging, presentation naturalistic movies, and encoding model objectively define in primate extrastriate cortex. We applied this method test conflicting hypotheses about third-tier cortex, where remained controversial. demonstrate pronounced tuning preferences areas, an organizational structure which dorsomedial area (DM) contains representations both upper lower contralateral quadrants, located immediate anterior V2. High-density electrophysiological recordings with Neuropixels probe confirm these findings. Our encoding-model offers powerful, way disambiguate boundaries.

Язык: Английский

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Systematic cortical thickness and curvature patterns in primates

NeuroImage, Год журнала: 2023, Номер 278, С. 120283 - 120283

Опубликована: Июль 27, 2023

Humans are known to have significant and consistent differences in thickness throughout the cortex, with thick outer gyral folds thin inner sulcal folds. Our previous work has suggested a mechanical basis for this pattern, forces generated during cortical folding leading gyri sulci, shown that varies along gyral-sulcal spectrum humans. While other primate species expected exhibit similar patterns of thickness, it is currently unknown how these scale across different sizes, forms, foldedness. Among primates, brains vary enormously from roughly size grape grapefruit, nearly smooth dramatically folded; these, human largest most folded. These variations form make comparative neuroanatomy rich resource investigating common trends transcend between species. In study, we examine 12 order cover wide range sizes investigate scaling their relative surface geometry. The were selected due public availability either reconstructed surfaces and/or population templates. After obtaining or reconstructing 3D publicly available neuroimaging data, used our surface-based computational pipeline (https://github.com/mholla/curveball) analyze respect (total area), geometry (i.e. curvature, shape, depth), foldedness (gyrification). all species, found spectrum, convex shapes thicker than concave saddle between. Furthermore, saw an increasing difference sulci as brain increases. results suggest systematic mechanism relating local Finally, morphometry data future research neuroanatomy.

Язык: Английский

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A method for chronic and semi-chronic microelectrode array implantation in deep brain structures using image guided neuronavigation

Journal of Neuroscience Methods, Год журнала: 2023, Номер 397, С. 109948 - 109948

Опубликована: Авг. 10, 2023

Accurate targeting of brain structures for in-vivo electrophysiological recordings is essential basic as well clinical neuroscience research. Although methodologies precise and recording from the cortical surface are abundant, such protocols scarce deep structures.We have incorporated stable fiducial markers within a custom cranial cap improved image-guided neuronavigation subcortical in macaque monkeys. Anchor bolt chambers allowed minimally invasive entrance into chronic recordings. A 3D-printed microdrive semi-chronic applications.We achieved an average Euclidean error 1.6 mm radial 1.2 over three implantations two animals. Chronic extracellular neuronal activity, with single-neuron activity examples shown one monkey.Traditional stereotactic methods ignore individual anatomical variability. Our approach allows flexible, subject-specific surgical plan errors lower than what reported humans, equal to or animal models using similar methods. Utilizing anchor chamber reduced craniotomy size needed electrode implantation, compared conventional large access which prone infection. Installation in-house, 3D-printed, screw-to-mount mechanical contrast existing requiring fabrication, assembly, installation complex parts.Leveraging commercially available tools our protocol decreases risk infection open craniotomies, improves accuracy models.

Язык: Английский

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Dynamics of motor direction representation in the primate premotor and primary motor cortices during sensorimotor learning

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Сен. 14, 2023

Summary Sensorimotor learning requires reorganization of neuronal activity in the premotor cortex (PM) and primary motor (M1). However, how PM- M1-specific occurs primates remains unclear. We conducted calcium imaging these areas common marmosets while they learned a two-target reaching (pull/push) task. Throughout learning, dorsorostral PM (PMdr) showed peak earlier than dorsocaudal (PMdc) M1. PMdr decreased representation newly introduced (push) movement, whereas PMdc M1 maintained high representation. Many task-related neurons exhibited strong preference to either movement direction. dynamically switched their preferred direction, stably retained Differences direction between adjacent increased during learning. These results suggest that primate sensorimotor dynamic converts cognitive signals stable specific

Язык: Английский

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