Drosophila neuropeptides in regulation of physiology and behavior

Progress in Neurobiology, Год журнала: 2010, Номер 92(1), С. 42 - 104

Опубликована: Май 5, 2010

Язык: Английский

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Genetic Manipulation of Genes and Cells in the Nervous System of the Fruit Fly

Neuron, Год журнала: 2011, Номер 72(2), С. 202 - 230

Опубликована: Окт. 1, 2011

Язык: Английский

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Circadian Organization of Behavior and Physiology in Drosophila

Annual Review of Physiology, Год журнала: 2010, Номер 72(1), С. 605 - 624

Опубликована: Фев. 11, 2010

Circadian clocks organize behavior and physiology to adapt daily environmental cycles. Genetic approaches in the fruit fly, Drosophila melanogaster, have revealed widely conserved molecular gears of these 24-h timers. Yet much less is known about how cell-autonomous confer temporal information modulate cellular functions. Here we discuss our current knowledge circadian clock function Drosophila, providing an overview underpinnings clocks. We then describe neural network important for rhythms locomotor activity, including might influence neuronal function. Finally, address a range behaviors physiological systems regulated by clocks, discussion specific peripheral oscillators key effectors where they been described. These studies reveal remarkable complexity pathways this "simple" model organism.

Язык: Английский

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PDF Cells Are a GABA-Responsive Wake-Promoting Component of the Drosophila Sleep Circuit

Neuron, Год журнала: 2008, Номер 60(4), С. 672 - 682

Опубликована: Ноя. 1, 2008

Язык: Английский

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Circadian Rhythms and Sleep in Drosophila melanogaster

Genetics, Год журнала: 2017, Номер 205(4), С. 1373 - 1397

Опубликована: Март 30, 2017

The advantages of the model organism Drosophila melanogaster, including low genetic redundancy, functional simplicity, and ability to conduct large-scale screens, have been essential for understanding molecular nature circadian (∼24 hr) rhythms, continue be valuable in discovering novel regulators rhythms sleep. In this review, we discuss current these interrelated biological processes wider implications research. Clock genes period timeless were first discovered screens developed 1970s. Feedback on their own transcription forms core clock, accurately timed expression, localization, post-transcriptional modification, function is thought critical maintaining cycle. Regulators, several phosphatases kinases, act different steps feedback loop ensure strong rhythms. Approximately 150 neurons fly brain that contain components clock together translate intracellular cycling into rhythmic behavior. We how groups serve functions allowing clocks entrain environmental cues, driving behavioral outputs at times day, flexible responses conditions. neuropeptide PDF provides an important signal synchronize neurons, although details accomplishes are still being explored. Secreted signals from also influence other tissues. SLEEP is, part, regulated by which ensures appropriate timing sleep, but amount quality sleep determined mechanisms a homeostatic balance between wake. Flies useful identifying large set genes, molecules, neuroanatomic loci regulating amount. Conserved aspects regulation flies mammals include wake-promoting roles catecholamine neurotransmitters involvement hypothalamus-like regions, regions implicated less clear parallels. Sleep subject factors such as food availability, stress, social environment. beginning understand identified molecules interact with each other, environment, regulate researchers can take advantage increasing mechanistic behaviors, learning memory, courtship, aggression, loss impacts behaviors. thus remain tool both discovery deep

Язык: Английский

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Visualizing Neuromodulation In Vivo: TANGO-Mapping of Dopamine Signaling Reveals Appetite Control of Sugar Sensing

Cell, Год журнала: 2012, Номер 148(3), С. 583 - 595

Опубликована: Фев. 1, 2012

Язык: Английский

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Recent advances in neuropeptide signaling in Drosophila, from genes to physiology and behavior

Progress in Neurobiology, Год журнала: 2019, Номер 179, С. 101607 - 101607

Опубликована: Март 22, 2019

Язык: Английский

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Fluorescence/Bioluminescence Resonance Energy Transfer Techniques to Study G-Protein-Coupled Receptor Activation and Signaling

Pharmacological Reviews, Год журнала: 2012, Номер 64(2), С. 299 - 336

Опубликована: Март 8, 2012

Fluorescence and bioluminescence resonance energy transfer (FRET BRET) techniques allow the sensitive monitoring of distances between two labels at nanometer scale. Depending on placement labels, this permits analysis conformational changes within a single protein (for example receptor) or protein-protein interactions example, receptors G-protein subunits). Over past decade, numerous such have been developed to monitor activation signaling G-protein-coupled (GPCRs) in both purified, reconstituted state intact cells. These span entire spectrum from ligand binding down intracellular second messengers. They determination visualization processes with high temporal spatial resolution. With these techniques, it has demonstrated that GPCR signals may show patterning. In particular, evidence provided for compartmentalization GPCRs their cells distinct physiological consequences We review here FRET BRET technologies proteins (G-proteins, effectors) concepts result experiments.

Язык: Английский

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Identification of a dopamine pathway that regulates sleep and arousal in Drosophila

Nature Neuroscience, Год журнала: 2012, Номер 15(11), С. 1516 - 1523

Опубликована: Окт. 14, 2012

Язык: Английский

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Light-arousal and circadian photoreception circuits intersect at the large PDF cells of the Drosophila brain

Proceedings of the National Academy of Sciences, Год журнала: 2008, Номер 105(50), С. 19587 - 19594

Опубликована: Дек. 6, 2008

The neural circuits that regulate sleep and arousal as well their integration with circadian remain unclear, especially in Drosophila. This issue intersects of photoreception, because light is both an signal diurnal animals entraining for the clock. To identify neurons relevant to light-mediated phase-shifting, we developed genetic techniques link behavior single cell-type resolution within Drosophila central brain. We focused on unknown function 10 PDF-containing large ventral lateral (l-LNvs) brain network show here these cells light-dependent arousal. They also are important phase shifting late-night (dawn), indicating photoresponse a property therefore non-cell-autonomous. data further indicate underlying light-induced photoentrainment intersect at l-LNvs then segregate.

Язык: Английский

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