Comprehensive Classification of Retinal Bipolar Neurons by Single-Cell Transcriptomics

Cell, Год журнала: 2016, Номер 166(5), С. 1308 - 1323.e30

Опубликована: Авг. 1, 2016

Язык: Английский

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The Types of Retinal Ganglion Cells: Current Status and Implications for Neuronal Classification

Annual Review of Neuroscience, Год журнала: 2015, Номер 38(1), С. 221 - 246

Опубликована: Апрель 21, 2015

In the retina, photoreceptors pass visual information to interneurons, which process it and retinal ganglion cells (RGCs). Axons of RGCs then travel through optic nerve, telling rest brain all will ever know about world. Research over past several decades has made clear that most are not merely light detectors, but rather feature send a diverse set parallel, highly processed images world on higher centers. Here, we review progress in classification by physiological, morphological, molecular criteria, making particular effort distinguish those cell types definitive from for is partial. We focus mouse, genetic methods advanced. argue there around 30 RGC can now account well half RGCs. also use examine general problem neuronal classification, arguing insights retina guide enterprise other regions.

Язык: Английский

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Intrinsic Control of Axon Regeneration

Neuron, Год журнала: 2016, Номер 90(3), С. 437 - 451

Опубликована: Май 1, 2016

Язык: Английский

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Single-Cell Profiles of Retinal Ganglion Cells Differing in Resilience to Injury Reveal Neuroprotective Genes

Neuron, Год журнала: 2019, Номер 104(6), С. 1039 - 1055.e12

Опубликована: Ноя. 26, 2019

Язык: Английский

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Intrinsic mechanisms of neuronal axon regeneration

Nature reviews. Neuroscience, Год журнала: 2018, Номер 19(6), С. 323 - 337

Опубликована: Апрель 17, 2018

Язык: Английский

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Required growth facilitators propel axon regeneration across complete spinal cord injury

Nature, Год журнала: 2018, Номер 561(7723), С. 396 - 400

Опубликована: Авг. 28, 2018

Язык: Английский

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Single cell transcriptome profiling of retinal ganglion cells identifies cellular subtypes

Nature Communications, Год журнала: 2018, Номер 9(1)

Опубликована: Июль 11, 2018

Retinal ganglion cells (RGCs) convey the major output of information collected from eye to brain. Thirty subtypes RGCs have been identified date. Here, we analyze 6225 (average 5000 genes per cell) right and left eyes by single-cell RNA-seq classify them into 40 using clustering algorithms. We identify additional markers, as well transcription factors predicted cooperate in specifying RGC subtypes. Zic1, a marker eye-enriched subtype, is validated immunostaining situ. Runx1 Fst, markers other subtypes, are purified fluorescent situ hybridization (FISH) immunostaining. show extent gene expression variability needed for subtype segregation, hierarchy diversification cell-type population Finally, present website comparing

Язык: Английский

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Bone Marrow-Derived Mesenchymal Stem Cells-Derived Exosomes Promote Survival of Retinal Ganglion Cells Through miRNA-Dependent Mechanisms

Stem Cells Translational Medicine, Год журнала: 2017, Номер 6(4), С. 1273 - 1285

Опубликована: Янв. 26, 2017

Abstract The loss of retinal ganglion cells (RGC) and their axons is one the leading causes blindness includes traumatic (optic neuropathy) degenerative (glaucoma) eye diseases. Although no clinical therapies are in use, mesenchymal stem (MSC) have demonstrated significant neuroprotective axogenic effects on RGC both aforementioned models. Recent evidence has shown that MSC secrete exosomes, membrane enclosed vesicles (30–100 nm) containing proteins, mRNA miRNA which can be delivered to nearby cells. present study aimed isolate exosomes from bone marrow-derived (BMSC) test them a rat optic nerve crush (ONC) model. Treatment primary cultures with BMSC-exosomes neuritogenic effects. Twenty-one days after ONC weekly intravitreal exosome injections; optical coherence tomography, electroretinography, immunohistochemistry was performed. BMSC-derived promoted statistically survival regeneration while partially preventing axonal dysfunction. Exosomes successfully cargo into inner layers were reliant miRNA, by diminished therapeutic derived BMSC knockdown Argonaute-2, key effector molecule. This supports use as cell-free therapy for ocular disease.

Язык: Английский

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Epitranscriptomic m6A Regulation of Axon Regeneration in the Adult Mammalian Nervous System

Neuron, Год журнала: 2018, Номер 97(2), С. 313 - 325.e6

Опубликована: Янв. 1, 2018

Язык: Английский

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Neural activity promotes long-distance, target-specific regeneration of adult retinal axons

Nature Neuroscience, Год журнала: 2016, Номер 19(8), С. 1073 - 1084

Опубликована: Июль 12, 2016

Язык: Английский

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