Distinct reward processing by subregions of the nucleus accumbens

Cell Reports, Год журнала: 2023, Номер 42(2), С. 112069 - 112069

Опубликована: Фев. 1, 2023

The nucleus accumbens (NAc) plays an important role in motivation and reward processing. Recent studies suggest that different NAc subnuclei differentially contribute to reward-related behaviors. However, how is encoded individual neurons remains unclear. Using vivo single-cell resolution calcium imaging, we find diverse patterns of encoding the medial lateral shell subdivision (NAcMed NAcLat, respectively). Reward consumption increases NAcLat activity but decreases NAcMed activity, albeit with high variability among neurons. heterogeneity could be attributed differences their synaptic inputs transcriptional profiles. Specific optogenetic activation Nts-positive promotes positive reinforcement, while Cartpt-positive induces behavior aversion. Collectively, our study shows organizational subregions provides a framework for future dissection physiological pathological conditions.

Язык: Английский

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Ventral pallidum GABA and glutamate neurons drive approach and avoidance through distinct modulation of VTA cell types

Nature Communications, Год журнала: 2024, Номер 15(1)

Опубликована: Май 18, 2024

Abstract The ventral pallidum (VP) contains GABA and glutamate neurons projecting to tegmental area (VTA) whose stimulation drives approach avoidance, respectively. Yet little is known about the mechanisms by which VP cell types shape VTA activity drive behavior. Here, we found that both were activated during reward or delivery of an aversive stimulus. Stimulation inhibited GABA, but dopamine neurons. Remarkably, stimulation-evoked activation was behavior-contingent such recruitment when evoked subject’s own action. Conversely, as well neurons, despite driving aversion. However, in aversion-associated ventromedial nucleus accumbens (NAc), reduced release reward-associated dorsomedial NAc. These findings show how heterogeneous projections can be engaged avoidance behaviors.

Язык: Английский

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Unlocking opioid neuropeptide dynamics with genetically encoded biosensors

Nature Neuroscience, Год журнала: 2024, Номер 27(9), С. 1844 - 1857

Опубликована: Июль 15, 2024

Abstract Neuropeptides are ubiquitous in the nervous system. Research into neuropeptides has been limited by a lack of experimental tools that allow for precise dissection their complex and diverse dynamics circuit-specific manner. Opioid peptides modulate pain, reward aversion as such have high clinical relevance. To illuminate spatiotemporal endogenous opioid signaling brain, we developed class genetically encoded fluorescence sensors based on kappa, delta mu receptors: κLight, δLight µLight, respectively. We characterized pharmacological profiles these mammalian cells dissociated neurons. used κLight to identify electrical stimulation parameters trigger release scale dynorphin volume transmission brain slices. Using vivo fiber photometry mice, demonstrated utility detecting optogenetically driven observed differential response fearful rewarding conditions.

Язык: Английский

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Sequential transitions of male sexual behaviors driven by dual acetylcholine-dopamine dynamics

Neuron, Год журнала: 2025, Номер unknown

Опубликована: Март 1, 2025

The neural mechanisms underlying the sequential transitions of male sexual behaviors, including mounting, intromission, and ejaculation, remain largely unexplored. Here, we report that acetylcholine (ACh)-dopamine (DA) dynamics in ventral shell nucleus accumbens (vsNAc) regulate these mice. During vsNAc displays a unique pattern dual ACh-DA rhythms, generated by reciprocal regulation between ACh DA signaling via nicotinic receptors (nAChRs) D2 (D2Rs). Knockdown choline acetyltransferase (ChAT) or D2R diminishes occurrence intromission ejaculation. Optogenetic manipulations demonstrated maintains behaviors suppressing D2RvsNAc neurons. Moreover, promotes initiation mounting facilitates intromission-ejaculation transition inducing slowdown rhythm. Together, findings reveal coordinated play critical role orchestrating behaviors.

Язык: Английский

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Reappraising striatal D1- and D2-neurons in reward and aversion

Neuroscience & Biobehavioral Reviews, Год журнала: 2016, Номер 68, С. 370 - 386

Опубликована: Май 25, 2016

Язык: Английский

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