Simultaneous cortex-wide fluorescence Ca2+ imaging and whole-brain fMRI

Nature Methods, Год журнала: 2020, Номер 17(12), С. 1262 - 1271

Опубликована: Ноя. 2, 2020

Язык: Английский

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Resting-state “physiological networks”

NeuroImage, Год журнала: 2020, Номер 213, С. 116707 - 116707

Опубликована: Март 9, 2020

Slow changes in systemic brain physiology can elicit large fluctuations fMRI time series, which manifest as structured spatial patterns of temporal correlations between distant regions. Here, we investigated whether such "physiological networks"—sets segregated regions that exhibit similar responses following slow physiology—resemble associated with large-scale networks typically attributed to remotely synchronized neuronal activity. By analyzing a group subjects from the 3T Human Connectome Project (HCP) database, demonstrate brain-wide and noticeably heterogenous dynamics tightly coupled either respiratory variation or heart rate changes. We show, using synthesized data generated physiological recordings across subjects, these physiologically-coupled alone produce strongly resemble previously reported resting-state networks, suggesting that, some cases, networks" seem mimic networks. Further, show physiologically-relevant connectivity estimates appear dominate overall observations multiple HCP this apparent connectivity" cannot be removed by use single nuisance regressor for entire (such global signal regression) due clear regional heterogeneity responses. Our results challenge previous notions confounds are localized veins globally coherent cortex, therefore emphasizing necessity consider potential contributions fMRI-based functional studies. The rich spatiotemporal carried "physiological" also suggest great clinical biomarkers complementary

Язык: Английский

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Pericyte Control of Blood Flow Across Microvascular Zones in the Central Nervous System

Annual Review of Physiology, Год журнала: 2021, Номер 84(1), С. 331 - 354

Опубликована: Окт. 21, 2021

The vast majority of the brain's vascular length is composed capillaries, where our understanding blood flow control remains incomplete. This review synthesizes current knowledge on across microvascular zones by addressing issues with nomenclature and drawing new developments from in vivo optical imaging single-cell transcriptomics. Recent studies have highlighted important distinctions mural cell morphology, gene expression, contractile dynamics, which can explain observed differences response to vasoactive mediators between arteriole, transitional, capillary zones. Smooth muscle cells arterioles ensheathing pericytes arteriole-capillary transitional zone large-scale, rapid changes flow. In contrast, downstream act slower smaller scales are involved establishing resting tone heterogeneity. Many unresolved remain, including that activate different pericyte types vivo, role pericyte-endothelial communication conducting signals capillaries arterioles, how neurological disease affects these mechanisms.

Язык: Английский

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Receptor-informed network control theory links LSD and psilocybin to a flattening of the brain’s control energy landscape

Nature Communications, Год журнала: 2022, Номер 13(1)

Опубликована: Окт. 3, 2022

Psychedelics including lysergic acid diethylamide (LSD) and psilocybin temporarily alter subjective experience through their neurochemical effects. Serotonin 2a (5-HT2a) receptor agonism by these compounds is associated with more diverse (entropic) brain activity. We postulate that this increase in entropy may arise part from a flattening of the brain's control energy landscape, which can be observed using network theory to quantify required transition between recurrent states. Using states derived existing functional magnetic resonance imaging (fMRI) datasets, we show LSD reduce for state transitions compared placebo. Furthermore, across individuals, reduction correlates frequent increased dynamics. Through analysis incorporates spatial distribution 5-HT2a receptors (obtained publicly available positron emission tomography (PET) data under non-drug conditions), demonstrate an association reduced energy. Our findings provide evidence agonist allow facile temporally More broadly, receptor-informed model impact neuropharmacological manipulation on activity

Язык: Английский

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Multisensory gamma stimulation promotes glymphatic clearance of amyloid

Nature, Год журнала: 2024, Номер 627(8002), С. 149 - 156

Опубликована: Фев. 28, 2024

Abstract The glymphatic movement of fluid through the brain removes metabolic waste 1–4 . Noninvasive 40 Hz stimulation promotes neural activity in multiple regions and attenuates pathology mouse models Alzheimer’s disease 5–8 Here we show that multisensory gamma influx cerebrospinal efflux interstitial cortex 5XFAD model disease. Influx was associated with increased aquaporin-4 polarization along astrocytic endfeet dilated meningeal lymphatic vessels. Inhibiting clearance abolished removal amyloid by stimulation. Using chemogenetic manipulation a genetically encoded sensor for neuropeptide signalling, found vasoactive intestinal peptide interneurons facilitate regulating arterial pulsatility. Our findings establish novel mechanisms recruit system to remove amyloid.

Язык: Английский

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Why is everyone talking about brain state?

Trends in Neurosciences, Год журнала: 2023, Номер 46(7), С. 508 - 524

Опубликована: Май 8, 2023

The rapid and coordinated propagation of neural activity across the brain provides foundation for complex behavior cognition. Technical advances neuroscience subfields have advanced understanding these dynamics, but points convergence are often obscured by semantic differences, creating silos subfield-specific findings. In this review we describe how a parsimonious conceptualization state as fundamental building block whole-brain offers common framework to relate findings scales species. We present examples diverse techniques commonly used study states associated with physiology higher-order cognitive processes, discuss integration them will enable more comprehensive mechanistic characterization dynamics that crucial survival disrupted in disease.

Язык: Английский

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Increased fMRI connectivity upon chemogenetic inhibition of the mouse prefrontal cortex

Nature Communications, Год журнала: 2022, Номер 13(1)

Опубликована: Фев. 25, 2022

Abstract While shaped and constrained by axonal connections, fMRI-based functional connectivity reorganizes in response to varying interareal input or pathological perturbations. However, the causal contribution of regional brain activity whole-brain fMRI network organization remains unclear. Here we combine neural manipulations, resting-state vivo electrophysiology probe how inactivation a cortical node causally affects brain-wide coupling mouse. We find that chronic inhibition medial prefrontal cortex (PFC) via overexpression potassium channel increases between inhibited area its direct thalamo-cortical targets. Acute chemogenetic PFC produces analogous patterns overconnectivity. Using electrophysiology, enhances low frequency (0.1–4 Hz) oscillatory power suppression firing not phase-locked slow rhythms, resulting increased δ band coherence areas exhibit These results provide evidence can counterintuitively increase enhanced, less-localized processes.

Язык: Английский

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Imaging perivascular space structure and function using brain MRI

NeuroImage, Год журнала: 2022, Номер 257, С. 119329 - 119329

Опубликована: Май 21, 2022

In this article, we provide an overview of current neuroimaging methods for studying perivascular spaces (PVS) in humans using brain MRI. recent years, increasing number studies highlighted the role PVS cerebrospinal/interstial fluid circulation and clearance cerebral waste products their association with neurological diseases. Novel strategies techniques have been introduced to improve quantification investigate function morphological features physiological pathological conditions. After a brief introduction on anatomy physiology PVS, examine latest technological developments quantitatively analyze structure We describe applications, advantages, limitations these methods, providing guidance suggestions acquisition protocols analysis that can be applied study vivo. Finally, review human across normative lifespan context disorders.

Язык: Английский

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Blood pressure pulsations modulate central neuronal activity via mechanosensitive ion channels

Science, Год журнала: 2024, Номер 383(6682)

Опубликована: Фев. 1, 2024

The transmission of the heartbeat through cerebral vascular system causes intracranial pressure pulsations. We discovered that arterial pulsations can directly modulate central neuronal activity. In a semi-intact rat brain preparation, elicited correlated local field oscillations in olfactory bulb mitral cell layer. These did not require synaptic but reflected baroreceptive transduction cells. This was mediated by fast excitatory mechanosensitive ion channel and modulated spiking awake animals, entrained activity subset neurons within ~20 milliseconds. Thus, we propose this fast, intrinsic interoceptive mechanism perception-for example, during arousal-within possibly across various other areas.

Язык: Английский

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Intrinsic macroscale oscillatory modes driving long range functional connectivity in female rat brains detected by ultrafast fMRI

Nature Communications, Год журнала: 2023, Номер 14(1)

Опубликована: Фев. 6, 2023

Abstract Spontaneous fluctuations in functional magnetic resonance imaging (fMRI) signals correlate across distant brain areas, shaping functionally relevant intrinsic networks. However, the generative mechanism of fMRI signal correlations, and particular link with locally-detected ultra-slow oscillations, are not fully understood. To investigate this link, we record ultrafast ultrahigh field (9.4 Tesla, temporal resolution = 38 milliseconds) from female rats three anesthesia conditions. Power at frequencies extending up to 0.3 Hz is detected consistently rat brains modulated by level. Principal component analysis reveals a repertoire modes, which transient oscillations organize fixed phase relationships distinct cortical subcortical structures. Oscillatory modes found vary between conditions, resonating faster under medetomidine sedation reducing both number, frequency, duration addition isoflurane. Peaking power within clear anatomical boundaries, these oscillatory point an emergent systemic property. This work provides additional insight into origin organizing principles underpinning spontaneous long-range connectivity.

Язык: Английский

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