110 μm thin endo-microscope for deep-brain in vivo observations of neuronal connectivity, activity and blood flow dynamics

Nature Communications, Год журнала: 2023, Номер 14(1)

Опубликована: Апрель 5, 2023

Abstract Light-based in-vivo brain imaging relies on light transport over large distances of highly scattering tissues. Scattering gradually reduces contrast and resolution, making it difficult to reach structures at greater depths even with the use multiphoton techniques. To deeper, minimally invasive endo-microscopy techniques have been established. These most commonly exploit graded-index rod lenses enable a variety modalities in head-fixed freely moving animals. A recently proposed alternative is holographic control through multimode optical fibres promising much less traumatic application superior performance. We present 110 μm thin laser-scanning endo-microscope based this prospect, enabling volumetric throughout whole depth mouse brain. The instrument equipped multi-wavelength detection three-dimensional random access options, performs lateral resolution below 1 μm. showcase various modes its observations fluorescently labelled neurones, their processes blood vessels. Finally, we demonstrate how monitor calcium signalling neurones measure flow velocity individual vessels high speeds.

Язык: Английский

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Norepinephrine-mediated slow vasomotion drives glymphatic clearance during sleep

Cell, Год журнала: 2025, Номер unknown

Опубликована: Янв. 1, 2025

Язык: Английский

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New perspectives on the glymphatic system and the relationship between glymphatic system and neurodegenerative diseases

Neurobiology of Disease, Год журнала: 2025, Номер 205, С. 106791 - 106791

Опубликована: Янв. 6, 2025

Neurodegenerative diseases (ND) are characterized by the accumulation of aggregated proteins. The glymphatic system, through its rapid exchange mechanisms between cerebrospinal fluid (CSF) and interstitial (ISF), facilitates movement metabolic substances within brain, serving functions akin to those peripheral lymphatic system. This emerging waste clearance mechanism offers a novel perspective on removal pathological in ND. article elucidates recent discoveries regarding system updates relevant concepts model. It discusses potential roles ND, including Alzheimer's disease (AD), Parkinson's (PD), multiple atrophy (MSA), proposes as therapeutic target for these conditions.

Язык: Английский

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Intracranial Electrophysiology Reveals Reproducible Intrinsic Functional Connectivity within Human Brain Networks

Journal of Neuroscience, Год журнала: 2018, Номер 38(17), С. 4230 - 4242

Опубликована: Апрель 6, 2018

Evidence for intrinsic functional connectivity (FC) within the human brain is largely from neuroimaging studies of hemodynamic activity. Data are lacking anatomically precise electrophysiological recordings in most widely studied nodes networks. Here we used a combination fMRI and electrocorticography (ECoG) five neurosurgical patients with electrodes canonical “default” (medial prefrontal posteromedial cortex), “dorsal attention” (frontal eye fields superior parietal lobule), “frontoparietal control” (inferior lobule dorsolateral cortex) In this unique cohort, simultaneous intracranial these networks were matched across different individuals. Within each network individual, found positive, reproducible, spatial correlation FC measures obtained resting-state separately recorded ECoG same brains. This relationship was reliably identified based on slow (<1 Hz) fluctuations high-frequency broadband (70–170 power, both during wakeful rest sleep. A similar organization often recovered when using lower-frequency (1–70 but anatomical specificity consistency greatest range. An interfrequency comparison revealed that high low-frequency ranges temporally diverged one another, suggesting multiple neurophysiological sources may underlie variations FC. Together, our work offers generalizable basis its dynamics individuals, networks, behavioral states. SIGNIFICANCE STATEMENT The study “rest”, fMRI, now major focus cognitive clinical neuroscience. However, little known about neurophysiology their dynamics. We neural activity sleep directly implanted electrodes. patterns showed striking similarities between direct With improved resolution recordings, also best characterized specific frequencies show profiles within-network over time. Our clarifies how spontaneously organize themselves

Язык: Английский

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Bring the Noise: Reconceptualizing Spontaneous Neural Activity

Trends in Cognitive Sciences, Год журнала: 2020, Номер 24(9), С. 734 - 746

Опубликована: Июнь 27, 2020

Язык: Английский

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