A neuronal ensemble encoding adaptive choice during sensory conflict in Drosophila

Nature Communications, Год журнала: 2021, Номер 12(1)

Опубликована: Июль 5, 2021

Abstract Feeding decisions are fundamental to survival, and decision making is often disrupted in disease. Here, we show that neural activity a small population of neurons projecting the fan-shaped body higher-order central brain region Drosophila represents food choice during sensory conflict. We found deprived flies made tradeoffs between appetitive aversive values food. identified an upstream neuropeptidergic dopaminergic network relays internal state other decision-relevant information specific subset neurons. These were strongly inhibited by taste rejected choice, suggesting they encode behavioral choice. Our findings reveal responses choices determined not only quality, but also previous experience (including outcome) hunger state, which integrated before relay downstream motor circuits for implementation.

Язык: Английский

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Diverse states and stimuli tune olfactory receptor expression levels to modulate food-seeking behavior

eLife, Год журнала: 2022, Номер 11

Опубликована: Авг. 31, 2022

Animals must weigh competing needs and states to generate adaptive behavioral responses the environment. Sensorimotor circuits are thus tasked with integrating diverse external internal cues relevant these context-appropriate behaviors. However, mechanisms that underlie this integration largely unknown. Here, we show a wide range of stimuli converge upon single Caenorhabditis elegans olfactory neuron modulate food-seeking behavior. Using an unbiased ribotagging approach, find expression receptor genes in AWA is influenced by array stimuli, including feeding state, physiological stress, recent sensory cues. We identify odorants activate state-dependent receptors altered influences foraging. Further, dissect molecular neural circuit pathways through which information nutritional state integrated AWA. This reveals modular organization state-related signals arising from different cell types body on independently control chemoreceptor expression. The synthesis allows animals sensorimotor reflect animal's overall state. Our findings suggest general model sensory- transcriptional changes at periphery animals' meet their ongoing states.

Язык: Английский

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Fast, efficient, and accurate neuro-imaging denoising via supervised deep learning

Nature Communications, Год журнала: 2022, Номер 13(1)

Опубликована: Сен. 2, 2022

Volumetric functional imaging is widely used for recording neuron activities in vivo, but there exist tradeoffs between the quality of extracted calcium traces, speed, and laser power. While deep-learning methods have recently been applied to denoise images, their applications downstream analyses, such as recovering high-SNR limited. Further, these require temporally-sequential pre-registered data acquired at ultrafast rates. Here, we demonstrate a supervised deep-denoising method circumvent several applications, including whole-brain imaging, large-field-of-view freely moving animals, complex neurite structures C. elegans. Our framework has 30× smaller memory footprint, fast training inference (50-70 ms); it highly accurate generalizable, further, trained with only small, non-temporally-sequential, independently-acquired datasets (∼500 pairs images). We envision that will enable faster long-term experiments necessary study neuronal mechanisms many behaviors.

Язык: Английский

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Multiphoton imaging of neural structure and activity in Drosophila through the intact cuticle

eLife, Год журнала: 2022, Номер 11

Опубликована: Янв. 24, 2022

We developed a multiphoton imaging method to capture neural structure and activity in behaving flies through the intact cuticle. Our measurements showed that fly head cuticle has surprisingly high transmission at wavelengths >900nm, difficulty of through-cuticle is due air sacs and/or fat tissue underneath By compressing or removing sacs, we performed brain anatomical functional results show 2- 3-photon are comparable superficial regions such as mushroom body, but superior deeper central complex beyond. further demonstrated 2-photon odor-evoked calcium responses from body γ-lobes short term long term. The here extends time limits vivo opens new ways brain.

Язык: Английский

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Associative learning drives longitudinally graded presynaptic plasticity of neurotransmitter release along axonal compartments

eLife, Год журнала: 2022, Номер 11

Опубликована: Март 14, 2022

Anatomical and physiological compartmentalization of neurons is a mechanism to increase the computational capacity circuit, major question what role axonal plays. Axonal may enable localized, presynaptic plasticity alter neuronal output in flexible, experience-dependent manner. Here, we show that olfactory learning generates compartmentalized, bidirectional acetylcholine release varies across longitudinal compartments Drosophila mushroom body (MB) axons. The directionality learning-induced depends on valence event (aversive vs. appetitive), linearly proximal distal following appetitive conditioning, correlates with changes downstream (MBONs) modulate behavioral action selection. Potentiation was dependent Ca V 2.1 calcium channel subunit cacophony . In addition, contrast between positive conditioned stimulus other odors required inositol triphosphate receptor, which maintained responsivity upon repeated presentations, preventing adaptation. Downstream from MB, set MBONs receive their input γ3 MB compartment were for normal learning, suggesting they represent key node through reward influences decision-making. These data demonstrate drives valence-correlated, potentiation, depression synaptic neurotransmitter release, rely distinct mechanisms are distributed circuit.

Язык: Английский

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