Rapid and reversible optogenetic silencing of synaptic transmission by clustering of synaptic vesicles DOI Creative Commons

Dennis Vettkötter,

Martin Schneider, Brady D. Goulden

и другие.

Nature Communications, Год журнала: 2022, Номер 13(1)

Опубликована: Дек. 19, 2022

Abstract Acutely silencing specific neurons informs about their functional roles in circuits and behavior. Existing optogenetic silencers include ion pumps, channels, metabotropic receptors, tools that damage the neurotransmitter release machinery. While former hyperpolarize cell, alter ionic gradients or cellular biochemistry, latter allow only slow recovery, requiring de novo synthesis. Thus, combining fast activation reversibility are needed. Here, we use light-evoked homo-oligomerization of cryptochrome CRY2 to silence synaptic transmission, by clustering vesicles (SVs). We benchmark this tool, optoSynC, Caenorhabditis elegans , zebrafish, murine hippocampal neurons. optoSynC clusters SVs, observable electron microscopy. Locomotion occurs with tau on ~7.2 s recovers off ~6.5 min after light-off. can inhibit exocytosis for several hours, at very low light intensities, does not affect currents, biochemistry proteins, may further manipulating different SV pools transfer SVs between them.

Язык: Английский

Optical control of Class A G protein-coupled receptors with photoswitchable ligands DOI Creative Commons
Maikel Wijtmans, I Josimović, Henry F. Vischer

и другие.

Current Opinion in Pharmacology, Год журнала: 2022, Номер 63, С. 102192 - 102192

Опубликована: Март 4, 2022

The field of photopharmacology Class A GPCR ligands has recently attracted attention. In this review we analyze 31 papers on currently available photoswitchable for GPCRs. Using the six most recurring terms all combined paper abstracts, one can extract overarching goal area research: "Photoswitchable control receptor activity with light" (represented in TOC graphic). We design, photochemistry and pharmacology ligands. Trends, challenges limitations will be discussed. number efficient that allow optical modulation function various vitro assays are presented. Moreover, vivo is within reach first reports to end highlighted.

Язык: Английский

Процитировано

28

Rhodopsins: An Excitingly Versatile Protein Species for Research, Development and Creative Engineering DOI Creative Commons

Willem J. de Grip,

Srividya Ganapathy

Frontiers in Chemistry, Год журнала: 2022, Номер 10

Опубликована: Июнь 22, 2022

The first member and eponym of the rhodopsin family was identified in 1930s as visual pigment rod photoreceptor cell animal retina. It found to be a membrane protein, owing its photosensitivity presence covalently bound chromophoric group. This group, derived from vitamin A, appropriately dubbed retinal. In 1970s microbial counterpart this species discovered an archaeon, being protein also harbouring retinal chromophore, named bacteriorhodopsin. Since their discovery photogenic panorama unfolded, where up date new members subspecies with variety light-driven functionality have been added family. branch, meanwhile categorized type-2 rhodopsins, turned out form large subclass superfamily G protein-coupled receptors are essential multiple elements light-dependent sensory physiology. type-1 largely function ion pumps or channels, but contain sensory-active enzyme-sustaining subspecies. review we will follow development exciting representative number highlights present prospect extraordinary future potential.

Язык: Английский

Процитировано

28

A self-inactivating invertebrate opsin optically drives biased signaling toward Gβγ-dependent ion channel modulation DOI Creative Commons
Hisao Tsukamoto, Yoshihiro Kubo

Proceedings of the National Academy of Sciences, Год журнала: 2023, Номер 120(21)

Опубликована: Май 15, 2023

Animal opsins, light-sensitive G protein-coupled receptors, have been used for optogenetic tools to control protein-dependent signaling pathways. Upon protein activation, the Gα and Gβγ subunits drive different intracellular pathways, leading complex cellular responses. For some purposes, Gα- Gβγ-dependent needs be separately modulated, but these responses are simultaneously evoked due 1:1 stoichiometry of Nevertheless, we show temporal activation using a self-inactivating invertebrate opsin, Platynereis c-opsin1, drives biased GIRK channel in light-dependent manner by utilizing kinetic difference between Gα-dependent The opsin-induced transient Gi/o preferentially causes kinetically fast channels rather than slower Gi/oα-dependent adenylyl cyclase inhibition. Although similar Gβγ-biased properties were observed vertebrate visual pigment, c-opsin1 requires fewer retinal molecules evoke Furthermore, enhanced genetically fusing with RGS8 protein, which accelerates inactivation. opsin its RGS8-fusion can function as optical ion modulation.

Язык: Английский

Процитировано

16

A carotid body-brainstem neural circuit mediates sighing in hypoxia DOI
Yilong Yao, Jingwen Chen, Xingyu Li

и другие.

Current Biology, Год журнала: 2023, Номер 33(5), С. 827 - 837.e4

Опубликована: Фев. 6, 2023

Язык: Английский

Процитировано

14

Rapid and reversible optogenetic silencing of synaptic transmission by clustering of synaptic vesicles DOI Creative Commons

Dennis Vettkötter,

Martin Schneider, Brady D. Goulden

и другие.

Nature Communications, Год журнала: 2022, Номер 13(1)

Опубликована: Дек. 19, 2022

Abstract Acutely silencing specific neurons informs about their functional roles in circuits and behavior. Existing optogenetic silencers include ion pumps, channels, metabotropic receptors, tools that damage the neurotransmitter release machinery. While former hyperpolarize cell, alter ionic gradients or cellular biochemistry, latter allow only slow recovery, requiring de novo synthesis. Thus, combining fast activation reversibility are needed. Here, we use light-evoked homo-oligomerization of cryptochrome CRY2 to silence synaptic transmission, by clustering vesicles (SVs). We benchmark this tool, optoSynC, Caenorhabditis elegans , zebrafish, murine hippocampal neurons. optoSynC clusters SVs, observable electron microscopy. Locomotion occurs with tau on ~7.2 s recovers off ~6.5 min after light-off. can inhibit exocytosis for several hours, at very low light intensities, does not affect currents, biochemistry proteins, may further manipulating different SV pools transfer SVs between them.

Язык: Английский

Процитировано

21