Distinct subpopulations of D1 medium spiny neurons exhibit unique transcriptional responsiveness to cocaine

Molecular and Cellular Neuroscience, Год журнала: 2023, Номер 125, С. 103849 - 103849

Опубликована: Март 24, 2023

Drugs of abuse increase extracellular concentrations dopamine in the nucleus accumbens (NAc), resulting transcriptional alterations that drive long-lasting cellular and behavioral adaptations. While decades research have focused on mechanisms by which drugs influence neuronal physiology function, few studies comprehensively defined NAc cell type heterogeneity responses to abuse. Here, we used single RNA-seq (snRNA-seq) characterize transcriptome over 39,000 cells from male female adult Sprague-Dawley rats following acute or repeated cocaine experience. This dataset identified 16 transcriptionally distinct populations, including two populations medium spiny neurons (MSNs) express Drd1 receptor (D1-MSNs). Critically, while both expressed classic marker genes D1-MSNs, only one population exhibited a robust response cocaine. Validation population-selective transcripts using RNA situ hybridization revealed spatial compartmentalization these D1-MSN within NAc. Finally, analysis published snRNA-seq datasets non-human primates humans demonstrated conservation MSN subtypes across rat higher order mammals, further highlighted type-specific differences broader striatum. These results highlight utility provides useful resource for cross-species comparisons composition.

Язык: Английский

---

Molecular and cellular evolution of the amygdala across species analyzed by single-nucleus transcriptome profiling

Cell Discovery, Год журнала: 2023, Номер 9(1)

Опубликована: Фев. 14, 2023

The amygdala, or an amygdala-like structure, is found in the brains of all vertebrates and plays a critical role survival reproduction. However, cellular architecture amygdala how it has evolved remain elusive. Here, we generated single-nucleus RNA-sequencing data for more than 200,000 cells humans, macaques, mice, chickens. Abundant neuronal cell types from different subnuclei were identified datasets. Cross-species analysis revealed that inhibitory neurons neuron-enriched well-conserved composition marker gene expression, whereas excitatory relatively divergent. Furthermore, LAMP5+ interneurons much abundant primates, while DRD2+ LAMP5+SATB2+ dominant human central amygdalar nucleus (CEA) basolateral complex (BLA), respectively. We also CEA-like their species-specific distribution patterns This study highlights extreme cell-type diversity reveals conservation divergence expression across species may contribute to adaptations.

Язык: Английский

---

Multimodal deep learning approaches for single-cell multi-omics data integration

Briefings in Bioinformatics, Год журнала: 2023, Номер 24(5)

Опубликована: Авг. 14, 2023

Integrating single-cell multi-omics data is a challenging task that has led to new insights into complex cellular systems. Various computational methods have been proposed effectively integrate these rapidly accumulating datasets, including deep learning. However, despite the proven success of learning in integrating and its better performance over classical methods, there no systematic study application integration. To fill this gap, we conducted literature review explore use multimodal techniques integration, taking account recent studies from multiple perspectives. Specifically, first summarized different modalities found data. We then reviewed current for processing categorized learning-based integration according modality, architecture, fusion strategy, key tasks downstream analysis. Finally, provided using models understand biological mechanisms.

Язык: Английский

---

Harmonized cross-species cell atlases of trigeminal and dorsal root ganglia

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Июль 5, 2023

Abstract Peripheral sensory neurons in the dorsal root ganglion (DRG) and trigeminal (TG) are specialized to detect transduce diverse environmental stimuli including touch, temperature, pain central nervous system. Recent advances single-cell RNA-sequencing (scRNA-seq) have provided new insights into diversity of ganglia cell types rodents, non-human primates, humans, but it remains difficult compare transcriptomically defined across studies species. Here, we built cross-species harmonized atlases DRG TG that describe 18 neuronal 11 non-neuronal 6 species 19 studies. We then demonstrate utility this reference atlas by using annotate newly profiled nuclei/cells from both human highly regenerative axolotl. observe transcriptomic profiles neuron subtypes broadly similar vertebrates, expression functionally important neuropeptides channels can vary notably. The resources data presented here guide future comparative transcriptomics, simplify type nomenclature differences studies, help prioritize targets for therapy development.

Язык: Английский

---

Hesperidin from citrus peel waste: extraction and its health implications

Quality Assurance and Safety of Crops & Foods, Год журнала: 2023, Номер 15(2), С. 71 - 99

Опубликована: Апрель 1, 2023

Hesperidin is abundantly present as a flavanone glycoside in citrus fruits. The peels, seeds, pulp, and cell membrane residues contain high amount of hesperidin. It has received scientific momentum lately it offers several health benefits upon consumption, possesses antioxidant, anti-hypocholesteric, antitumor, anticancer, antimicrobial, antibacterial, anti-inflammatory, anti-diabetic properties, so on. used the treatment various diseases disorders such type-II diabetes, cancer, cardiovascular diseases, neurological psychiatric disorders. Various extraction methods solvent extraction, cold microwave-assisted (MAE), ultrasound-assisted (UAE), supercritical fluid have been employed globally to obtain maximum yield hesperidin, which also environment-friendly cost-effective. food industries produce huge residue after preparation juices, jellies, jams, etc. These byproduct wastes are extract This review aims at highlighting updated information about nutritional, phytochemical, biological activities hesperidin; classical modern methods; their impact on hesperidin implications

Язык: Английский

---