Journal of Intensive Medicine,
Год журнала:
2023,
Номер
4(1), С. 73 - 80
Опубликована: Авг. 30, 2023
Intensive
care
unit-acquired
weakness
(ICU-AW)
is
a
common
complication
in
critically
ill
patients
and
associated
with
variety
of
adverse
outcomes.
These
include
the
need
for
prolonged
mechanical
ventilation
ICU
stay;
higher
ICU,
in-hospital,
1-year
mortality;
increased
in-hospital
costs.
ICU-AW
multiple
risk
factors
including
age,
underlying
disease,
severity
illness,
organ
failure,
sepsis,
immobilization,
receipt
ventilation,
other
related
to
critical
care.
The
pathological
mechanism
remains
unclear
may
be
considerably
varied.
This
review
aimed
evaluate
recent
insights
into
from
several
aspects
factors,
pathophysiology,
diagnosis,
treatment
strategies;
this
provides
new
perspectives
future
research.
Monitoring
autophagic
flux
is
necessary
for
most
autophagy
studies.
The
assays
currently
available
mammalian
cells
are
generally
complicated
and
do
not
yield
highly
quantitative
results.
Yeast
routinely
monitored
with
the
green
fluorescence
protein
(GFP)-based
processing
assay,
whereby
amount
of
GFP
proteolytically
released
from
GFP-containing
reporters
(e.g.
GFP-Atg8),
detected
by
immunoblotting,
reflects
flux.
However,
this
simple
effective
assay
typically
inapplicable
to
because
efficiently
degraded
in
lysosomes
while
more
resistant
red
fluorescent
(RFP)
accumulates
under
basal
conditions.
Here,
we
report
a
HaloTag
(Halo)-based
reporter
monitor
We
found
that
Halo
sensitive
lysosomal
proteolysis
but
becomes
upon
ligand
binding.
When
delivered
into
autophagy,
pulse-labeled
Halo-based
Halo-LC3
Halo-GFP)
processed
generate
when
autophagy.
Hence,
free
immunoblotting
or
in-gel
imaging
demonstrate
applications
monitoring
pathways,
macroautophagy,
selective
even
bulk
nonselective
With
lysosome-mediated
degradation
can
be
easily
precisely.
Neuron,
Год журнала:
2024,
Номер
112(8), С. 1208 - 1221
Опубликована: Фев. 22, 2024
Alzheimer's
disease
(AD)
and
the
mechanisms
underlying
its
etiology
progression
are
complex
multifactorial.
The
higher
AD
risk
in
women
may
serve
as
a
clue
to
better
understand
these
complicated
processes.
In
this
review,
we
examine
aspects
of
that
demonstrate
sex-dependent
effects
delve
into
potential
biological
responsible,
compiling
findings
from
advanced
technologies
such
single-cell
RNA
sequencing,
metabolomics,
multi-omics
analyses.
We
review
evidence
sex
hormones
chromosomes
interact
with
various
during
aging,
encompassing
inflammation,
metabolism,
autophagy,
leading
unique
characteristics
between
men
women.
Current Biology,
Год журнала:
2022,
Номер
32(24), С. R1357 - R1371
Опубликована: Дек. 1, 2022
Cellular
homeostasis
requires
the
swift
and
specific
removal
of
damaged
material.
Selective
autophagy
represents
a
major
pathway
for
degradation
such
cargo
This
is
achieved
by
sequestration
within
double-membrane
vesicles
termed
autophagosomes,
which
form
de
novo
around
subsequently
deliver
their
content
to
lysosomes
degradation.
The
importance
selective
exemplified
various
neurodegenerative
diseases
associated
with
defects
in
this
pathway,
including
Parkinson's
disease,
amyotrophic
lateral
sclerosis,
frontotemporal
dementia.
It
has
become
evident
that
receptors
are
acting
as
Swiss
army
knives
recognizing
cargo,
orchestrating
recruitment
machinery
autophagosome
biogenesis,
closely
aligning
membrane
cargo.
Furthermore,
sequester
ubiquitinated
proteins
into
larger
condensates
upstream
induction.
Here,
we
review
recent
insights
mechanisms
action
focusing
on
roles
sequestosome-like
misfolded,
mitochondria.
We
also
highlight
at
steps
function
result
accumulation
harmful
material
how
knowledge
may
guide
design
future
therapies.
Journal of Integrative Neuroscience,
Год журнала:
2023,
Номер
22(1)
Опубликована: Янв. 16, 2023
The
SH-SY5Y
cell
line
is
a
simple
and
inexpensive
in
vitro
experimental
model
for
studying
Parkinson
disease
(PD).
This
useful
tool
elucidating
pathophysiological
mechanisms
of
PD
the
development
new
pharmacological
therapies.
In
this
review,
we
aim
to
summarize
current
protocols
culturing
differentiation
designs
derived
from
line.
most
efficient
protocol
into
dopaminergic
neurons
seems
be
addition
retinoic
acid
growth
medium,
followed
by
12-O-tetradecanoylphorbol-13-acetate
(TPA)
low
concentration
fetal
bovine
serum.
pathological
changes,
such
as
neuronal
apoptosis
intraneuronal
alpha-synuclein
aggregation,
can
reproduced
either
use
neurotoxic
agents
[such
rotenone,
1-methyl-4-phenylpyridinium
(MPP+),
6-hydroxydopamine]
or
genetic
modification
(transfection
wild-type
mutant
gene,
manipulation
other
genes
involved
PD).
addition,
compounds
with
potential
neuroprotective
role
may
tested
on
neurotoxicity-induced
models.
also
used
testing
prion-like
transmission
microbiota
influence
PD.
conclusion,
represents
basic
but
consistent
first
step
experiments
related
PD,
which
must
confirmation
results
through
more
complex
vivo
Neuron,
Год журнала:
2023,
Номер
111(13), С. 2021 - 2037.e12
Опубликована: Апрель 26, 2023
In
neurodegenerative
diseases,
microglia
switch
to
an
activated
state,
which
results
in
excessive
secretion
of
pro-inflammatory
factors.
Our
work
aims
investigate
how
this
paracrine
signaling
affects
neuronal
function.
Here,
we
show
that
mediate
non-cell-autonomous
inhibition
autophagy,
a
degradative
pathway
critical
for
the
removal
toxic,
aggregate-prone
proteins
accumulating
diseases.
We
found
microglial-derived
CCL-3/-4/-5
bind
and
activate
CCR5,
turn
promotes
mTORC1
activation
disrupts
autophagy
protein
clearance.
CCR5
its
cognate
chemokines
are
upregulated
brains
pre-manifesting
mouse
models
Huntington's
disease
(HD)
tauopathy,
suggesting
pathological
role
microglia-neuronal
axis
early
phases
these
upregulation
is
self-sustaining,
as
CCL5-CCR5
impairs
degradation
itself.
Finally,
pharmacological
or
genetic
rescues
hyperactivation
dysfunction,
ameliorates
HD
tau
pathologies
models.
