Cerebellar circuits for disinhibition and synchronous inhibition DOI Creative Commons

Elizabeth P. Lackey,

Luis Moreira,

Aliya Norton

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Сен. 15, 2023

Abstract The cerebellar cortex contributes to diverse behaviors by transforming mossy fiber inputs into predictions in the form of Purkinje cell (PC) outputs, and then refining those 1 . Molecular layer interneurons (MLIs) account for approximately 80% inhibitory 2 , are vital processing 1,3 MLIs thought primarily inhibit PCs suppress plasticity excitatory synapses onto PCs. also inhibit, electrically coupled to, other 4–7 but functional significance these connections is not known Behavioral studies suggest that cerebellar-dependent learning gated disinhibition PCs, source such has been identified 8 Here we find two recently recognized MLI subtypes MLI1 MLI2, have highly specialized connectivity allows them serve very different roles. MLI1s each other, fire synchronously with on millisecond time scale vivo pause PC firing. MLI2s coupled, they disinhibit well suited gating These findings require a major reevaluation within cerebellum which disinhibition, powerful circuit motif present cerebral elsewhere 9–17 greatly increases computational power flexibility cerebellum. They synchronous firing electrically-coupled helps regulate output pausing firing, shown evoke precisely-timed targets 18

Язык: Английский

Pulsed inhibition of corticospinal excitability by the thalamocortical sleep spindle DOI Creative Commons
Umair Hassan,

Prince Okyere,

Milad Amini Masouleh

и другие.

Brain stimulation, Год журнала: 2025, Номер unknown

Опубликована: Фев. 1, 2025

Thalamocortical sleep spindles, i.e., oscillatory bursts at ∼12-15 Hz of waxing and waning amplitude, are a hallmark feature non-rapid eye movement (NREM) believed to play key role in memory reactivation consolidation. Generated the thalamus projecting neocortex hippocampus, they phasically modulated by neocortical slow oscillations (<1 Hz) turn modulate hippocampal sharp-wave ripples (>80 Hz). This hierarchical cross-frequency nesting, where slower group faster ones into certain excitability phases, may enable phase-dependent plasticity neocortex, spindles have thus been considered windows sleeping brain. However, assumed phasic modulation had not yet demonstrated for spindles. Utilizing recently developed real-time spindle detection algorithm, we applied phase-triggered transcranial magnetic stimulation (TMS) primary motor cortex (M1) hand area characterize corticospinal profile via evoked potentials (MEP). MEPs showed net suppression during driven "pulse inhibition" its falling flank with no inhibition or facilitation peak, rising flank, trough. unidirectional ("asymmetric") occurred on top general sleep-related spindle-free NREM did extend refractory post-spindle periods. We conclude that exert "asymmetric pulsed excitability. These findings targeting methods future studies investigate causal synaptic systems consolidation repetitively relevant phases.

Язык: Английский

Процитировано

0
Time‐Dependent Regulation of Sleep‐Wakefulness and Electroencephalographic Characteristics by Spontaneous Running in Male Mice DOI Open Access
Weiwei Zhou,

Jialin Zhou,

Quan Lu

и другие.

Journal of Sleep Research, Год журнала: 2025, Номер unknown

Опубликована: Март 18, 2025

ABSTRACT The relationship between physical activity and sleep quality is a critical area of investigation, given the importance both behaviours for health disease. Despite common use running wheels to assess circadian rhythms exercise, their impact on has not been thoroughly explored. Here, we present detailed analysis how voluntary affected sleep/wakefulness duration, architecture electrophysiological characteristics in mice. Sequential electroencephalogram (EEG) assessments revealed that elicits progressive alteration sleep/wake configurations, including reduction overall daily time an enhancement consolidation. These modifications exhibited temporal association with intensity activities. observed changes duration partially persist even after discontinuation running. Spontaneous also gradually changed amplitude and/or frequency EEG theta power only during phase but rapid eye movement (REMS). In vivo endoscopic calcium imaging freely behaving mice REMS were accompanied by activation largely shared yet distinctive neuronal cohorts within hippocampal CA1 region, concomitant oscillations behaviours. findings highlight dynamic nature regulation response exercise suggest played pivotal role modulating need balance wakefulness.

Язык: Английский

Процитировано

0
Preoperative Sleep Deprivation Exacerbates Anesthesia/Surgery-induced Abnormal GABAergic Neurotransmission and Neuronal Damage in the Hippocampus in Aged Mice DOI
Yun Li,

Siwen Long,

Jiafeng Yu

и другие.

Molecular Neurobiology, Год журнала: 2025, Номер unknown

Опубликована: Март 19, 2025

Язык: Английский

Процитировано

0
Synchronous Ensembles of Hippocampal CA1 Pyramidal Neurons Associated with Theta but not Ripple Oscillations During Novel Exploration DOI Open Access

En-Li Chen,

Tsai‐Wen Chen, Eric R. Schreiter

и другие.

Опубликована: Апрель 1, 2025

Synchronous neuronal ensembles play a pivotal role in the consolidation of long-term memory hippocampus. However, their organization during acquisition spatial remains less clear. In this study, we used population voltage imaging to investigate synchronization patterns CA1 pyramidal exploration new environment, critical phase for acquisition. We found synchronous comprising approximately 40% neurons, firing simultaneously brief windows (∼25ms) immobility and locomotion novel exploration. Notably, these were not associated with contralateral ripple oscillations but instead phase-locked theta waves recorded region. Moreover, subthreshold membrane potentials neurons exhibited coherent intracellular depolarizing peak at moment synchrony. Among newly formed place cells, pairs more robust displayed distinct place-specific activities. These findings underscore coordinating cells different fields.

Язык: Английский

Процитировано

0
Transcriptomic and spatial GABAergic neuron subtypes in zona incerta mediate distinct innate behaviors DOI Creative Commons

Mengyue Zhu,

Jieqiao Peng,

Mi Wang

и другие.

Nature Communications, Год журнала: 2025, Номер 16(1)

Опубликована: Апрель 1, 2025

Язык: Английский

Процитировано

0
Integrating endocannabinoid signaling, CCK interneurons, and hippocampal circuit dynamics in behaving animals DOI Creative Commons
Shreya Malhotra, Florian Donneger, Jordan S. Farrell

и другие.

Neuron, Год журнала: 2025, Номер unknown

Опубликована: Апрель 1, 2025

Язык: Английский

Процитировано

0
Sleep sharp wave ripple and its functions in memory and synaptic plasticity DOI Creative Commons
Zhiwen Zhou, Hiroaki Norimoto

Neuroscience Research, Год журнала: 2023, Номер 189, С. 20 - 28

Опубликована: Апрель 1, 2023

Язык: Английский

Процитировано

8
Disorganized Inhibitory Dynamics and Functional Connectivity in Hippocampal area CA1 of 22q11.2 Deletion Mutant Mice DOI Creative Commons
Stephanie Herrlinger, Bovey Rao,

Margaret E. Conde Paredes

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Апрель 30, 2024

Individuals with the 22q11.2 deletion syndrome, one of strongest genetic risk factors for schizophrenia, demonstrate cognitive impairments including episodic memory dysfunction. Place cell activity excitatory pyramidal neurons in hippocampus supporting are impaired a mouse model ( Df(16)A +/- ). While dynamics under tight inhibitory control by multiple subtypes GABAergic interneurons, previous studies have predominantly focused on single subtype PV-expressing interneurons; there not yet been describing functional relationships between molecularly identified types mice. Here, we examined interneuron subtype-specific dorsal hippocampal area CA1 mice during random foraging and spatial reward learning tasks. Capitalizing 3D acousto-optical deflector two-photon microscopy post hoc immunohistochemical identification, found that exhibit aberrant responses to locations delayed enrichment extinction. interneurons also carry markedly reduced information subtype-dependent manner. We observed task-dependent changes correlation structure coactivity among subtypes, suggesting broadly disorganized microcircuit functionality mutant Overall, identify widespread heterogeneous alterations learning, reflecting flexibility organization microcircuits. Our study provides critical insights into how schizophrenia-risk mutations affect local-circuit interactions diverse navigation.

Язык: Английский

Процитировано

2
Entorhinal cortex glutamatergic and GABAergic projections bidirectionally control discrimination and generalization of hippocampal representations DOI Open Access
Vincent Robert,

Keelin O’Neil,

Shannon K. Rashid

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Ноя. 11, 2023

Discrimination and generalization are crucial brain-wide functions for memory object recognition that utilize pattern separation completion computations. Circuit mechanisms supporting these operations remain enigmatic. We show lateral entorhinal cortex glutamatergic (LEC GLU ) GABAergic GABA projections essential memory. Silencing LEC during in vivo two-photon imaging increased the population of active CA3 pyramidal cells but decreased activity rates, suggesting a sparse coding function through local inhibition. also place cell remapping between different environments validating this circuit drives context discrimination. Optogenetic mapping confirmed dominant feedforward inhibition to prevent somatic dendritic spikes. However, conjunctively suppresses disinhibit neuron soma selectively boost integrative output recurrent network. thus promotes generalization. Indeed, without disinhibitory input, maps similarity contexts. Our findings provide whereby long-range cortico-hippocampal inputs bidirectionally modulate completion, providing neuronal representations with dynamic range discrimination

Язык: Английский

Процитировано

4
Aberrant hippocampal Ca2+ micro-waves following synapsin-dependent adeno-associated viral expression of Ca2+ indicators DOI Open Access

Nicola Masala,

Manuel Mittag, Eleonora Ambrad Giovannetti

и другие.

Опубликована: Апрель 3, 2024

Genetically encoded calcium indicators (GECIs) such as GCaMP are invaluable tools in neuroscience to monitor neuronal activity using optical imaging. The viral transduction of GECIs is commonly used target expression specific brain regions, can be conveniently with any mouse strain interest without the need for prior crossing a GECI line and avoids potential hazards due chronic during development. A key requirement monitoring an indicator that itself minimally affects activity. Here, common adeno-associated (AAV) procedures, we describe spatially confined aberrant Ca 2+ micro-waves slowly travelling through hippocampus following GCaMP6, GCaMP7 or R-CaMP1.07 driven by synapsin promoter AAV-dependent gene transfer, titre-dependent fashion. developed hippocampal CA1 CA3, but not dentate gyrus (DG) nor neocortex, were typically first observed at 4 weeks after transduction, persisted up least 8 weeks. phenomenon was robust, across laboratories various experimenters setups. Our results indicate depend on titre GECI, density well targeted region. We alternative method which this artifact. show -indicator AAV procedures produce artefactual responses. aim raise awareness field these transduction-induced provide solution.Impact statement: Common induce waves hippocampus.

Язык: Английский

Процитировано

1