Somatosensory-Evoked Early Sharp Waves in the Neonatal Rat Hippocampus

International Journal of Molecular Sciences, Год журнала: 2023, Номер 24(10), С. 8721 - 8721

Опубликована: Май 13, 2023

The developing entorhinal–hippocampal system is embedded within a large-scale bottom-up network, where spontaneous myoclonic movements, presumably via somatosensory feedback, trigger hippocampal early sharp waves (eSPWs). hypothesis, that feedback links movements with eSPWs, implies direct stimulation should also be capable of evoking eSPWs. In this study, we examined responses to electrical the periphery in urethane-anesthetized, immobilized neonatal rat pups using silicone probe recordings. We found ~33% trials evoked local field potential (LFP) and multiple unit activity (MUA) identical somatosensory-evoked eSPWs were delayed from stimulus, on average, by 188 ms. Both (i) had similar amplitude ~0.5 mV half-duration ~40 ms, (ii) current-source density (CSD) profiles, current sinks CA1 strata radiatum, lacunosum-moleculare DG molecular layer (iii) associated MUA increase DG. Our results indicate can triggered stimulations support hypothesis sensory involved association rats.

Язык: Английский

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Cortical and thalamic inputs drive distinct hippocampal microcircuits to modulate synchronized activity during development

Neuron, Год журнала: 2023, Номер 111(6), С. 761 - 763

Опубликована: Март 1, 2023

Язык: Английский

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Chemogenetic silencing reveals presynaptic Gi/o protein-mediated inhibition of synchronized activity in the developing hippocampus in vivo

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Фев. 2, 2024

The development of neuronal circuits is an activity-dependent process. Research aimed at deciphering the learning rules governing these developmental refinements increasingly utilizing a class chemogenetic tools that employ Gi/o protein signaling (Gi-DREADDs) for silencing. However, their mechanisms action and inhibitory efficacy in immature neurons with incompletely developed are poorly understood. Here, we analyze impact on cellular network excitability neonatal hippocampus by expressing Gi-DREADD hM4Di telencephalic glutamatergic mice both sexes. Using acousto-optic two-photon Ca2+ imaging, report activation leads to complete arrest spontaneous synchronized activity CA1 vitro. Electrophysiological analyses demonstrate hM4Di-mediated silencing not accounted changes intrinsic pyramidal cells (PCs). Instead, robustly restrains synaptic glutamate release first postnatal week, effectively reducing recurrent excitation CA1. In vivo, inhibition through potently suppresses early sharp waves (eSPWs) discontinuous oscillatory head-fixed before eye opening. summary, dampens neurotransmission presynaptic mechanism sufficient terminate Our findings have implications designing interpreting experiments Gi-DREADDs further point potential role Gi/o-dependent endogenous neuromodulators hippocampal development.

Язык: Английский

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Chemogenetic silencing reveals presynaptic Gi/o protein-mediated inhibition of developing hippocampal synchrony in vivo

iScience, Год журнала: 2024, Номер 27(10), С. 110997 - 110997

Опубликована: Сен. 20, 2024

Язык: Английский

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