Distinct neurochemical influences on fMRI response polarity in the striatum

Nature Communications, Год журнала: 2024, Номер 15(1)

Опубликована: Март 1, 2024

Abstract The striatum, known as the input nucleus of basal ganglia, is extensively studied for its diverse behavioral roles. However, relationship between neuronal and vascular activity, vital interpreting functional magnetic resonance imaging (fMRI) signals, has not received comprehensive examination within striatum. Here, we demonstrate that optogenetic stimulation dorsal striatal neurons or their afferents from various cortical subcortical regions induces negative fMRI responses in rats, manifesting vasoconstriction. These occur even with heightened confirmed by electrophysiology fiber-photometry. In parallel, midbrain dopaminergic neuron modulation, coupled electrochemical measurements, establishes a link vasodilation dopamine release. Intriguingly, vivo intra-striatal pharmacological manipulations during highlight critical role opioidergic signaling generating This observation substantiated detecting vasoconstriction brain slices after synthetic opioid application. humans, aimed at increasing activity likewise elicit responses. Our results emphasize necessity considering vasoactive neurotransmission alongside when signal.

Язык: Английский

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No replication of direct neuronal activity–related (DIANA) fMRI in anesthetized mice

Science Advances, Год журнала: 2024, Номер 10(13)

Опубликована: Март 27, 2024

Direct imaging of neuronal activity (DIANA) by functional magnetic resonance (fMRI) could be a revolutionary approach for advancing systems neuroscience research. To independently replicate this observation, we performed fMRI experiments in anesthetized mice. The blood oxygenation level–dependent (BOLD) response to whisker stimulation was reliably detected the primary barrel cortex before and after DIANA experiments; however, no DIANA–like peak observed individual animals’ data with 50 300 trials. Extensively averaged involving 1050 trials six mice showed flat baseline detectable activity–like peak. However, spurious, nonreplicable peaks were found when using small number trials, artifactual some outlier-like excluded. Further, BOLD-responding thalamus from selected reference function cortex. Thus, unable previously reported results without preselection.

Язык: Английский

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A novel interface for cortical columnar neuromodulation with multipoint infrared neural stimulation

Nature Communications, Год журнала: 2024, Номер 15(1)

Опубликована: Авг. 2, 2024

Abstract Cutting edge advances in electrical visual cortical prosthetics have evoked perception of shapes, motion, and letters the blind. Here, we present an alternative optical approach using pulsed infrared neural stimulation. To interface with dense arrays columns submillimeter spatial precision, both linear array 100-fiber bundle fiber interfaces were devised. We deliver stimulation through these anesthetized cat cortex monitor effects by imaging contralateral cortex. Infrared modulation response to ongoing oriented gratings produce enhanced responses orientation-matched domains suppressed non-matched domains, consistent a known higher order integration mediated callosal inputs. Controls include dynamically applied speeds, directions patterns multipoint This provides groundwork for distinct type prosthetic targeted maps columns.

Язык: Английский

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Systematic evaluation of adhesives for implant fixation in multimodal functional brain MRI

Magnetic Resonance Materials in Physics Biology and Medicine, Год журнала: 2025, Номер unknown

Опубликована: Янв. 15, 2025

Invasive multimodal fMRI in rodents is often compromised by susceptibility artifacts from adhesives used to secure cranial implants. We hypothesized that adhesive type, shape, and field strength significantly affect artifacts, systematically evaluated various adhesives. Thirty-one were applied constrained/unconstrained geometries imaged with T2*-weighted EPI at 7.0 9.4 T assess artifact depths. Spherical flat patch shapes, both unconstrained geometries, compared for depth vitro vivo. Adhesion was assessed on post-mortem mouse crania. Finally, an integrative scoring system rated properties, including depth, handling, adhesion strength. Susceptibility two times larger than (p < 0.001), strongest the edges, deeper spherical patches 0.05). Artifact size depended more shape volume after curing type. Our showed resins, bonding agents, acrylics offered best overall while silicones cements less favorable. Adhesive selection requires balancing time, strength, depth. To minimize should be a spread-out, thin layer. supports classification of future classes

Язык: Английский

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Elucidating hemodynamics and neuro-glio-vascular signaling using rodent fMRI

Trends in Neurosciences, Год журнала: 2025, Номер unknown

Опубликована: Янв. 1, 2025

Despite extensive functional mapping studies using rodent magnetic resonance imaging (fMRI), interpreting the fMRI signals in relation to their neuronal origins remains challenging due hemodynamic nature of response. Ultra high-resolution fMRI, beyond merely enhancing spatial specificity, has revealed vessel-specific responses, highlighting distinct contributions intracortical arterioles and venules signals. This 'single-vessel' approach shifts paradigm enabling its integration with other neuroimaging modalities investigate neuro-glio-vascular (NGV) signaling underlying a variety brain dynamics. Here, we review emerging trend combining multimodal opto/chemogenetic neuromodulation genetically encoded biosensors for cellular circuit-specific recording, offering unprecedented opportunities cross-scale dynamic models.

Язык: Английский

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Selective activation of mesoscale functional circuits via multichannel infrared stimulation of cortical columns in ultra-high-field 7T MRI

Cell Reports Methods, Год журнала: 2025, Номер 5(1), С. 100961 - 100961

Опубликована: Янв. 1, 2025

Язык: Английский

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Optogenetic stimulation of cell bodies versus axonal terminals generate comparable activity and functional connectivity patterns in the brain

Brain stimulation, Год журнала: 2025, Номер unknown

Опубликована: Март 1, 2025

Optogenetic techniques are often employed to dissect neural pathways with presumed specificity for targeted projections. In this study, we used optogenetic fMRI investigate the effective landscape of stimulating cell bodies versus one its projection terminals. Specifically, selected a long-range unidirectional from ventral subiculum (vSUB) nucleus accumbens shell (NAcSh) and placed two fibers-one at vSUB other terminals in NAcSh. Contrary conventional view that terminal stimulation confines activity feedforward stimulated pathway, our findings reveal induces brain connectivity patterns remarkably similar those body stimulation. This observation suggests may induce antidromic activation, leading broader network involvement than previously acknowledged.

Язык: Английский

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A General Framework for Characterizing Optimal Communication in Brain Networks

Опубликована: Март 18, 2025

Communication in brain networks is the foundation of cognitive function and behavior. A multitude evolutionary pressures, including minimization metabolic costs while maximizing communication efficiency, contribute to shaping structure dynamics these networks. However, how efficiency characterized depends on assumed model dynamics. Traditional models include shortest path signaling, random walker navigation, broadcasting, diffusive processes. Yet, a general model-agnostic framework for characterizing optimal neural remains be established.Our study addresses this challenge by assigning through game theory, based combination structural data from human cortical with computational We quantified exact influence exerted each node over every other using an exhaustive multi-site virtual lesioning scheme, creating maps various These descriptions show patterns unfold given network if regions maximize their one another. By comparing large variety models, we found that most closely resembles broadcasting which leverage multiple parallel channels information dissemination. Moreover, influential within cortex are formed its rich-club. exploit topological vantage point across numerous pathways, thereby significantly enhancing effective reach even when anatomical connections weak.Our work provides rigorous versatile reveals features underlying communication.

Язык: Английский

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Multiphoton Neurophotonics: Recent Advances in Imaging and Manipulating Neuronal Circuits

ACS Photonics, Год журнала: 2025, Номер unknown

Опубликована: Апрель 4, 2025

Язык: Английский

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Targeted gene transfer into developmentally defined cell populations of the primate brain

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown

Опубликована: Апрель 17, 2025

Abstract The primate brain possesses unique physiological and developmental features whose systematic investigation is hampered by a paucity of transgenic germline models tools. Here, we present minimally invasive method to introduce transgenes widely across the cerebral cortex using ultrasound-guided fetal intracerebroventricular viral injections (FIVI). This technique enables rapid-onset long-lasting transgene expression following delivery recombinant adeno-associated viruses (rAAVs). By adjusting gestational timing injections, serotypes, transcriptional regulatory elements, rAAV FIVI allows for targeting specific cell populations. We demonstrate versatility this through restricted laminar in cortex, Cre-dependent neurons, CRISPR-based gene editing, labeling peripheral somatosensory retinal pathways. mimicking key desirable models, efficient targeted transfer into opens new avenues experimental translational neuroscience lifespan.

Язык: Английский

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