bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2023,
Номер
unknown
Опубликована: Ноя. 7, 2023
ABSTRACT
Age-related
alterations
in
the
immune
system
are
starting
to
emerge
as
key
contributors
impairments
found
aged
organs.
A
decline
regenerative
capacity
is
a
hallmark
of
tissue
aging,
however
contribution
aging
failure
just
be
explored.
Here,
we
applied
strategy
combining
single-cell
RNA-sequencing
with
flow
cytometry
and
functional
assays
perform
complete
analysis
environment
regenerating
skeletal
muscle,
time
single
cell
resolution.
Our
results
identified
previously
undescribed
types
muscle
revealed
an
unanticipated
complexity
heterogeneity
populations,
that
have
been
regarded
homogeneous.
Furthermore,
uncovered
profound
remodeling
both
myeloid
lymphoid
compartments
aging.
These
discoveries
challenge
established
notions
on
regulation
regeneration,
providing
new
set
potential
targets
improve
health
Frontiers in Immunology,
Год журнала:
2024,
Номер
15
Опубликована: Авг. 29, 2024
Traumatic
spinal
cord
injury
(tSCI)
is
a
severe
to
the
central
nervous
system
that
categorized
into
primary
and
secondary
injuries.
Among
them,
local
microenvironmental
imbalance
in
caused
by
includes
accumulation
of
cytokines
chemokines,
reduced
angiogenesis,
dysregulation
cellular
energy
metabolism,
dysfunction
immune
cells
at
site
injury,
which
severely
impedes
neurological
recovery
from
(SCI).
In
recent
years,
single-cell
techniques
have
revealed
heterogeneity
multiple
genomic,
transcriptomic,
proteomic,
metabolomic
levels
after
tSCI,
further
deepening
our
understanding
mechanisms
underlying
tSCI.
However,
spatial
information
about
tSCI
microenvironment,
such
as
cell
location
cell-cell
interactions,
lost
these
approaches.
The
application
multi-omics
technology
can
solve
this
problem
combining
data
obtained
immunohistochemistry
multiparametric
analysis
reveal
changes
microenvironment
different
times
SCI.
review,
we
systematically
review
progress
study
SCI,
including
discuss
potential
future
therapeutic
strategies.
Journal of Neuroscience Research,
Год журнала:
2023,
Номер
102(1)
Опубликована: Ноя. 10, 2023
Abstract
The
central
nervous
system
has
long
been
thought
to
lack
a
clearance
similar
the
peripheral
lymphatic
system.
Therefore,
of
metabolic
waste
in
subject
great
interest
neuroscience.
Recently,
cerebral
drainage
system,
including
parenchymal
and
meningeal
network,
attracted
considerable
attention.
It
extensively
studied
various
neurological
disorders.
Solute
accumulation
neuroinflammation
after
epilepsy
impair
blood–brain
barrier,
affecting
exchange
between
cerebrospinal
fluid
interstitial
fluid.
Restoring
their
normal
function
may
improve
prognosis
epilepsy.
However,
few
studies
have
focused
on
providing
comprehensive
overview
brain
its
significance
this
review
addressed
structural
composition,
functions,
methods
used
assess
as
well
neglected
association
with
epilepsy,
provided
theoretical
basis
for
therapeutic
approaches
Neuroscience,
Год журнала:
2024,
Номер
549, С. 84 - 91
Опубликована: Март 8, 2024
We
aimed
to
evaluate
the
role
of
spinal
lymphatic
system
in
cord
injury
and
whether
it
has
an
impact
on
recovery
after
injury.
Flow
cytometry
was
used
changes
number
microvesicles
Evans
blue
extravasation
function
system.
immunofluorescence
were
permeability
blood
barrier.
The
edema
evaluated
by
dry
wet
weight.Terminal
deoxynucleotidyl
transferase
dUTP
nick-end
labeling
(TUNEL)
assay
apoptosis
Nuclear
factor-kappa
B
pathway
detected
Western
blot.
Behavioral
tests
limb
function.
Microvesicles
released
can
enter
thoracic
duct
then
through
lymph
around
spine.
After
ligation
duct,
aggravate
neuropathological
manifestations
potential
mechanism
may
involve
nuclear
pathway.
Aging and Disease,
Год журнала:
2024,
Номер
unknown, С. 0 - 0
Опубликована: Янв. 1, 2024
Senescence
is
a
cellular
state
characterized
by
an
irreversible
halt
in
the
cell
cycle,
accompanied
alterations
morphology,
function,
and
secretion.
Senescent
cells
release
plethora
of
inflammatory
growth
factors,
extracellular
matrix
proteins,
other
bioactive
substances,
collectively
known
as
senescence-associated
secretory
phenotype
(SASP).
These
excreted
substances
serve
crucial
mediators
senescent
tissues,
while
secretion
SASP
neurons
glial
central
nervous
system
modulates
activity
immune
cells.
also
influence
physiological
activities
various
system.
Further,
interaction
between
senescence
regulation
affects
pathological
processes
Herein,
we
explore
role
underlying
embryonic
development,
aging,
degeneration,
injury
system,
through
response.
elucidate
proposing
new
theoretical
foundation
for
treating
diseases.
Advanced Materials,
Год журнала:
2024,
Номер
unknown
Опубликована: Окт. 31, 2024
Abstract
Despite
various
biomaterial‐based
strategies
are
tried
in
spinal
cord
injury
(SCI),
developing
safe
and
effective
microinvasive
pharmacotherapy
is
still
an
unmet
clinical
need.
Stimuli‐responsive
nanomedicine
has
emerged
as
a
promising
non‐invasion
technology,
which
enhances
drug
delivery
promotes
functional
recovery
following
SCI.
Considering
the
multiple
progressive
pathological
events
blood
barrier
(BSCB)
associating
SCI,
microenvironment
self‐adaptive
nanoparticle
(custom‐designed
with
rabies
virus
glycoprotein
29‐RVG29
hyaluronic
acid‐HA,
RHNP)
capable
of
consistently
crossing
BSCB
selectively
targeting
inflammatory
cells
neural
based
on
different
stages
SCI
developed.
The
data
indicated
that
RHNP
can
effectively
traverse
through
RVG29,
adaptively
modulate
cellular
internalization
by
exposing
either
HA
or
RVG29
diselenide
bonds,
depending
reactive
oxygen
species
(ROS)
signals.
Furthermore,
curcumin
loaded
into
(RHNP‐Cur)
to
improve
motor
function
coordination
hind‐limbs
traumatic
mouse
model.
This
study
finds
RHNP‐Cur
exhibited
inhibitory
effects
cascade
originating
from
M1
microglia/macrophages
neurotoxic
astrocytes,
protected
inflammation‐induced
apoptosis
during
nerve
regeneration.
Collectively,
work
provides
enables
efficient
treatment
The Journal of Experimental Medicine,
Год журнала:
2024,
Номер
222(2)
Опубликована: Дек. 24, 2024
Dysfunctional
lymphatic
drainage
from
the
central
nervous
system
(CNS)
has
been
linked
to
neuroinflammatory
and
neurodegenerative
disorders,
but
our
understanding
of
contribution
CNS
fluid
autoregulation
remains
limited.
Here,
we
studied
forces
that
drive
outflow
cerebrospinal
(CSF)
into
deep
superficial
cervical
lymph
nodes
(dcLN
scLN)
tested
how
blockade
networks
affects
homeostasis.
Outflow
dcLN
occurred
spontaneously
in
absence
pumping
was
coupled
intracranial
pressure
(ICP),
whereas
scLN
driven
by
pumping.
Impaired
led
elevated
CSF
resistance
delayed
CSF-to-blood
efflux
despite
recruitment
nasal-to-scLN
pathway.
Fluid
regulation
better
compensated
after
obstruction.
The
pathway
exhibited
steady,
consistent
across
conditions,
while
dynamically
activated
mitigate
perturbances.
These
findings
highlight
complex
physiology
homeostasis
lay
groundwork
for
future
studies
aimed
at
assessing
modulating
function.
Neural Regeneration Research,
Год журнала:
2024,
Номер
20(12), С. 3606 - 3619
Опубликована: Июль 29, 2024
JOURNAL/nrgr/04.03/01300535-202512000-00029/figure1/v/2025-01-31T122243Z/r/image-tiff
Secondary
injury
following
spinal
cord
is
primarily
characterized
by
a
complex
inflammatory
response,
with
resident
microglia
and
infiltrating
macrophages
playing
pivotal
roles.
While
previous
studies
have
grouped
these
two
cell
types
together
based
on
similarities
in
structure
function,
an
increasing
number
of
demonstrated
that
exhibit
differences
function
different
effects
disease
processes.
In
this
study,
we
used
single-cell
RNA
sequencing
spatial
transcriptomics
to
identify
the
distinct
evolutionary
paths
injury.
Our
results
showed
were
activated
pro-inflammatory
phenotype
immediately
after
injury,
gradually
transforming
anti-inflammatory
steady
state
as
progressed.
Regarding
macrophages,
our
findings
highlighted
abundant
communication
other
cells,
including
fibroblasts
neurons.
Both
neuroprotective
also
identified;
effect
may
be
related
integrin
β2
(
Itgb2
)
oncostatin
M
pathway.
These
validated
vivo
experiments.
This
research
underscores
cellular
dynamics
offer
new
perspectives
mechanisms
potential
therapeutic
targets.
Advanced Science,
Год журнала:
2024,
Номер
unknown
Опубликована: Сен. 28, 2024
Abstract
Reconstruction
of
the
neurovascular
unit
is
essential
for
repair
spinal
cord
injury
(SCI).
Nonetheless,
detailed
documentation
specific
vascular
changes
following
SCI
and
targeted
interventions
treatment
remains
limited.
This
study
demonstrates
that
traumatic
pathological
remodeling
occurs
during
chronic
phase
injury,
characterized
by
enlarged
vessel
diameter,
disruption
blood‐spinal
barrier,
endothelial‐to‐mesenchymal
transition
(EndoMT),
heightened
extracellular
matrix
deposition.
After
SCI,
osteopontin
(OPN),
a
critical
factor
secreted
immune
cells,
indispensable
early
regeneration
but
also
contributes
to
remodeling.
work
further
elucidates
mechanism
which
OPN
influences
microvascular
endothelial
involving
Akt‐mediated
Foxo1
phosphorylation.
process
facilitates
extranuclear
transport
decreases
Smad7
expression,
leading
excessive
activation
TGF‐β
signaling
pathway,
ultimately
results
in
EndoMT
fibrosis.
Targeted
inhibition
phosphorylation
through
an
endothelium‐specific
aptamer‐liposome
small
molecule
delivery
system
significantly
mitigates
remodeling,
thereby
enhancing
axon
neurological
function
recovery
SCI.
The
findings
offer
novel
perspective
drug
therapies
aimed
at
specifically
targeting
vasculature
after
