bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Ноя. 8, 2024
Abstract
Although
many
methods
for
automated
fluorescent-labeled
cell
detection
have
been
proposed,
not
all
of
them
assume
a
highly
inhomogeneous
background
arising
from
complex
biological
structures.
Here,
we
propose
an
algorithm
that
accounts
and
subtracts
the
by
avoiding
high-intensity
pixels
in
blur
filtering
calculation.
Cells
were
detected
intensity
thresholding
background-subtracted
image,
algorithm’s
performance
was
tested
on
NeuN-
c-Fos-stained
images
mouse
prefrontal
cortex
hippocampal
dentate
gyrus.
In
addition,
applications
c-Fos
positive
counting
quantification
expression
level
double-labeled
cells
demonstrated.
Our
method
after
assumption
(ADABA)
offers
advantage
high-throughput
unbiased
analysis
regions
with
structures
produce
background.
Highlights
-
We
proposed
to
subtract
pattern.
(79/85)
automatically
image.
(71/85)
The
results
corresponded
manual
detection.
(73/85)
Detection
IEG
overlapping
neural
marker
(85/85)
Criminal Justice Ethics,
Год журнала:
2024,
Номер
43(2), С. 146 - 193
Опубликована: Май 3, 2024
In
criminal
proceedings,
offenders
are
sentenced
based
on
doctrines
of
culpability
and
punishment
that
theorize
why
they
guilty
should
be
punished.
Throughout
human
history,
these
have
largely
been
grounded
in
legal-policy
constructions
around
retribution,
safety,
deterrence,
closure,
mostly
derived
from
folk
psychology,
natural
philosophy,
sociocultural
expectations,
public-order
narratives,
common
sense.
On
premises,
justice
systems
long
designed
to
account
for
crimes
their
underlying
intent,
with
experience
probabilistic
assumptions
shaping
theoretical
discourses
the
nature
offenders'
punishability.
As
scientific
discoveries,
inventions,
methodologies
progressively
developed
refine
such
displace
long-held
assumptions,
courtrooms
increasingly
witnessed
counsels
judges
relying
evidence
submit,
dispute,
or
validate
claims.
For
instance,
over
last
century,
selectively
admitted
neuroscientific
models,
exams,
insights
claiming
revolutionize
our
understanding
who
is
culpable
deserving
punishment.
Most
recently,
advancements
epigenetics
promised
even
more
profound
challenges
long-standing
law
doctrines.
This
article
examines
reasons
reversibility
inheritability
epigenetic
markers
might
warrant
revising
concludes
findings
not
yet
robust
enough
justify
revisions.
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Ноя. 8, 2024
Abstract
Although
many
methods
for
automated
fluorescent-labeled
cell
detection
have
been
proposed,
not
all
of
them
assume
a
highly
inhomogeneous
background
arising
from
complex
biological
structures.
Here,
we
propose
an
algorithm
that
accounts
and
subtracts
the
by
avoiding
high-intensity
pixels
in
blur
filtering
calculation.
Cells
were
detected
intensity
thresholding
background-subtracted
image,
algorithm’s
performance
was
tested
on
NeuN-
c-Fos-stained
images
mouse
prefrontal
cortex
hippocampal
dentate
gyrus.
In
addition,
applications
c-Fos
positive
counting
quantification
expression
level
double-labeled
cells
demonstrated.
Our
method
after
assumption
(ADABA)
offers
advantage
high-throughput
unbiased
analysis
regions
with
structures
produce
background.
Highlights
-
We
proposed
to
subtract
pattern.
(79/85)
automatically
image.
(71/85)
The
results
corresponded
manual
detection.
(73/85)
Detection
IEG
overlapping
neural
marker
(85/85)
