Spatially specific, closed-loop infrared thalamocortical deep brain stimulation DOI Creative Commons
Brandon S. Coventry, Georgia L. Lawlor,

Christina B Bagnati

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Окт. 6, 2023

Deep brain stimulation (DBS) is a powerful tool for the treatment of circuitopathy-related neurological and psychiatric diseases disorders such as Parkinson's disease obsessive-compulsive disorder, well critical research perturbing neural circuits exploring neuroprostheses. Electrically-mediated DBS, however, limited by spread stimulus currents into tissue unrelated to course treatment, potentially causing undesirable patient side effects. In this work, we utilize infrared (INS), an optical neuromodulation technique that uses near mid-infrared light drive graded excitatory inhibitory responses in nerves neurons, facilitate spatially constrained DBS paradigm. INS has been shown provide cortical neurons and, unlike other techniques, does not require genetic modification target. We show produces graded, biophysically relevant single-unit with robust information transfer thalamocortical circuits. Importantly, activation from thalamic more response profiles than conventional electrical stimulation. Owing observed spatial precision INS, used deep reinforcement learning closed-loop control circuits, creating real-time representations stimulus-response dynamics while driving precise firing patterns. Our data suggest can serve targeted dynamic paradigm both open DBS.

Язык: Английский

Integrated Microprism and Microelectrode Array for Simultaneous Electrophysiology and Two‐Photon Imaging across All Cortical Layers DOI Creative Commons
Qianru Yang, Bingchen Wu, Elisa Castagnola

и другие.

Advanced Healthcare Materials, Год журнала: 2024, Номер unknown

Опубликована: Апрель 2, 2024

Cerebral neural electronics play a crucial role in neuroscience research with increasing translational applications such as brain-computer interfaces for sensory input and motor output restoration. While widely utilized decades, the understanding of cellular mechanisms underlying this technology remains limited. Although two-photon microscopy (TPM) has shown great promise imaging superficial electrodes, its application to deep-penetrating electrodes is technically difficult. Here, novel device integrating transparent microelectrode arrays glass microprisms, enabling electrophysiology recording stimulation alongside TPM across all cortical layers vertical plane, introduced. Tested Thy1-GCaMP6 mice over 4 months, integrated demonstrates capability multisite electrophysiological recording/stimulation simultaneous calcium imaging. As proof concept, impact microstimulation amplitude, frequency, depth on activation patterns investigated using setup. With future improvements material stability single unit yield, multimodal tool greatly expands optical from brain entire column, opening new avenues neurotechnology development.

Язык: Английский

Процитировано

2
Protocol for recording neural activity evoked by electrical stimulation in mice using two-photon calcium imaging DOI Creative Commons
Seungbin Park,

Megan Lipton,

Yujiao Jennifer Sun

и другие.

STAR Protocols, Год журнала: 2024, Номер 5(2), С. 103027 - 103027

Опубликована: Апрель 27, 2024

Electrical stimulation provides a clinically viable approach for treating neurological disorders. Here, we present protocol recording neural activity evoked by electrical in mice using two-photon calcium imaging. We detail steps chronically implanting head fixation bar, stimulating electrode, and glass imaging window. additionally describe the procedures viral injections awake head-fixed recordings. For complete details on use execution of this protocol, please refer to Dadarlat et al.

Язык: Английский

Процитировано

0
Spike-wave discharges during low-current thalamic deep brain stimulation in mice DOI Open Access
Francisco Flores, Isabella Dalla Betta, John Tauber

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Май 12, 2024

Deep brain stimulation of central thalamus (CT-DBS) has potential for modulating states consciousness, but it can also trigger spike-wave discharges (SWDs).

Язык: Английский

Процитировано

0
EFMouse: a Matlab toolbox to model electric fields in the mouse brain DOI Creative Commons
Rubén Sánchez-Romero,

Sibel Akyüz,

Bart Krekelberg

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Июль 26, 2024

Abstract Compared to the rapidly growing literature on transcranial electrical stimulation (tES) in humans, research into mechanisms underlying neuromodulation by tES using in-vivo animal models is but still relatively rare. Such research, however, key overcoming experimental limitations humans and essential build a detailed understanding of consequences that can ultimately lead development targeted effective therapeutic applications noninvasive brain stimulation. The sheer difference scale geometry between human contributes complexity designing interpreting studies. Here we extend previous approaches model intracranial electric fields generate predictions be tested with recordings. Although toolbox has general applicability could used predict for any study mice, illustrate its usage comparing high-density multi-electrode montage more traditional two electrode montage. Our simulations show both montages produce strong focal homogeneous areas. However, produces field perpendicular visual cortical surface, which expected result larger changes neuronal excitability. Highlights - EFMouse novel, open-source, Matlab-based simulator mouse brain. quantifies focality homogeneity regions Allen Mouse Brain Atlas. Focal produced or five montages. A lumbar return generates surface.

Язык: Английский

Процитировано

0
Electrographic seizures during low-current thalamic deep brain stimulation in mice DOI Creative Commons
Francisco Flores, Isabella Dalla Betta, John Tauber

и другие.

Brain stimulation, Год журнала: 2024, Номер 17(5), С. 975 - 979

Опубликована: Авг. 10, 2024

Язык: Английский

Процитировано

0
Inkjet-printed transparent electrodes for electrical brain stimulation DOI Creative Commons

Rita Matta,

Davide Reato,

Alberto Lombardini

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Сен. 10, 2024

ABSTRACT Electrical stimulation is a powerful tool for investigating and modulating brain activity, as well treating neurological disorders. However, understanding the precise effects of electrical on neural activity has been hindered by limitations in recording neuronal responses near stimulating electrode, such artifacts electrophysiology or obstruction field view imaging. In this study, we introduce novel device fabricated from conductive polymers that transparent therefore compatible with optical imaging techniques. The manufactured using combination microfabrication inkjet printing techniques flexible, allowing better adherence to brain’s natural curvature. We characterized properties electrode evaluated its performance an anesthetized mouse. Furthermore, combined experimental data finite-element model in-vivo setup estimate maximum electric highly can generate mouse brain. Our findings indicate high 300 V/m, demonstrating potential studying manipulating range relevant human applications. Overall, work presents promising approach developing versatile new tools apply study stimulation.

Язык: Английский

Процитировано

0
Microscopic deconstruction of cortical circuit stimulation by transcranial ultrasound DOI Open Access
Théo Lemaire, Yi Yuan, Celia Gellman

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Окт. 12, 2024

ABSTRACT Transcranial Ultrasound Stimulation (TUS) can noninvasively and reversibly perturb neuronal activity, but the mechanisms by which ultrasound engages brain circuits to induce functional effects remain unclear. To elucidate these interactions, we applied TUS cortex of awake mice concurrently monitored local neural activity at acoustic focus with two-photon calcium imaging. We show that evokes highly focal responses in three canonical populations, cell-type-specific dose dependencies. Through independent parametric variations, demonstrate evoked collectively scale time-average intensity stimulus. Finally, using computational unmixing propose a physiologically realistic cortical circuit model predicts TUS-evoked as result both direct network interactions. Our results provide first evidence TUS’s on shed light complex underlying effects, paving way for deployment clinical settings.

Язык: Английский

Процитировано

0
A Model-Driven Meta-Analysis Supports the Emerging Consensus View that Inhibitory Neurons Dominate BOLD-fMRI Responses DOI Creative Commons
Nicolas Sundqvist, Henrik Podéus, Sebastian Sten

и другие.

Опубликована: Окт. 17, 2024

Functional magnetic resonance imaging (fMRI) is a pivotal tool for mapping neuronal activity in the brain. Traditionally, observed hemodynamic changes are assumed to reflect of most common type: excitatory neurons. In contrast, recent experiments, using optogenetic techniques, suggest that fMRI-signal instead reflects inhibitory interneurons. However, these data paint complex picture, with numerous regulatory interactions, and where different experiments display many qualitative differences. It therefore not trivial how quantify relative contributions cell types combine all observations into unified theory. To address this, we present new model-driven meta-analysis, which provides quantitative explanation data. This analysis allows quantification contribution types: BOLD-signal from cells <20 % 50-80 comes Our also mechanistic experiment-to-experiment differences, e.g. biphasic vascular response dependent on stimulation intensities an emerging secondary post-stimulation peak during longer stimulations. summary, our study new, consensus-view supporting larger role interneurons fMRI.

Язык: Английский

Процитировано

0
Spatially specific, closed-loop infrared thalamocortical deep brain stimulation DOI Creative Commons
Brandon S. Coventry, Georgia L. Lawlor,

Christina B Bagnati

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Окт. 6, 2023

Deep brain stimulation (DBS) is a powerful tool for the treatment of circuitopathy-related neurological and psychiatric diseases disorders such as Parkinson's disease obsessive-compulsive disorder, well critical research perturbing neural circuits exploring neuroprostheses. Electrically-mediated DBS, however, limited by spread stimulus currents into tissue unrelated to course treatment, potentially causing undesirable patient side effects. In this work, we utilize infrared (INS), an optical neuromodulation technique that uses near mid-infrared light drive graded excitatory inhibitory responses in nerves neurons, facilitate spatially constrained DBS paradigm. INS has been shown provide cortical neurons and, unlike other techniques, does not require genetic modification target. We show produces graded, biophysically relevant single-unit with robust information transfer thalamocortical circuits. Importantly, activation from thalamic more response profiles than conventional electrical stimulation. Owing observed spatial precision INS, used deep reinforcement learning closed-loop control circuits, creating real-time representations stimulus-response dynamics while driving precise firing patterns. Our data suggest can serve targeted dynamic paradigm both open DBS.

Язык: Английский

Процитировано

0