A polymeric, PDMS-based large-scale skull replacement suitable for optical and mechanical access for long-term neuronal imaging, electrophysiology, and optogenetics DOI Creative Commons
Kengo Takahashi, Gerjan Huis in ’t Veld, Davide Benedetti

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown

Опубликована: Апрель 20, 2025

Summary Many techniques to record and manipulate neuronal activity across large portions of the vertebrate brain, such as widefield two-photon calcium imaging, electrophysiology, optogenetics, are now available. However, few effective approaches enable both optical mechanical access brain. In this work, we offer an in-depth guide for synthesizing, implanting, using polydimethylsiloxane (PDMS) windows skull replacements chronic imaging. Furthermore, provide instructions perform viral injections multi-site silicon probe implantation. Graphical abstract

Язык: Английский

Neuropixels Opto: Combining high-resolution electrophysiology and optogenetics DOI Creative Commons
Anna Lakunina, Karolina Socha, Alexander Ladd

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown

Опубликована: Фев. 6, 2025

High-resolution extracellular electrophysiology is the gold standard for recording spikes from distributed neural populations, and especially powerful when combined with optogenetics manipulation of specific cell types high temporal resolution. We integrated these approaches into prototype Neuropixels Opto probes, which combine electronic photonic circuits. These devices pack 960 electrical sites two sets 14 light emitters onto a 1 cm shank, allowing spatially addressable optogenetic stimulation blue red light. In mouse cortex, probes delivered high-quality recordings together optogenetics, differentially activating or silencing neurons at distinct cortical depths. striatum other deep structures, efficient optotagging, facilitating identification in parallel. represent an unprecedented tool recording, identifying, manipulating neuronal populations.

Язык: Английский

Процитировано

4

Foundry-fabricated dual-color nanophotonic neural probes for photostimulation and electrophysiological recording DOI Creative Commons
David A. Roszko, Fu‐Der Chen,

John N. Straguzzi

и другие.

Neurophotonics, Год журнала: 2025, Номер 12(02)

Опубликована: Март 28, 2025

Compact tools capable of delivering multicolor optogenetic stimulation to deep tissue targets with sufficient span, spatiotemporal resolution, and optical power remain challenging realize. Here, we demonstrate foundry-fabricated nanophotonic neural probes for blue red photostimulation electrophysiological recording, which use a combination spatial multiplexing on-shank wavelength demultiplexing increase the number emitters. We silicon (Si) photonic 26 channels recording sites, were fabricated on 200-mm diameter wafers at commercial Si photonics foundry. Each channel consists an demultiplexer separate grating coupler emitters light, total 52 evaluate probe functionality through bench measurements in vivo experiments by photostimulating 16 available emitter pairs. report electrode impedances, transmission, beam profiles. validated packaged mice sensitive or photostimulation. Our demonstrates dense dual-color integration single shank targeted Given its two emission wavelengths, high density, long site this will facilitate involving bidirectional circuit manipulations across both shallow structures simultaneously.

Язык: Английский

Процитировано

0

Computer vision–guided rapid and precise automated cranial microsurgeries in mice DOI
Zahra S. Navabi,

Ryan M. Peters,

Beatrice R. Gulner

и другие.

Science Advances, Год журнала: 2025, Номер 11(15)

Опубликована: Апрель 9, 2025

A common procedure that allows interfacing with the brain is cranial microsurgery, wherein small to large craniotomies are performed on overlying skull for insertion of neural interfaces or implantation optically clear windows long-term observation. Performing requires skill, time, and precision avoid damaging dura. Here, we present a computer vision–guided craniotomy robot (CV-Craniobot) uses machine learning accurately estimate dorsal anatomy from optical coherence tomography images. Instantaneous information morphology used by robotic mill rapidly precisely remove desired location. We show CV-Craniobot can perform (2- 4-millimeter diameter) near 100% success rates within 2 minutes encompassing most cortex in less than 10 minutes. Thus, enables rapid precise craniotomies, reducing surgery time compared human practitioners eliminating need long training.

Язык: Английский

Процитировано

0

A polymeric, PDMS-based large-scale skull replacement suitable for optical and mechanical access for long-term neuronal imaging, electrophysiology, and optogenetics DOI Creative Commons
Kengo Takahashi, Gerjan Huis in ’t Veld, Davide Benedetti

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown

Опубликована: Апрель 20, 2025

Summary Many techniques to record and manipulate neuronal activity across large portions of the vertebrate brain, such as widefield two-photon calcium imaging, electrophysiology, optogenetics, are now available. However, few effective approaches enable both optical mechanical access brain. In this work, we offer an in-depth guide for synthesizing, implanting, using polydimethylsiloxane (PDMS) windows skull replacements chronic imaging. Furthermore, provide instructions perform viral injections multi-site silicon probe implantation. Graphical abstract

Язык: Английский

Процитировано

0