Neuropixels Opto: Combining high-resolution electrophysiology and optogenetics
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2025,
Номер
unknown
Опубликована: Фев. 6, 2025
High-resolution
extracellular
electrophysiology
is
the
gold
standard
for
recording
spikes
from
distributed
neural
populations,
and
especially
powerful
when
combined
with
optogenetics
manipulation
of
specific
cell
types
high
temporal
resolution.
We
integrated
these
approaches
into
prototype
Neuropixels
Opto
probes,
which
combine
electronic
photonic
circuits.
These
devices
pack
960
electrical
sites
two
sets
14
light
emitters
onto
a
1
cm
shank,
allowing
spatially
addressable
optogenetic
stimulation
blue
red
light.
In
mouse
cortex,
probes
delivered
high-quality
recordings
together
optogenetics,
differentially
activating
or
silencing
neurons
at
distinct
cortical
depths.
striatum
other
deep
structures,
efficient
optotagging,
facilitating
identification
in
parallel.
represent
an
unprecedented
tool
recording,
identifying,
manipulating
neuronal
populations.
Язык: Английский
Foundry-fabricated dual-color nanophotonic neural probes for photostimulation and electrophysiological recording
Neurophotonics,
Год журнала:
2025,
Номер
12(02)
Опубликована: Март 28, 2025
Compact
tools
capable
of
delivering
multicolor
optogenetic
stimulation
to
deep
tissue
targets
with
sufficient
span,
spatiotemporal
resolution,
and
optical
power
remain
challenging
realize.
Here,
we
demonstrate
foundry-fabricated
nanophotonic
neural
probes
for
blue
red
photostimulation
electrophysiological
recording,
which
use
a
combination
spatial
multiplexing
on-shank
wavelength
demultiplexing
increase
the
number
emitters.
We
silicon
(Si)
photonic
26
channels
recording
sites,
were
fabricated
on
200-mm
diameter
wafers
at
commercial
Si
photonics
foundry.
Each
channel
consists
an
demultiplexer
separate
grating
coupler
emitters
light,
total
52
evaluate
probe
functionality
through
bench
measurements
in
vivo
experiments
by
photostimulating
16
available
emitter
pairs.
report
electrode
impedances,
transmission,
beam
profiles.
validated
packaged
mice
sensitive
or
photostimulation.
Our
demonstrates
dense
dual-color
integration
single
shank
targeted
Given
its
two
emission
wavelengths,
high
density,
long
site
this
will
facilitate
involving
bidirectional
circuit
manipulations
across
both
shallow
structures
simultaneously.
Язык: Английский
Computer vision–guided rapid and precise automated cranial microsurgeries in mice
Science Advances,
Год журнала:
2025,
Номер
11(15)
Опубликована: Апрель 9, 2025
A
common
procedure
that
allows
interfacing
with
the
brain
is
cranial
microsurgery,
wherein
small
to
large
craniotomies
are
performed
on
overlying
skull
for
insertion
of
neural
interfaces
or
implantation
optically
clear
windows
long-term
observation.
Performing
requires
skill,
time,
and
precision
avoid
damaging
dura.
Here,
we
present
a
computer
vision–guided
craniotomy
robot
(CV-Craniobot)
uses
machine
learning
accurately
estimate
dorsal
anatomy
from
optical
coherence
tomography
images.
Instantaneous
information
morphology
used
by
robotic
mill
rapidly
precisely
remove
desired
location.
We
show
CV-Craniobot
can
perform
(2-
4-millimeter
diameter)
near
100%
success
rates
within
2
minutes
encompassing
most
cortex
in
less
than
10
minutes.
Thus,
enables
rapid
precise
craniotomies,
reducing
surgery
time
compared
human
practitioners
eliminating
need
long
training.
Язык: Английский
A polymeric, PDMS-based large-scale skull replacement suitable for optical and mechanical access for long-term neuronal imaging, electrophysiology, and optogenetics
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2025,
Номер
unknown
Опубликована: Апрель 20, 2025
Summary
Many
techniques
to
record
and
manipulate
neuronal
activity
across
large
portions
of
the
vertebrate
brain,
such
as
widefield
two-photon
calcium
imaging,
electrophysiology,
optogenetics,
are
now
available.
However,
few
effective
approaches
enable
both
optical
mechanical
access
brain.
In
this
work,
we
offer
an
in-depth
guide
for
synthesizing,
implanting,
using
polydimethylsiloxane
(PDMS)
windows
skull
replacements
chronic
imaging.
Furthermore,
provide
instructions
perform
viral
injections
multi-site
silicon
probe
implantation.
Graphical
abstract
Язык: Английский