bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2023,
Номер
unknown
Опубликована: Ноя. 5, 2023
Magnetic
resonance
spectroscopy
(MRS)
is
widely
used
to
estimate
concentrations
of
glutamate
and
γ-aminobutyric
acid
(GABA)
in
specific
regions
the
living
human
brain.
As
cytoarchitectural
properties
differ
across
brain,
interpreting
these
measurements
can
be
assisted
by
having
knowledge
such
for
MRS
region(s)
studied.
In
particular,
some
likely
local
neurotransmitter
receptor
patterns
potentially
give
insights
into
mechanistic
environment
GABAand
glutamatergic
neurons
are
functioning
in.
This
may
particular
utility
when
comparing
two
or
more
regions,
given
that
populations
substantially
them.
At
same
time,
studying
data
from
multiple
participants
timepoints,
homogeneity
sample
becomes
relevant,
as
taken
areas
with
different
cytoarchitecture
difficult
compare.
To
provide
user-defined
regions-of-interest,
we
produced
an
easy
use
tool
InSpectro-Gadget,
interfaces
mRNA
expression
information
Allen
Human
Brain
Atlas.
Python
allows
users
input
masks
automatically
obtain
a
graphical
overview
population
found
within.
includes
comparison
between
where
relevant.
The
receptors
subunit
genes
featured
include
GABA-
classes,
along
wide
range
neuromodulators.
functionality
explained
here
its
demonstrated
through
set
example
analyses.
available
at
https://github.com/lizmcmanus/Inspectro-Gadget
.
Molecules,
Год журнала:
2025,
Номер
30(2), С. 396 - 396
Опубликована: Янв. 18, 2025
Ratiometric
lanthanide
coordination
polymers
(Ln-CPs)
are
advanced
materials
that
combine
the
unique
optical
properties
of
ions
(e.g.,
Eu3+,
Tb3+,
Ce3+)
with
structural
flexibility
and
tunability
polymers.
These
widely
used
in
biological
chemical
sensing,
environmental
monitoring,
medical
diagnostics
due
to
their
narrow-band
emission,
long
fluorescence
lifetimes,
excellent
resistance
photobleaching.
This
review
focuses
on
composition,
sensing
mechanisms,
applications
ratiometric
Ln-CPs.
The
mechanism
relies
two
distinct
emission
bands,
which
provides
a
self-calibrating,
reliable,
precise
method
for
detection.
relative
intensity
ratio
between
these
bands
varies
concentration
target
analyte,
enabling
real-time
monitoring
minimizing
interference.
approach
is
particularly
suitable
detecting
trace
analytes
use
complex
environments
where
factors
like
background
noise,
temperature
fluctuations,
light
variations
may
affect
results.
Finally,
we
outline
future
research
directions
improving
design
synthesis
Ln-CPs,
such
as
incorporating
long-lifetime
reference
luminescent
molecules,
exploring
near-infrared
systems,
developing
up-conversion
or
two-photon
materials.
Progress
areas
could
significantly
broaden
scope
Ln-CP
applications,
especially
biosensing,
other
fields.
ACS Applied Materials & Interfaces,
Год журнала:
2024,
Номер
16(11), С. 13384 - 13398
Опубликована: Март 8, 2024
Organic
electrochemical
transistors
(OECTs)
employing
conductive
polymers
(CPs)
have
gained
remarkable
prominence
and
undergone
extensive
advancements
in
wearable
implantable
bioelectronic
applications
recent
years.
Among
the
diverse
arrays
of
CPs,
poly(3,4-ethylenedioxythiophene):poly(styrenesulfonate)
(PEDOT:PSS)
is
a
common
choice
for
active-layer
channel
p-type
OECTs,
showing
remarkably
high
transconductance
amplification
signals
biosensing
applications.
This
investigation
focuses
on
novel
engineering
PEDOT:PSS
composite
materials
by
seamlessly
integrating
several
additives,
namely,
dimethyl
sulfoxide
(DMSO),
(3-glycidyloxypropyl)trimethoxysilane
(GOPS),
nonionic
fluorosurfactant
(NIFS),
to
fine-tune
their
electrical
conductivity,
self-healing
capability,
stretchability.
To
elucidate
intricate
influences
DMSO,
GOPS,
NIFS
additives
formation
films,
theoretical
calculations
were
performed,
encompassing
solubility
parameters
surface
energies
constituent
components
NIFS,
PEDOT,
PSS,
PSS-GOPS
polymers.
Furthermore,
we
conducted
comprehensive
array
material
analyses,
which
reveal
intricacies
phase
separation
phenomenon
its
interaction
with
materials'
characteristics.
Our
research
identified
optimal
composition
characterized
outstanding
stretchable
capabilities.
has
proven
be
highly
effective
constructing
an
form
OECT-based
biosensors
fabricated
onto
polydimethylsiloxane
substrates
detecting
dopamine.
Overall,
these
findings
represent
significant
progress
application
films
bioelectronics
pave
way
development
state-of-the-art
technologies.
Biosensors and Bioelectronics,
Год журнала:
2024,
Номер
256, С. 116242 - 116242
Опубликована: Март 29, 2024
Psychiatric
disorders
are
associated
with
serve
disturbances
in
cognition,
emotional
control,
or
behavior
regulation,
yet
few
routine
clinical
tools
available
for
the
real-time
evaluation
and
early-stage
diagnosis
of
mental
health.
Abnormal
levels
biomarkers
might
imply
biological,
neurological,
developmental
dysfunctions
psychiatric
patients.
Exploring
biosensors
that
can
provide
rapid,
in-situ,
monitoring
is
therefore
vital
prevention,
diagnosis,
treatment,
prognosis
disorders.
Recently,
high
sensitivity,
selectivity,
reproducibility
have
been
widely
developed,
which
mainly
based
on
electrochemical
optical
sensing
technologies.
This
review
presented
morbidity,
disability,
mortality,
followed
by
describing
pathophysiology
a
biomarker-implying
manner.
The
latest
developed
detecting
representative
(e.g.,
cortisol,
dopamine,
serotonin)
were
comprehensively
summarized
compared
their
technologies,
applicable
biological
platforms,
integrative
readouts.
These
well-developed
promising
facilitating
utility
commercialization
point-of-care
diagnostics.
It
anticipated
healthcare
be
gradually
improved
multiple
perspectives,
from
innovations
terms
biometric
elements,
transducing
principles,
flexible
readouts,
to
construction
"Big-Data"
networks
used
share
intractable
indicators
cases.
Neural Regeneration Research,
Год журнала:
2024,
Номер
19(12), С. 2649 - 2660
Опубликована: Март 1, 2024
Parkinson’s
disease
is
characterized
by
the
selective
degeneration
of
dopamine
neurons
in
nigrostriatal
pathway
and
deficiency
striatum.
The
precise
reasons
behind
specific
these
remain
largely
elusive.
Genetic
investigations
have
identified
over
20
causative
PARK
genes
90
genomic
risk
loci
associated
with
both
familial
sporadic
disease.
Notably,
several
are
linked
to
synaptic
vesicle
recycling
process,
particularly
clathrin-mediated
endocytosis
pathway.
This
suggests
that
impaired
might
represent
an
early
feature
disease,
followed
axonal
eventual
loss
cell
bodies
midbrain
via
a
“dying
back”
mechanism.
Recently,
new
animal
cellular
models
disease-linked
mutations
affecting
endocytic
been
created
extensively
characterized.
These
faithfully
recapitulate
certain
disease-like
features
at
animal,
circuit,
levels,
exhibit
defects
membrane
trafficking,
further
supporting
findings
from
human
genetics
clinical
studies.
In
this
review,
we
will
first
summarize
molecular
two
clathrin
uncoating
proteins:
auxilin
(
DNAJC6
/
PARK19
)
synaptojanin
1
SYNJ1
PARK20
).
mouse
carrying
gene
phenocopy
each
other
terminal
pathology
display
potent
synergistic
effect.
Subsequently,
delve
into
involvement
endocytosis-related
proteins
(GAK,
endophilin
A1,
SAC2/INPP5F,
synaptotagmin-11),
as
factors
through
genome-wide
association
studies,
pathogenesis.
We
also
explore
direct
or
indirect
roles
some
common
(alpha-synuclein
(PARK1/4),
Parkin
(PARK2),
LRRK2
(PARK8))
trafficking.
Additionally,
discuss
emerging
novel
functions
downstream
traffic
pathways,
autophagy.
Given
dysfunction
considered
event
deeper
understanding
mechanisms
underlying
trafficking
may
unveil
targets
for
diagnosis
development
interventional
therapies
Future
research
should
aim
elucidate
why
generalized
leads
Sensors,
Год журнала:
2025,
Номер
25(6), С. 1729 - 1729
Опубликована: Март 11, 2025
Nanomaterials
and
sensors
play
an
important
role
in
modern
technologies,
including
medical
diagnostics
biochemical
research.
This
work
presents
the
possibility
of
using
o-Phthaldialdehyde
(OPA)
combination
with
3-mercaptopropyltriethoxysilane
(MPTES)
to
develop
a
dopamine-responsive
sensor.
During
experiment,
these
materials
were
used
at
different
pH
ratios
determine
optimal
parameters
for
obtaining
high
fluorescence
intensity
reaction
product.
The
data
obtained
demonstrate
linear
relationship
between
response
(λex/λem
=
340/460
nm)
OPA/MPTES
dopamine
concentration
range
0.1–3.0
µM
8,
detection
limit
was
8.7
nM.
results
confirm
potential
as
sensing
component
dopamine.
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Дек. 23, 2024
Neurochemical
signals
like
dopamine
(DA)
play
a
crucial
role
in
variety
of
brain
functions
through
intricate
interactions
with
other
neuromodulators
and
intracellular
signaling
pathways.
However,
studying
these
complex
networks
has
been
hindered
by
the
challenge
detecting
multiple
neurochemicals
vivo
simultaneously.
To
overcome
this
limitation,
we
developed
single-protein
chemigenetic
DA
sensor,
HaloDA1.0,
which
combines
cpHaloTag-chemical
dye
approach
G
protein-coupled
receptor
activation-based
(GRAB)
strategy,
providing
high
sensitivity
for
DA,
sub-second
response
kinetics,
an
extensive
spectral
range
from
far-red
to
near-infrared.
When
used
together
existing
green
red
fluorescent
neuromodulator
sensors,
Ca2+
indicators,
cAMP
optogenetic
tools,
HaloDA1.0
provides
versatility
multiplex
imaging
cultured
neurons,
slices,
behaving
animals,
facilitating
in-depth
studies
dynamic
neurochemical
networks.
Molecular Physics,
Год журнала:
2024,
Номер
123(4)
Опубликована: Июль 4, 2024
Density
functional
theory
(DFT)
was
employed
to
investigate
the
sensing
behaviour
of
neurotransmitter
dopamine
(DA)
when
interacting
with
OH-functionalised
copper
phthalocyanines
(CuPcs)
and
coadsorbed
iodine
(CuIPc),
both
in
gaseous
aqueous
media.
This
study
revealed
that
CuIPc
demonstrates
a
superior
capacity
for
detecting
molecules
compared
CuPc.
Within
these
complexes,
hydrogen
bonds
coordination
were
observed,
playing
pivotal
role
adsorption
process.
The
enhanced
electrical
conductivity
CuPc
sheets
after
adsorption,
along
high
energy
iodine-coadsorbed
CuPc/DA
underscores
significance
this
context.
Notably,
utilisation
significantly
enhances
response
dopamine.
In
summary,
phthalocyanine
has
emerged
as
promising
material
sensors,
offering
possibilities
further
advancements
field.
Abstract
Dopamine
is
an
important
catecholamine
neurotransmitter
and
its
precise
determination
of
great
significance
in
the
diagnosis
treatment
related
diseases.
This
study
presents
a
high‐performance
enzyme‐free
electrochemical
sensor
for
dopamine
based
on
combination
Prussian
blue
analogues
oxides
with
multi‐walled
carbon
nanotubes
(PBO@CNTs).
The
prepared
PBO@CNTs
exhibited
increased
surface
area
49.79
m
2
/g
abundant
mesoporous
nanostructure,
which
providing
numerous
electroactive
sites.
Under
optimal
conditions,
PBO@CNTs/Au
high
sensitivity
178.34
μA
mM
−1
cm
−2
,
broad
linear
range
between
0.30
μM
to
8
low
detection
limit
0.10
μM.
It
displayed
excellent
selectivity
towards
by
successive
addition
various
interfering
species
through
chronoamperometry
studies.
Besides,
proposed
electrodes
enjoyed
repeatability,
reproducibility
long‐term
stability.
We
also
demonstrated
successful
application
this
serum.
results
indicated
that
dopamine‐selective
sensors
have
potential
clinical
application.