MiR-10b-5p attenuates spinal cord injury and alleviates LPS-induced PC12 cells injury by inhibiting TGF-β1 decay and activating TGF-β1/Smad3 pathway through PTBP1 DOI Creative Commons

Huandong Liu,

Chong Liang,

Hongfei Liu

и другие.

European journal of medical research, Год журнала: 2024, Номер 29(1)

Опубликована: Ноя. 19, 2024

Spinal cord injury (SCI) is a debilitating condition characterized by significant sensory, motor, and autonomic dysfunctions, leading to severe physical, psychological, financial burdens. The current therapeutic approaches for SCI show limited effectiveness, highlighting the urgent need innovative treatments. MicroRNAs (miRNAs) like miR-10b-5p are known play pivotal roles in gene expression regulation have been implicated various neurodegenerative diseases, including SCI. Polypyrimidine tract binding protein 1 (PTBP1) has also associated with neural responses recovery. This study aims explore interaction between PTBP1 context of SCI, hypothesizing that regulates influence pathogenesis recovery using rat model lipopolysaccharide (LPS)-induced PC12 cells. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was performed measure levels, revealing its low rats. We then assessed neurological function, histopathological changes, spinal water content. found administering agomiR-10b-5p significantly improved function decreased content normal motor neuron loss Additionally, we explored functions LPS-treated Our results showed repressed LPS-stimulated apoptosis, inflammation, oxidative stress predicted as potential target TargetScan database. Pulldown luciferase reporter assays further demonstrated binds 3' untranslated region (UTR) PTBP1. RT-qPCR revealed negatively modulated both vivo vitro. Furthermore, rescue indicated alleviated rats LPS-triggered cells downregulating investigated on transforming growth factor-beta (TGF-β1)/small mother against decapentaplegic (Smad3) pathway. targeted repress TGF-β1 decay facilitated TGF-β1/Smad3 pathway activation. In conclusion, our demonstrate alleviates repressing inducing activation through downregulation. provides novel insights into therapy plans

Язык: Английский

Exploring Ubiquitination in Spinal Cord Injury Therapy: Multifaceted Targets and Promising Strategies DOI

Caizhen Shi,

Bingbing Wang,

Tianyu Zhai

и другие.

Neurochemical Research, Год журнала: 2025, Номер 50(1)

Опубликована: Янв. 20, 2025

Язык: Английский

Процитировано

0
MiR-10b-5p attenuates spinal cord injury and alleviates LPS-induced PC12 cells injury by inhibiting TGF-β1 decay and activating TGF-β1/Smad3 pathway through PTBP1 DOI Creative Commons

Huandong Liu,

Chong Liang,

Hongfei Liu

и другие.

European journal of medical research, Год журнала: 2024, Номер 29(1)

Опубликована: Ноя. 19, 2024

Spinal cord injury (SCI) is a debilitating condition characterized by significant sensory, motor, and autonomic dysfunctions, leading to severe physical, psychological, financial burdens. The current therapeutic approaches for SCI show limited effectiveness, highlighting the urgent need innovative treatments. MicroRNAs (miRNAs) like miR-10b-5p are known play pivotal roles in gene expression regulation have been implicated various neurodegenerative diseases, including SCI. Polypyrimidine tract binding protein 1 (PTBP1) has also associated with neural responses recovery. This study aims explore interaction between PTBP1 context of SCI, hypothesizing that regulates influence pathogenesis recovery using rat model lipopolysaccharide (LPS)-induced PC12 cells. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was performed measure levels, revealing its low rats. We then assessed neurological function, histopathological changes, spinal water content. found administering agomiR-10b-5p significantly improved function decreased content normal motor neuron loss Additionally, we explored functions LPS-treated Our results showed repressed LPS-stimulated apoptosis, inflammation, oxidative stress predicted as potential target TargetScan database. Pulldown luciferase reporter assays further demonstrated binds 3' untranslated region (UTR) PTBP1. RT-qPCR revealed negatively modulated both vivo vitro. Furthermore, rescue indicated alleviated rats LPS-triggered cells downregulating investigated on transforming growth factor-beta (TGF-β1)/small mother against decapentaplegic (Smad3) pathway. targeted repress TGF-β1 decay facilitated TGF-β1/Smad3 pathway activation. In conclusion, our demonstrate alleviates repressing inducing activation through downregulation. provides novel insights into therapy plans

Язык: Английский

Процитировано

0