Impairment of Endogenous H2S Pathway due to Aging and Endothelium Denudation in Mouse Isolated Thoracic Aorta
Physiological Research,
Год журнала:
2025,
Номер
1/2025, С. 59 - 68
Опубликована: Март 10, 2025
Hydrogen
sulfide
(H2S)
is
a
gas
neurotransmitter
that
synthesized
in
various
mammalian
tissues
including
vascular
and
regulates
tone.
The
aim
of
this
study
to
investigate
whether
the
endogenous
L-cysteine/H2S
pathway
impaired
due
aging
endothelial
denudation
mouse
isolated
thoracic
aorta.
For
purpose,
young
(3-4
months)
old
(23-25
mice
were
used
experiments.
effects
endothelium
on
exogenous
H2S-induced
vasorelaxation
investigated
by
cumulative
L-cysteine-(1
µM-10
mM)
NaHS-(1
µM-3
induced
vasorelaxations,
respectively.
L-cysteine-induced
relaxations
reduced
aorta
compared
mice.
Also,
vasorelaxant
responses
L-cysteine
(1
rings
with
denuded-endothelium
However,
relaxation
NaHS
not
altered
age
or
denudation.
loss
staining
CSE
layer
was
observed
Ach-induced
(1-30
µM)
almost
abolished
endothelium-denuded
from
both
group.
Ach
intact
tissue
In
conclusion,
but
decreased
protein
expression
consistent
decrease
H2S
concentration
damage,
suggesting
may
be
lead
enzyme
signaling
system
damage
Язык: Английский
Hydrogen sulfide preserves the function of senescent endothelium through SIRT2 mediated inflammatory inhibition
Journal of Molecular and Cellular Cardiology,
Год журнала:
2025,
Номер
unknown
Опубликована: Апрель 1, 2025
Язык: Английский
BMSC-derived exosome overexpressing GATA-4 suppresses H/R-induced cardiomyocyte ferroptosis through miR-330-3p/BAP1/SLC7A11/IP3R axis and mPTP opening
iScience,
Год журнала:
2024,
Номер
27(10), С. 110784 - 110784
Опубликована: Авг. 23, 2024
Язык: Английский
Design and Synthesis of 1,4‐Diformyl‐Piperazine Ferrostatin‐1 Derivatives as Novel Ferroptosis Inhibitors
Chemical Biology & Drug Design,
Год журнала:
2024,
Номер
104(5)
Опубликована: Окт. 28, 2024
ABSTRACT
The
present
study
focuses
on
the
design
and
synthesis
of
novel
1,4‐diformyl‐piperazine‐based
ferrostatin‐1
(Fer‐1)
derivatives,
their
evaluation
against
ferroptosis
activity.
synthesized
compounds
demonstrated
significant
anti‐ferroptosis
activity
in
human
umbilical
vascular
endothelial
cells
(HUVECs),
with
Compound
24
showing
highest
potency.
Mechanistic
studies
revealed
that
effectively
reduced
intracellular
reactive
oxygen
species
(ROS)
levels,
mitigated
mitochondrial
damage,
enhanced
glutathione
peroxidase
4
(GPX4)
expression.
Additionally,
exhibited
improved
solubility
plasma
stability
compared
to
control
compounds,
Fer‐1
JHL‐12.
These
findings
suggest
derivatives
hold
promise
as
therapeutic
agents
for
ferroptosis‐associated
cardiovascular
diseases.
Язык: Английский