Опубликована: Апрель 22, 2025

Abstract Expression of immediate early genes (IEGs) is critical for memory formation and has been widely used to identify the neural substrate traces, termed engram cells. However, types IEG have relatively not distinguished in identifying cells, as there limited knowledge about extent which different IEGs are selectively or concurrently involved engram. To address this question, we investigated combinative expression c-Fos, Arc, Npas4 proteins using immunohistochemistry following aversive rewarding experiences across subregions prefrontal cortex (PFC), basolateral amygdala (BLA), hippocampal dentate gyrus (DG), retrosplenial (RSC). Using an automated cell detection algorithm, found that patterns Npas4, Arc varied brain areas, with a higher increase expressing cells PFC posterior BLA than DG. The patterns, along their learning-induced changes, also differed areas; co-expression increased learning whereas was less pronounced DG RSC. Furthermore, demonstrate area-to-area functional connectivity networks were extracted by IEGs. These findings provide insights into how combinations will contribute deeper understanding significance IEG-tagged

Язык: Английский