Simplified MS2 phage-like particle production including a novel maturation protein internal fusion affinity tag

Biotechnology & Biotechnological Equipment, Год журнала: 2025, Номер 39(1)

Опубликована: Фев. 2, 2025

Phage-like particles (PLPs) are fabricated self-assembling nanoparticles derived from the structural elements of bacteriophages. These have biotechnological utility because ability to easily modify surface chemistry and compartmentalize nucleic acids or other materials. A consequential implementation PLPs in diagnostics is as process controls acid amplification tests, where control RNAs packaged within protein capsid protected degradation by RNases sample matrix. Key developments PLP enhanced packing efficiency into particles, reduced complexity their plasmid expression systems, shifted purification ultracentrifugation affinity chromatography, producing progressively greater yields with higher purity. Expanding on prior improvements, this study establishes a revised set vectors for Emesvirus zinderi (MS2) that streamline vector manipulations rapid prototyping new provide validation an alternative tag purification, contributes high-throughput low-volume spin column strategy. advancements combined novel internal fusion site MS2 maturation A, passive element designs, capable displaying polypeptides particles' surface. The functionality chimeric protein's display verified subsequent purification. This advancement increases number available peptide sites platform wide-ranging implications future applications.

Язык: Английский

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Recent progress and challenges in gene therapy for pharmacoresistant focal epilepsy

Revue Neurologique, Год журнала: 2025, Номер unknown

Опубликована: Март 1, 2025

Язык: Английский

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Evolving virus-like particles

Nature Biomedical Engineering, Год журнала: 2024, Номер unknown

Опубликована: Дек. 16, 2024

Язык: Английский

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