MYB transcription factors and their role in Medicinal plants

Molecular Biology Reports, Год журнала: 2022, Номер 49(11), С. 10995 - 11008

Опубликована: Сен. 8, 2022

Язык: Английский

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Genetic regulation of nitrogen use efficiency in Gossypium spp

Plant Cell & Environment, Год журнала: 2023, Номер 46(6), С. 1749 - 1773

Опубликована: Март 21, 2023

Cotton (Gossypium spp.) is the most important fibre crop, with desirable characteristics preferred for textile production. output relies heavily on nitrate as source of inorganic nitrogen (N). However, dynamics in extreme environments limit plant growth and lead to yield loss pollution. Therefore, use efficiency (NUE), which involves utilisation 'right rate', source', time', place' (4Rs), key efficient N management. Recent omics techniques have genetically improved NUE crops. We herein highlight mechanisms uptake assimilation vegetative reproductive branches cotton while considering known unknown regulatory factors. The phylogenetic relationships among transporters four Gossypium spp. been reviewed. Further, genes that participate xylem transport phloem loading are also discussed. In addition, functions microRNAs transcription factors modulating expression target highlighted. Overall, this review provides a detailed perspective complex mechanism cotton, would accelerate research toward improving

Язык: Английский

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Cooperative action of separate interaction domains promotes high-affinity DNA binding of Arabidopsis thaliana ARF transcription factors

Proceedings of the National Academy of Sciences, Год журнала: 2023, Номер 120(11)

Опубликована: Март 7, 2023

The signaling molecule auxin coordinates many growth and development processes in plants, mainly through modulating gene expression. Transcriptional response is mediated by the family of factors (ARF). Monomers this recognize a DNA motif can homodimerize their DNA-binding domain (DBD), enabling cooperative binding to an inverted site. Most ARFs further contain C-terminal PB1 that capable homotypic interactions mediating with Aux/IAA repressors. Given dual role domain, ability both DBD mediate dimerization, key question how these domains contribute specificity affinity. So far, ARF–ARF ARF–DNA have mostly been approached using qualitative methods do not provide quantitative dynamic view on equilibria. Here, we utilize assay based single-molecule Förster resonance energy transfer (smFRET) study affinity kinetics interaction several Arabidopsis thaliana IR7 auxin-responsive element (AuxRE). We show AtARF2 toward binding, identify ARF dimer stability as parameter defining across AtARFs. Lastly, derived analytical solution for four-state cyclic model explains between IR7. Our work demonstrates composite elements defined dimerization equilibrium, identifying ARF-mediated transcriptional activity.

Язык: Английский

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Transcriptional Control of Seed Life: New Insights into the Role of the NAC Family

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(10), С. 5369 - 5369

Опубликована: Май 14, 2024

Transcription factors (TFs) regulate gene expression by binding to specific sequences on DNA through their DNA-binding domain (DBD), a universal process. This update conveys information about the diverse roles of TFs, focusing NACs (NAM-ATAF-CUC), in regulating target-gene and influencing various aspects plant biology. NAC TFs appeared before emergence land plants. The family constitutes group plant-specific found mosses, conifers, monocots, eudicots. discusses evolutionary origins genes/proteins from green algae crucial development stress response across species. From mosses lycophytes angiosperms, number proteins increases significantly, suggesting gradual evolution basal streptophytic algae. play critical role enhancing abiotic tolerance, with function conserved angiosperms. Furthermore, modular organization NACs, dimeric function, localization within cellular compartments contribute functional versatility complexity. While most are nuclear-localized active, subset is other compartments, indicating inactive forms until cues trigger translocation nucleus. Additionally, it highlights involvement endoplasmic reticulum (ER) stress-induced programmed cell death (PCD) activating vacuolar processing enzyme (VPE) gene. Moreover, this provides comprehensive overview plants, including participation ER responses, leaf senescence (LS), growth development. Notably, exhibit correlations phytohormones (i.e., ABA, GAs, CK, IAA, JA, SA), several genes inducible them, broad spectrum biological processes. study spatiotemporal patterns insights into when where shedding light metabolic contributions. Likewise, review emphasizes significance transcriptional modules, seed reserve accumulation, regulation dormancy germination. Overall, effectively communicates intricate essential functions Finally, an standpoint, phylogenetic analysis suggests that highly probable WRKY evolutionarily older than family.

Язык: Английский

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Halotolerant bacterial endophyte Bacillus velezensis CBE mediates abiotic stress tolerance with minimal transcriptional modifications in Brachypodium distachyon

Frontiers in Plant Science, Год журнала: 2025, Номер 15

Опубликована: Янв. 10, 2025

Nitrogen and water are the primary resources limiting agricultural production worldwide. We have demonstrated ability of a novel halotolerant bacterial endophyte, Bacillus velezensis CBE, to induce osmotic stress tolerance in Brachypodium distachyon under nitrogen-deprived conditions. Additionally, we aimed identify molecular factors plants that contribute beneficial effects induced by B. CBE distachyon. To achieve this, conducted transcriptomic profiling using RNA-seq on 18-day-old seedlings treated with presence or absence available nitrogen, without stress. These profiles were then compared those obtained from known plant growth-promoting strains, Azospirillum brasilense Cd Azoarcus olearius DQS4, same growth identified differentially expressed genes (DEGs) response combinations strains treatments. Interestingly, only 73 transcripts showed significant differential expression CBE-treated conditions, 1,078 DEGs A. 2,015 DQS4. Our findings suggest endophyte mediates through fine-tuning mechanisms minimal transcriptional modifications.

Язык: Английский

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