Deep discovery informs difficult deployment in plant microbiome science

Cell, Год журнала: 2023, Номер 186(21), С. 4496 - 4513

Опубликована: Окт. 1, 2023

Plant-associated microbiota can extend plant immune system function, improve nutrient acquisition and availability, alleviate abiotic stresses. Thus, naturally beneficial microbial therapeutics are enticing tools to productivity. The basic definition of across species ecosystems, combined with the development reductionist experimental models manipulation phenotypes microbes, has fueled interest in its translation agriculture. However, great majority microbes exhibiting plant-productivity traits lab greenhouse fail field. Therapeutic must reach détente, establishment uneasy homeostasis, system, invade heterogeneous pre-established plant-associated communities, persist a new potentially remodeled community. Environmental conditions alter community structure thus impact engraftment therapeutic microbes. We survey recent breakthroughs, challenges, opportunities translating from

Язык: Английский

---

How plants manage pathogen infection

EMBO Reports, Год журнала: 2023, Номер 25(1), С. 31 - 44

Опубликована: Дек. 19, 2023

Abstract To combat microbial pathogens, plants have evolved specific immune responses that can be divided into three essential steps: recognition by receptors, signal transduction within plant cells, and execution directly suppressing pathogens. During the past decades, many receptors signaling components their mode of action been revealed, markedly advancing our understanding first two steps. Activation results in physical chemical actions actually stop pathogen infection. Nevertheless, this third step immunity is under explored. In addition to plants, recent evidence suggests microbiota, which considered an additional layer system, also plays a critical role direct suppression. review, we summarize current how as well microbiota control growth behavior highlight outstanding questions need answered.

Язык: Английский

---

Plant growth promoting rhizobacteria induced metal and salt stress tolerance in Brassica juncea through ion homeostasis

Ecotoxicology and Environmental Safety, Год журнала: 2023, Номер 267, С. 115657 - 115657

Опубликована: Ноя. 1, 2023

Soil heavy metal contamination and salinity constitute a major environmental problem worldwide. The affected area impact of these problems are increasing day by day; therefore, it is imperative to restore their potential using environmentally friendly technology. Plant growth-promoting rhizobacteria (PGPR) provides better option in this context. Thirty-seven bacteria were isolated from the rhizosphere maize cultivated metal- salt-affected soils. Some selected bacterial strains grew well under wide range pH (4−10), salt (5–50 g/L), Cd (50–1000 mg/L) stress. Three strains, Exiguobacterium aestuarii (UM1), Bacillus cereus (UM8), megaterium (UM35), because robust growth high tolerance both stress conditions. UM1, UM8, UM35 showed P-solubilization, whereas UM8 exhibited 1-aminocyclopropane-1-carboxylate deaminase activity indole acetic acid (IAA) production, respectively. inoculated on Brassica juncea plants soils due above PGP activities tolerance. Plants with B. significantly (p < 0.05) increased shoot fresh weight (17 ± 1.17–29 0.88 g/plant), dry (2.50 0.03–4.40 0.32 root (7.30 0.58–13.30 0.58 (0.80 0.04–2.00 0.01 K contents (62.76 1.80–105.40 1.15 mg/kg dwt) normal stressful strain decreased Na+ Cd++ uptake single dual Both E. cereus, efficiently reduced translocation bioaccumulation shoot. Bacterial inoculation improved K+ Ca++, while restricted shoots indicated mitigate stresses through ion homeostasis.

Язык: Английский

---

Importance of pathobiomes to the success of microbial weed biocontrol agents

Biological Control, Год журнала: 2024, Номер 192, С. 105498 - 105498

Опубликована: Март 15, 2024

The role of microbial communities in the establishment plant pathogens introduced to new environments as weed biocontrol agents is relatively unexplored. Substantial evidence from crop protection research shows how bulk influence establishment, survival, and success pathogens. Just mismatches between agents, host genotypes, climate can lead failure, we propose that resident microbiomes may be a cause apparent failures. From this perspective, discuss recent progress on plant-associated whose interactions disease development, or "pathobiomes," consider implications for agent evaluation release. Open questions about pathobiomes develop rates provide ample opportunity researchers adapt methods analytical frameworks will enhance programs. We conclude by prioritizing development standard include component release studies. Developing pathobiome-aware selection process improve outcomes.

Язык: Английский

---

Plant secondary metabolites-mediated plant defense against bacteria and fungi pathogens

Plant Physiology and Biochemistry, Год журнала: 2024, Номер 217, С. 109224 - 109224

Опубликована: Окт. 20, 2024

Язык: Английский

---

Streamlining plant disease diagnosis with convolutional neural networks and edge devices

Neural Computing and Applications, Год журнала: 2024, Номер 36(29), С. 18445 - 18477

Опубликована: Июль 29, 2024

Язык: Английский

---

Microbiome-mediated plant disease resistance: recent advances and future directions

Journal of General Plant Pathology, Год журнала: 2024, Номер unknown

Опубликована: Окт. 17, 2024

Abstract Plant pathogens cause plant diseases that significantly decrease crop yields, posing a serious threat to global food security. While disease resistance has traditionally been understood as the trait determined by innate immune system and pathogen virulence system, recent research underscores pivotal role of microbiome in resistance. Plant-associated microbiomes confer protection against through direct inhibition, resource competition, activation responses. Agricultural practices such rotation, intercropping, disease-resistant breeding, biocontrol, organic farming modulate microbiomes, thereby influencing This review synthesizes latest advancements understanding intricate interactions among plants, pathogens, microbiomes. We emphasize need for in-depth mechanistic studies linking agricultural dynamics propose future directions leverage sustainable agriculture.

Язык: Английский

---

Enhanced multiscale plant disease detection with the PYOLO model innovations

Scientific Reports, Год журнала: 2025, Номер 15(1)

Опубликована: Фев. 12, 2025

Abstract Timely detection of plant diseases is crucial for agricultural safety, product quality, and environmental protection. However, disease faces several challenges, including the diversity scenarios complex backgrounds. To address these issues, we propose a model named PYOLO. Firstly, enhances feature fusion capabilities by optimizing PAN structure, introducing weighted bidirectional pyramid network (BiFPN), repeatedly fusing top bottom scale features. Additionally, model’s ability to focus on different parts image improved redesigning EC2f structure dynamically adjusting convolutional kernel size better capture features at various scales. Finally, MHC2f mechanism designed enhance perceive backgrounds targets scales utilizing its self-attention parallel processing. Experiments demonstrate that mAP value increases 4.1% compared YOLOv8n, confirming superiority in detection.

Язык: Английский

---

Salt stress alters the selectivity of mature pecan for the rhizosphere community and its associated functional traits

Frontiers in Plant Science, Год журнала: 2025, Номер 16

Опубликована: Март 26, 2025

Introduction Salt stress is a major global environmental factor limiting plant growth. Rhizosphere bacteria, recruited from bulk soil, play pivotal role in enhancing salt resistance herbaceous and crop species. However, whether the rhizosphere bacterial community of mature tree can respond to stress, particularly saline-alkalitolerant trees, remains unexplored. Pecan ( Carya illinoinensis ), an important commercially cultivated nut tree, considered saline-alkali tolerant. Methods trees (12 years) were subjected different NaCl concentrations for 12 weeks. Collected samples included roots, leaves, fruit. Amplicon sequencing data shotgun metagenomic obtained investigated: 1) microbial communities various ecological niches pecan trees; 2) characteristic bacteria associated functional traits when suffered stress. Results discussion We characterized pecan-associated microbiome (i.e., fruit, leaf, root, soil) first time. These findings suggest that niche-based processes, such as habitat selection, drive fungal assembly tissues. reduced diversity, altered composition, shifted pecan’s selective pressure on Proteobacteria Actinobacteria . Shotgun further revealed response This study enhances our understanding tree-associated microbiomes supports theory shaping may be strategy saline-alkali-tolerant resist provide insights into tolerance potential applications, development bio-inoculants, managing saline environments agricultural contexts.

Язык: Английский

---

Time-Course Responses of Apple Leaf Endophytes to the Infection of Gymnosporangium yamadae

Journal of Fungi, Год журнала: 2024, Номер 10(2), С. 128 - 128

Опубликована: Фев. 3, 2024

Apple rust, caused by Gymnosporangium yamadae, poses a significant challenge to apple production. Prior studies have underscored the pivotal role played endophytic microbial communities, intimately linked with host, in influencing plant diseases and their pathogenic outcomes. The objective of this study is scrutinize alternations communities within leaves at different stages rust using high-throughput sequencing technology. findings revealed discernible pattern characterized an initial increase subsequent decrease alpha diversity diseased leaves. A co-occurrence network analysis that complexity bacterial community diminished initially then rebounded during progression disease. Additionally, employing PICRUSt2 platform, provided preliminary insights into functions specific disease timepoints. During spermogonial stage, bacteria particularly exhibited heightened activity genetic information processing, metabolism, environmental processing pathways. Endophytic fungi also significantly enriched large number metabolic pathways stage aecial exhibiting abnormally active life activities. These establish foundation for comprehending host endophytes interaction between pathogens hosts. Furthermore, they offer valuable development exploitation resources, thereby contributing enhanced strategies managing rust.

Язык: Английский

---