Origin and Evolution of IQD Scaffolds and Assembled Protein Complexes in Plant Cell Division DOI
Pradeep Dahiya, Samuel Haluška, Jonas Buhl

и другие.

Опубликована: Янв. 1, 2023

In ancestors of modern-day streptophyte algae, cell division has undergone a switch from cleavage-like mode to an inside-out mechanism, in which new walls are inserted at the center and expand centrifugally, eventually fusing with maternal wall specific cortical region, termed zone (CDZ) 1-3. This involved stepwise evolution two novel cytoskeleton arrays, phragmoplast preprophase band (PPB). The PPB/phragmoplast system possibly provided basis for tunable orientation, enabled 3D development morphological adaptations required successful colonization terrestrial habitats4. How acquired its functions, however, is still largely enigmatic. Our previous work identified IQ67-DOMAIN8 (IQD8) Arabidopsis thaliana as important determinant PPB formation plane positioning5,6. IQD8 intrinsically disordered scaffold protein that interacts core components CDZ7. Here, through phylogenetic functional analyses, we show IQDs emerged last common ancestor Klebsormidiophyceae Phragmoplastophyta algae. Gradual changes motif composition acquisition likely facilitated diversification terms subcellular localization protein-protein interactions. Cross-complementation studies mutants provide evidence evolutionarily conserved functions land-plant key regulators control. summary, our establishes plant-specific proteins, played role rewiring neofunctionalization interaction networks distinct sites facilitate evolutionary apparatus microtubule general.

Язык: Английский

The ER–PM interaction is essential for cytokinesis and recruits the actin cytoskeleton through the SCAR/WAVE complex DOI Creative Commons
Zhijing Xu, Jingze Zang, Xintong Zhang

и другие.

Proceedings of the National Academy of Sciences, Год журнала: 2025, Номер 122(6)

Опубликована: Фев. 6, 2025

Plant cytokinesis requires coordination between the actin cytoskeleton, microtubules, and membranes to guide division plane formation cell plate expansion; how these regulatory factors are coordinated remains unknown. The cytoskeleton assembly is controlled by several nucleation factors, such as SCAR/WAVE complex, which regulates branching through activation of ARP2/3 complex. activity proteins likely influenced interactions with specific membranes; however, molecular basis biological relevance SCAR–membrane also unclear. In this study, we demonstrate that ER–PM tethering protein VAP27-1 directly interacts SCAR2 at ER membrane they colocalize orientation during division. root meristem, both exhibit polarized localization plates, where interaction PM abundant. recruits plane, there a high concentration filaments. vap27-1346 mutant, densities cortical ER, SCAR2, consequently filaments significantly reduced affecting orientation, division, development. A similar phenomenon observed in scar1234 suggesting VAP27 SCAR regulate pathway. conclusion, our data reveal plant-specific function VAP27-regulated advance understanding plant contact site its role

Язык: Английский

Процитировано

0
Dehydration-responsive cytoskeleton proteome of rice reveals reprograming of key molecular pathways to mediate metabolic adaptation and cell survival DOI
Sunil Kumar, Subhra Chakraborty, Niranjan Chakraborty

и другие.

Plant Physiology and Biochemistry, Год журнала: 2024, Номер 207, С. 108359 - 108359

Опубликована: Янв. 18, 2024

Язык: Английский

Процитировано

1
Spatiotemporal regulation of plant cell division DOI Creative Commons
David Bouchez, Magalie Uyttewaal, Martine Pastuglia

и другие.

Current Opinion in Plant Biology, Год журнала: 2024, Номер 79, С. 102530 - 102530

Опубликована: Апрель 16, 2024

Plant morphogenesis largely depends on the orientation and rate of cell division elongation, their coordination at all levels organization. Despite recent progresses in comprehension pathways controlling plane determination plant cells, many pieces are missing to puzzle. For example, we have a partial formation, function evolutionary significance preprophase band, plant-specific cytoskeletal array involved premitotic setup plane, as well role nucleus its connection band microtubules. Likewise, several modeling studies point strong relationship between shape geometry, but emergence such geometric rules from molecular cellular play still obscure. Yet, imaging technologies genetic tools hold lot promise tackle these challenges revisit old questions with unprecedented resolution space time.

Язык: Английский

Процитировано

1
Origin and Evolution of IQD Scaffolds and Assembled Protein Complexes in Plant Cell Division DOI
Pradeep Dahiya, Samuel Haluška, Jonas Buhl

и другие.

Опубликована: Янв. 1, 2023

In ancestors of modern-day streptophyte algae, cell division has undergone a switch from cleavage-like mode to an inside-out mechanism, in which new walls are inserted at the center and expand centrifugally, eventually fusing with maternal wall specific cortical region, termed zone (CDZ) 1-3. This involved stepwise evolution two novel cytoskeleton arrays, phragmoplast preprophase band (PPB). The PPB/phragmoplast system possibly provided basis for tunable orientation, enabled 3D development morphological adaptations required successful colonization terrestrial habitats4. How acquired its functions, however, is still largely enigmatic. Our previous work identified IQ67-DOMAIN8 (IQD8) Arabidopsis thaliana as important determinant PPB formation plane positioning5,6. IQD8 intrinsically disordered scaffold protein that interacts core components CDZ7. Here, through phylogenetic functional analyses, we show IQDs emerged last common ancestor Klebsormidiophyceae Phragmoplastophyta algae. Gradual changes motif composition acquisition likely facilitated diversification terms subcellular localization protein-protein interactions. Cross-complementation studies mutants provide evidence evolutionarily conserved functions land-plant key regulators control. summary, our establishes plant-specific proteins, played role rewiring neofunctionalization interaction networks distinct sites facilitate evolutionary apparatus microtubule general.

Язык: Английский

Процитировано

0