Composition of saliva metabolome is significantly associated with SARS-CoV2 infection and with severity of COVID-19 disease DOI Creative Commons

Violeta Larios-Serrato,

Natalia Vázquez-Manjarrez, Osbaldo Resendis‐Antonio

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Окт. 25, 2024

Abstract Background The metabolome of COVID-19 patients has been studied sparsely, with most research focusing on a limited number plasma metabolites or small cohorts. This is the first study to test saliva in comprehensive way, revealing significant changes linked disease severity and highlighting potential as non-invasive diagnostic tool. Methods We included 30 asymptomatic subjects no prior infection vaccination, 102 mild SARS-CoV-2 infection, 61 hospitalized confirmed status. Saliva samples were analyzed using hydrophilic interaction liquid chromatography-mass spectrometry (HILIC-MS/MS) positive negative ionization modes. Results Significant identified patients, distinct patterns based severity. Healthy individuals exhibited well-regulated bacterial network, while severe cases showed disordered microbial networks. Elevated dipeptides such Val-Glu Met-Gln moderate suggest specific protease activity related SARS-CoV-2. Increased acetylated amino acids like N-Acetylserine N-Acetylhistidine indicate biomarkers for stress Bacterial metabolites, including muramic acid indole-3-carboxaldehyde, higher mild-moderate cases, indicating oral microbiota changes. In polyamines organ damage-related N-acetylspermine 3-methylcytidine, significantly increased. Interestingly, reduced elevated cases. Conclusions metabolomics offers insight into progression COVID-19.

Язык: Английский

Protein hydrolysates enhance recovery from drought stress in tomato plants: phenomic and metabolomic insights DOI Creative Commons

Marzia Leporino,

Youssef Rouphael, Paolo Bonini

и другие.

Frontiers in Plant Science, Год журнала: 2024, Номер 15

Опубликована: Март 12, 2024

Introduction High-throughput phenotyping technologies together with metabolomics analysis can speed up the development of highly efficient and effective biostimulants for enhancing crop tolerance to drought stress. The aim this study was examine morphophysiological metabolic changes in tomato plants foliarly treated two protein hydrolysates obtained by enzymatic hydrolysis vegetal proteins from Malvaceae (PH1) or Fabaceae (PH2) comparison a control treatment, as well investigate mechanisms involved enhancement plant resistance repeated stress cycles. Methods A device used daily monitoring traits while untargeted carried out leaves best performing treatment based on phenotypic results. Results PH1 most water due better recovery digital biomass 3D leaf area after each event PH2 mitigating only during period first event. Metabolomics data indicated that modified primary metabolism increasing concentration dipeptides fatty acids untreated control, secondary regulating several compounds like phenols. In contrast, hormones detoxification signal molecules against reactive oxygen species were downregulated control. Conclusion above findings demonstrated advantages combined phenomics-metabolomics approach elucidating relationship between associated biostimulant-mediated increase events.

Язык: Английский

Процитировано

7
Metabolic changes in tomato plants caused by psychrotolerant Antarctic endophytic bacteria might be implicated in cold stress mitigation DOI Creative Commons

Giorgio Licciardello,

Maria Doppler,

Carmela Sicher

и другие.

Physiologia Plantarum, Год журнала: 2024, Номер 176(3)

Опубликована: Май 1, 2024

Abstract Climate change is responsible for mild winters and warm springs that can induce premature plant development, increasing the risk of exposure to cold stress with a severe reduction in growth. Tomato plants are sensitive beneficial microorganisms increase their tolerance. However, scarce information available on mechanisms stimulated by bacterial endophytes tomato against stress. This study aimed clarify metabolic changes psychrotolerant endophytic bacteria exposed annotate compounds possibly associated mitigation. seeds were inoculated two isolated from Antarctic Colobanthus quitensis ( Ewingella sp. S1.OA.A_B6 Pseudomonas S2.OTC.A_B10) or Paraburkholderia phytofirmans PsJN, while mock‐inoculated used as control. The composition was analyzed immediately after (4°C seven days) four days recovery at 25°C. Under stress, content malondialdehyde, phenylalanine, ferulic acid, p‐coumaric acid lower bacterium‐inoculated compared plants, indicating lipid peroxidation stimulation phenolic compound metabolism. compounds, five putative phenylalanine‐derived dipeptides, three further higher samples under Thus, reprogram polyphenol metabolism stimulate accumulation secondary metabolites, like 4‐hydroxybenzoic salicylic which presumably involved mitigation, dipeptides responses.

Язык: Английский

Процитировано

4
Current insights into molecular mechanisms of environmental stress tolerance in Cyanobacteria DOI

Preeti Rai,

Ruchi Pathania, N. T. Bhagat

и другие.

World Journal of Microbiology and Biotechnology, Год журнала: 2025, Номер 41(2)

Опубликована: Янв. 28, 2025

Язык: Английский

Процитировано

0
Effects of Lacticaseibacillus casei Zhang addition on physicochemical properties and metabolomics of fermented camel milk during storage DOI Creative Commons

Dandan Wang,

Wusigale,

Lu Li

и другие.

Food Chemistry X, Год журнала: 2025, Номер 26, С. 102318 - 102318

Опубликована: Фев. 1, 2025

Язык: Английский

Процитировано

0
Transport of tryptophan dipeptide derivatives and impact on inflammatory cytokines in monocyte/macrophage-like cells. DOI Creative Commons
Jae B. Park, Renee Peters

Heliyon, Год журнала: 2025, Номер 11(5), С. e43000 - e43000

Опубликована: Фев. 27, 2025

Язык: Английский

Процитировано

0
Analyses of Saliva Metabolome Reveal Patterns of Metabolites That Differentiate SARS-CoV-2 Infection and COVID-19 Disease Severity DOI Creative Commons
Violeta Larios‐Serrato, Natalia Vázquez-Manjarrez, Osbaldo Resendis‐Antonio

и другие.

Metabolites, Год журнала: 2025, Номер 15(3), С. 192 - 192

Опубликована: Март 11, 2025

Background: The metabolome of COVID-19 patients has been studied sparsely, with most research focusing on a limited number plasma metabolites or small cohorts. This is the first study to test saliva in comprehensive way, revealing patterns significantly linked disease and severity, highlighting saliva’s potential as non-invasive tool for pathogenesis diagnostic studies. Methods: We included 30 asymptomatic subjects no prior infection vaccination, 102 mild SARS-CoV-2 infection, 61 hospitalized confirmed status. Saliva samples were analyzed using hydrophilic interaction liquid chromatography–mass spectrometry (HILIC-MS/MS) positive negative ionization modes. Results: Significant differences identified patients, distinct associated severity. Dipeptides such Val-Glu Met-Gln highly elevated moderate cases, suggesting specific protease activity related SARS-CoV-2. Acetylated amino acids like N-acetylserine N-acetylhistidine increased severe cases. Bacterial metabolites, including muramic acid indole-3-carboxaldehyde, higher mild–moderate indicating that oral microbiota differs according In polyamines organ-damage-related N-acetylspermine 3-methylcytidine, increased. Interestingly, reduced cases Conclusions: metabolomics offers insightful information potentially useful studying severity diagnosis.

Язык: Английский

Процитировано

0
Impact of a Synthetic Microbial Community on Salvia officinalis Under Optimal and Reduced Irrigation DOI Creative Commons

Renée Abou Jaoudé,

Anna Grazia Ficca, Francesca Luziatelli

и другие.

Horticulturae, Год журнала: 2025, Номер 11(4), С. 374 - 374

Опубликована: Март 31, 2025

Water scarcity can negatively affect crop yield, posing a significant threat to global food security, such as drought. Plant growth-promoting rhizobacteria (PGPR), either single strains or synthetic communities (SynComs), has shown promise in alleviating drought stress various plant species. In this study, we examined the effects of water limitation on Salvia officinalis and potential SynCom composed five phosphate-solubilizing, auxin-producing, and/or nitrogen-fixing Gram-negative bacteria enhance growth tolerance. growth, morphology, physiology, leaf metabolomic profiles were assessed using combination physiological measurements LC-MS untargeted metabolomics. Mild induced conservative water-use strategy S. officinalis, characterized by increased root-to-shoot ratio altered without compromising photosynthetic performance. inoculation under well-watered conditions elicited drought-like responses, including transient reductions stomatal conductance. Leaf analysis revealed that influenced abundance several metabolites, biogenic amines dipeptides, both irrigation regimes. Notably, histamine α-ketoglutaric acid levels, highlighting impacts quality. Under reduced irrigation, further modulated morphology biomass allocation, promoting thicker leaves root allocation. These results demonstrate ability modulate physiology metabolism response optimal potentially enhancing resilience directly improving growth. The study also highlights complex interactions among microbial inoculation, metabolite profiles, emphasizing importance considering production bioactive compounds when developing inoculants for edible plants.

Язык: Английский

Процитировано

0
Root microbiota regulates tiller number in rice DOI Creative Commons
Jingying Zhang, Bing Wang, Haoran Xu

и другие.

Cell, Год журнала: 2025, Номер unknown

Опубликована: Апрель 1, 2025

Язык: Английский

Процитировано

0
Polychlorinated biphenyls modify Arabidopsis root exudation pattern to accommodate degrading bacteria, showing strain and functional trait specificity DOI Creative Commons
Eleonora Rolli, Elisa Ghitti, Francesca Mapelli

и другие.

Frontiers in Plant Science, Год журнала: 2024, Номер 15

Опубликована: Июль 5, 2024

Introduction The importance of plant rhizodeposition to sustain microbial growth and induce xenobiotic degradation in polluted environments is increasingly recognized. Methods Here the “cry-for-help” hypothesis, consisting root chemistry remodeling upon stress, was investigated presence polychlorinated biphenyls (PCBs), highly recalcitrant phytotoxic compounds, highlighting its role reshaping nutritional signaling features niche accommodate PCB-degrading microorganisms. Results Arabidopsis exposure 70 µM PCB-18 triggered plant-detrimental effects, stress-related traits, PCB-responsive gene expression, reproducing PCB phytotoxicity. exudates plantlets exposed for 2 days pollutant were collected characterized through untargeted metabolomics analysis by liquid chromatography–mass spectrometry. Principal component disclosed a different exudation fingerprint PCB-18-exposed plants, potentially contributing event. To investigate this aspect, five compounds identified exudate metabolomic (i.e., scopoletin, N-hydroxyethyl-β-alanine, hypoxanthine, L-arginyl-L-valine, L-seryl-L-phenylalanine) assayed their influence on physiology functionality strains Pseudomonas alcaliphila JAB1, Paraburkholderia xenovorans LB400, Acinetobacter calcoaceticus P320. Scopoletin, whose relative abundance decreased PCB-18-stressed exudates, hampered proliferation JAB1 P320, presumably due antimicrobial activity, reduced beneficial effect which showed higher degree promotion scopoletin-depleted mutant f6’h1 compared WT plants PCB. Nevertheless, scopoletin induced expression bph catabolic operon LB400. primary metabolites L-seryl-L-phenylalanine, increased preferentially used as nutrients growth-stimulating factors three degrading variable ability affect rhizocompetence traits like motility biofilm formation. Discussion These findings expand knowledge PCB-triggered steering microbiome boost holobiont fitness environments.

Язык: Английский

Процитировано

2
Proteome and metabolomic profile of Mongolian horse follicular fluid during follicle development DOI Creative Commons
Xinyu Li,

Ming Du,

Yuanyi Liu

и другие.

Scientific Reports, Год журнала: 2024, Номер 14(1)

Опубликована: Авг. 26, 2024

During follicular development, changes in the composition of fluid are synchronized with development oocytes. Our aim was to screen key factors affecting oocyte maturation and optimize vitro culture protocol by understanding proteins metabolites fluid. Follicles divided into three groups according their diameter (small follicle (SFF): 10 mm < d 20 mm; medium (MFF): 30 large (LFF): d). Proteins from were analyzed mass spectrometry. The results showed that: LFF vs MFF, differential abundant protein (DAP) 88 (DAM) screened out; In SFF 3 DAPs 65 DAMs MFF SFF, 24 35 out. analysis that glycerophosphate hydrolysis decreased during played a major role metabolism binding. addition, co-enriched "linoleic acid metabolism" pathway. Combinatorial reveals dynamic profile provides fundation for further exploring function Mongolian horse.

Язык: Английский

Процитировано

2