The next Green Revolution: integrating crop architectype and physiotype

Trends in biotechnology, Год журнала: 2025, Номер unknown

Опубликована: Апрель 1, 2025

Язык: Английский

Insertional Mutagenesis as a Strategy to Open New Paths in Microalgal Molybdenum and Nitrate Homeostasis

Current Issues in Molecular Biology, Год журнала: 2025, Номер 47(6), С. 396 - 396

Опубликована: Май 26, 2025

Molybdenum (Mo) is a vital micronutrient for nearly all living organisms, serving as cofactor molybdoenzymes that catalyze essential redox reactions in nitrogen metabolism. Among these enzymes, nitrate reductase plays crucial role assimilation. Maintaining Mo homeostasis—including uptake, storage, and utilization—is critical to avoid both deficiency toxicity. Our research focuses on uncovering novel molecular components involved homeostasis, particularly connection with assimilation, using Chlamydomonas reinhardtii, model green microalga. To achieve this, we generated more than 5000 transformants through insertional mutagenesis paromomycin resistance cassette (AphVIII) screened them altered growth under different concentrations. We identified four strains showing patterns when source or exhibiting increased sensitivity Mo. The genomic alterations were identified. Notably, Mo-resistant Mo-sensitive transformant had disruptions the genes encoded ABC-type transport proteins, indicating potential proteins transport. Additionally, two unable grow nitrate. One of mutation CNX7, gene biosynthesis, while other BAT1, an amino acid transporter. BAT1 mutant represents interesting case study, this has not previously been associated These findings enhance our understanding homeostasis mechanisms open new paths engineering microalgae improved

Язык: Английский

Towards establishing functional nitrogenase activities within plants

Trends in biotechnology, Год журнала: 2025, Номер unknown

Опубликована: Май 1, 2025

Язык: Английский