Reduction of Multispecies Biofilms on an Acrylic Denture Base Model by Antimicrobial Photodynamic Therapy Mediated by Natural Photosensitizers DOI Creative Commons

Ali Shahi Ardakani,

Stefano Benedicenti, Luca Solimei

и другие.

Pharmaceuticals, Год журнала: 2024, Номер 17(9), С. 1232 - 1232

Опубликована: Сен. 18, 2024

Objectives: The aim of this study is to investigate the antimicrobial efficacy photodynamic therapy (PDT) using natural photosensitizers (curcumin, riboflavin, and phycocyanin) light-emitting diode (LED) irradiation against multispecies biofilms in an acrylic denture base model. Materials Methods: Forty-five specimens were fabricated heat-curing resin. then infected with a mixed culture bacterial fungal species (including Streptococcus mutans, sanguinis, Candida albicans, glabrata) for 4 days. discs divided into nine groups, each group containing five discs: control, 0.2% chlorhexidine, 5.25% sodium hypochlorite, curcumin, phycocyanin alone or along LED. After treatment, number colony-forming units (CFUs) per milliliter was counted. In addition, extent biofilm degradation assessed crystal violet staining method scanning electron microscopy. Results: All experimental groups exhibited significant reduction colony numbers both compared control (p < 0.001). PDT statistically counts bacteria fungi photosensitizer-only groups. Conclusions: results vitro show that LED devices can effectively reduce viability eradicate microorganisms responsible causing infections.

Язык: Английский

Reduction of Multispecies Biofilms on an Acrylic Denture Base Model by Antimicrobial Photodynamic Therapy Mediated by Natural Photosensitizers DOI Creative Commons

Ali Shahi Ardakani,

Stefano Benedicenti, Luca Solimei

и другие.

Pharmaceuticals, Год журнала: 2024, Номер 17(9), С. 1232 - 1232

Опубликована: Сен. 18, 2024

Objectives: The aim of this study is to investigate the antimicrobial efficacy photodynamic therapy (PDT) using natural photosensitizers (curcumin, riboflavin, and phycocyanin) light-emitting diode (LED) irradiation against multispecies biofilms in an acrylic denture base model. Materials Methods: Forty-five specimens were fabricated heat-curing resin. then infected with a mixed culture bacterial fungal species (including Streptococcus mutans, sanguinis, Candida albicans, glabrata) for 4 days. discs divided into nine groups, each group containing five discs: control, 0.2% chlorhexidine, 5.25% sodium hypochlorite, curcumin, phycocyanin alone or along LED. After treatment, number colony-forming units (CFUs) per milliliter was counted. In addition, extent biofilm degradation assessed crystal violet staining method scanning electron microscopy. Results: All experimental groups exhibited significant reduction colony numbers both compared control (p < 0.001). PDT statistically counts bacteria fungi photosensitizer-only groups. Conclusions: results vitro show that LED devices can effectively reduce viability eradicate microorganisms responsible causing infections.

Язык: Английский

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