ACS Applied Materials & Interfaces,
Год журнала:
2024,
Номер
16(26), С. 33070 - 33080
Опубликована: Июнь 21, 2024
Nanomaterials
have
been
extensively
exploited
in
tumor
treatment,
leading
to
numerous
innovative
strategies
for
cancer
therapy.
While
nanomedicines
present
immense
potential,
their
application
therapy
is
characterized
by
significant
complexity
and
unpredictability,
especially
regarding
biocompatibility
anticancer
efficiency.
These
considerations
underscore
the
essential
need
development
of
ex
vivo
research
models,
which
provide
invaluable
insights
understanding
into
biosafety
efficacy
oncology.
Fortunately,
emergence
organoid
technology
offers
a
novel
approach
preclinical
evaluation
vitro.
Hence,
this
study,
we
constructed
intestine
hepatocyte
models
(Intestine-orgs
Hep-orgs)
assessing
intestinal
hepatic
toxicity
at
microtissue
level.
We
utilized
three
typical
metal–organic
frameworks
(MOFs),
ZIF-8,
ZIF-67,
MIL-125,
as
further
detect
interactions
with
organoids.
Subsequently,
MIL-125
loaded
methotrexate
(MTX),
forming
nanomedicine
(MIL-125-PEG-MTX),
indicated
high
loading
efficiency
(82%)
well-release
capability
an
acid
microenvironment.
More
importantly,
effect
was
investigated
using
vitro
patient-derived
organoids
(PDOs)
model,
achieving
inhibition
rates
48%
78%
PDO-1
PDO-2,
respectively,
demonstrating
that
PDOs
could
predict
clinical
response
facilitate
prospective
therapeutic
selection.
achievements
presented
great
potential
organoid-based
nano
theragnostic
function.
Molecular Biomedicine,
Год журнала:
2024,
Номер
5(1)
Опубликована: Фев. 12, 2024
Abstract
Cancer
is
associated
with
a
high
degree
of
heterogeneity,
encompassing
both
inter-
and
intra-tumor
along
considerable
variability
in
clinical
response
to
common
treatments
across
patients.
Conventional
models
for
tumor
research,
such
as
vitro
cell
cultures
vivo
animal
models,
demonstrate
significant
limitations
that
fall
short
satisfying
the
research
requisites.
Patient-derived
organoids,
which
recapitulate
structures,
specific
functions,
molecular
characteristics,
genomics
alterations
expression
profiles
primary
tumors.
They
have
been
efficaciously
implemented
illness
portrayal,
mechanism
exploration,
high-throughput
drug
screening
assessment,
discovery
innovative
therapeutic
targets
potential
compounds,
customized
treatment
regimen
cancer
In
contrast
conventional
organoids
offer
an
intuitive,
dependable,
efficient
model
by
conserving
phenotypic,
genetic
diversity,
mutational
attributes
originating
tumor.
Nevertheless,
organoid
technology
also
confronts
bottlenecks
challenges,
how
comprehensively
reflect
microenvironment,
angiogenesis,
reduce
costs,
establish
standardized
construction
processes
while
retaining
reliability.
This
review
extensively
examines
use
techniques
fundamental
precision
medicine.
It
emphasizes
importance
patient-derived
biobanks
development,
screening,
safety
evaluation,
personalized
Additionally,
it
evaluates
application
experimental
better
understand
mechanisms
The
intent
this
explicate
significance
present
new
avenues
future
research.
Ecotoxicology and Environmental Safety,
Год журнала:
2022,
Номер
235, С. 113429 - 113429
Опубликована: Март 21, 2022
Tremendous
progress
has
been
made
in
the
field
of
toxicology
leading
to
advance
developmental
toxicity
assessment.
Conventional
animal
models
and
vitro
two-dimensional
cannot
accurately
describe
toxic
effects
predict
actual
vivo
responses
due
obvious
inter-species
differences
between
humans
animals,
as
well
lack
a
physiologically
relevant
tissue
microenvironment.
Human
embryonic
stem
cell
(hESC)-
induced
pluripotent
(iPSC)-derived
three-dimensional
organoids
are
ideal
complex
multicellular
organotypic
models,
which
indispensable
recapitulating
morphogenesis,
cellular
interactions,
molecular
processes
early
human
organ
development.
Recently,
have
used
for
drug
discovery,
chemical
safety
This
review
discusses
recent
advances
use
organoid
(i.e.,
brain,
retinal,
cardiac,
liver,
kidney,
lung,
intestinal
models)
teratogenicity
assessment
distinct
tissues/organs
following
exposure
pharmaceutical
compounds,
heavy
metals,
persistent
organic
pollutants,
nanomaterials,
ambient
air
pollutants.
Combining
next-generation
with
innovative
engineering
technologies
generates
novel
powerful
tools
assessment,
rapid
this
is
expected
continue.
Theranostics,
Год журнала:
2024,
Номер
14(8), С. 3300 - 3316
Опубликована: Янв. 1, 2024
Patient-derived
organoids
(PDOs)
have
emerged
as
a
promising
platform
for
clinical
and
translational
studies.
A
strong
correlation
exists
between
outcomes
the
use
of
PDOs
to
predict
efficacy
chemotherapy
and/or
radiotherapy.
To
standardize
interpretation
enhance
scientific
communication
in
field
cancer
precision
medicine,
we
revisit
concept
PDO-based
drug
sensitivity
testing
(DST).
We
present
an
expert
consensus-driven
approach
medication
selection
aimed
at
predicting
patient
responses.
further
DST,
propose
guidelines
clarification
characterization.
Additionally,
identify
several
major
challenges
prediction
when
utilizing
PDOs.
Theranostics,
Год журнала:
2024,
Номер
14(7), С. 2946 - 2968
Опубликована: Янв. 1, 2024
Recent
advancements
in
modern
science
have
provided
robust
tools
for
drug
discovery.
The
rapid
development
of
transcriptome
sequencing
technologies
has
given
rise
to
single-cell
transcriptomics
and
single-nucleus
transcriptomics,
increasing
the
accuracy
accelerating
discovery
process.
With
evolution
spatial
(ST)
technology
emerged
as
a
derivative
approach.
Spatial
hot
topic
field
omics
research
recent
years;
it
not
only
provides
information
on
gene
expression
levels
but
also
offers
expression.
This
shown
tremendous
potential
disease
understanding
In
this
article,
we
introduce
analytical
strategies
review
its
applications
novel
target
mechanism
unravelling.
Moreover,
discuss
current
challenges
issues
that
need
be
addressed.
conclusion,
new
perspective
Biotechnology and Bioengineering,
Год журнала:
2024,
Номер
121(4), С. 1421 - 1433
Опубликована: Янв. 15, 2024
Abstract
Acoustic
levitation,
which
allows
contactless
manipulation
of
micro‐objects
with
ultrasounds,
is
a
promising
technique
for
spheroids
formation
and
culture.
This
acoustofluidic
favors
cell–cell
interactions,
away
from
the
walls
chip,
leads
to
spontaneous
self‐organization
cells.
Using
this
approach,
we
generated
mesenchymal
stromal
cells,
hepatic
endothelial
showed
that
long‐term
culture
cells
in
acoustic
levitation
feasible.
We
also
demonstrated
its
dynamics
depended
weakly
on
parameters
but
were
strongly
dependent
levitated
cell
type.
Moreover,
spheroid
organization
was
modified
by
actin
cytoskeleton
inhibitors
or
calcium‐mediated
interaction
inhibitors.
Our
results
confirmed
rising
fundamental
research
biotechnological
industrial
application
rapidly
growing
field
microphysiological
systems.
It
allowed
easily
obtaining
specific
predictable
shape
size,
could
be
cultivated
over
several
days,
without
requiring
hydrogels
extracellular
matrix.
Bioactive Materials,
Год журнала:
2022,
Номер
18, С. 164 - 177
Опубликована: Март 19, 2022
A
personalized
medication
regimen
provides
precise
treatment
for
an
individual
and
can
be
guided
by
pre-clinical
drug
screening.
The
economical
high-efficiency
simulation
of
the
liver
tumor
microenvironment
(TME)
in
a
drug-screening
model
has
high
value
yet
challenging
to
accomplish.
Herein,
we
propose
TME
with
suspended
alginate-gelatin
hydrogel
capsules
encapsulating
patient-derived
multicellular
clusters,
culture
organoids(PDTOs)
capsule
offers
3D
matrix
environment
mechanical
biological
properties
similar
those
vivo.
As
result,
18
28
clusters
were
successfully
cultured
as
PDTOs.
These
PDTOs,
along
hepatocyte
growth
factor
(HGF)
non-cellular
components,
preserve
stromal
cells,
including
cancer-associated
fibroblasts
(CAFs)
vascular
endothelial
cells
(VECs).
They
also
maintain
stable
expression
molecular
markers
heterogeneity
original
tumors.
Drugs,
cabazitaxel,
oxaliplatin,
sorafenib,
tested
sensitivity
PDTOs
these
drugs
differs
between
individuals.
one
PDTO
oxaliplatin
was
validated
using
magnetic
resonance
imaging
(MRI)
biochemical
tests
after
clinical
corresponding
patient.
Therefore,
this
approach
is
promising
economical,
accurate,
high-throughput
screening
treatment.
Bioprinting,
Год журнала:
2022,
Номер
27, С. e00224 - e00224
Опубликована: Июль 4, 2022
Every
year,
cancer
causes
millions
of
deaths
around
the
globe,
and
researchers
have
been
engaged
to
provide
novel
therapies
for
this
threatening
disease.
However,
process
is
commonly
hampered
by
failures
in
clinical
trials.
For
reason,
efforts
made
develop
vitro
models
capable
modelling
complex
heterogeneous
tumour
microenvironment,
giving
rise
organ-on-a-chip
platforms.
These
devices
paramount
further
advancements
research
providing
a
physiologically
relevant
platform
with
organ-specificity.
Organ-on-a-chip
technology
combines
biomaterials,
tissue
engineering,
oncology,
pharmacology
one
device,
making
promising
alternative
vivo
testing.
This
work
reviews
progress
multi-organ-on-a-chip
toward
provides
an
overview
cells
used,
3D
culture
developed
materials
selected
fabricate
microfluidic
devices.
International Journal of Molecular Sciences,
Год журнала:
2023,
Номер
24(7), С. 6248 - 6248
Опубликована: Март 26, 2023
As
the
primary
site
for
biotransformation
of
drugs,
liver
is
most
focused
on
organ
type
in
pharmaceutical
research.
However,
despite
being
widely
used
research,
animal
models
have
inherent
species
differences,
while
two-dimensional
(2D)
cell
monocultures
or
co-cultures
and
three-dimensional
(3D)
monoculture
vitro
do
not
sufficiently
represent
complexity
human
liver's
structure
function,
making
evaluation
results
from
these
tools
less
reliable.
Therefore,
there
a
pressing
need
to
develop
more
representative
Fortunately,
an
exciting
new
development
recent
years
has
been
emergence
3D
co-culture
models.
These
hold
great
promise
as
research
tools,
because
they
can
reproduce
function
practically.
This
review
begins
by
explaining
main
composition
liver,
before
introducing
potential
advantages
We
also
discuss
sources
hepatocytes
methods
constructing
In
addition,
we
explore
applications
with
different
functional
states
suggest
prospects
their
further
development.
