Frontiers in Neurology,
Год журнала:
2023,
Номер
14
Опубликована: Апрель 20, 2023
Background
The
retrograde
endocannabinoid
(eCB)
pathway
is
closely
associated
with
the
etiology
of
major
depressive
disorder
(MDD)
at
both
pathophysiological
and
genetic
levels.
This
study
aimed
to
investigate
potential
role
mutations
in
eCB
underlying
mechanisms
Han
Chinese
patients
MDD.
Methods
A
total
96
drug-naïve
first-episode
MDD
62
healthy
controls
(HCs)
were
recruited.
Whole-exome
sequencing
was
performed
identify
gene
mutation
profiles
Results
filtered
focus
on
low-frequency
variants
rare
(minor
allele
frequencies
<0.05)
related
phenotypes.
Enrichment
analyses
for
146
selected
genes
examine
pathways
which
most
significant
enrichment
occurred.
protein–protein
interaction
(PPI)
network
analysis
explore
biological
functions
pathway.
Finally,
based
current
literature,
a
preliminary
conducted
effect
function
this
Our
identified
(15.02%)
depression-related
when
compared
HCs,
37
enriched
signaling
Seven
hub
mitochondrial
function,
including
Complex
I
(NDUFS4,
NDUFV2,
NDUFA2,
NDUFA12,
NDUFB11)
protein
(PARK7)
enzyme
(DLD)
regulation
oxidative
stress.
Conclusion
These
results
indicate
that
represent
etiological
factors
pathogenesis
Oxidative Medicine and Cellular Longevity,
Год журнала:
2024,
Номер
2024, С. 1 - 11
Опубликована: Май 31, 2024
The
endocannabinoid
system
is
found
throughout
the
central
nervous
system,
and
its
cannabinoids
receptor
1
critical
in
preventing
neurotoxicity
caused
by
N-methyl-D-aspartate
activation
(NMDARs).
activity
of
NMDARs
places
demands
on
endogenous
to
regulate
their
calcium
currents.
Endocannabinoids
keep
NMDAR
within
safe
limits,
protecting
neural
cells
from
excitotoxicity.
Cannabinoids
are
remembered
deliver
this
outcome
repressing
presynaptic
glutamate
discharge
or
obstructing
postsynaptic
NMDAR-managed
flagging
pathways.
must
exert
a
negative
influence
proportional
strength
signaling
for
such
control
be
effective.
goal
paper
draw
attention
towards
neuroprotective
mechanism
constituents
Cannabis
sativa
against
NMDA-induced
excitotoxic
result.
Phytochemical
investigation
cannabis
flowers
led
isolation
nine
secondary
metabolites.
A
spiro-compound,
Cannabispirenone
A,
which
treatment
prior
NMDA
exposure
significantly
increases
cell
survival
while
decreasing
ROS
production,
lipid
peroxidation,
intracellular
calcium.
Our
findings
showed
that
compound
neuroprotection
insult,
has
antioxidative
properties,
increased
cannabinoid
expression,
may
involved
pathway
neuroprotection.
The World Journal of Biological Psychiatry,
Год журнала:
2022,
Номер
24(4), С. 314 - 320
Опубликована: Авг. 11, 2022
The
endocannabinoid
system
(ECS)
is
a
critical
important
neuromodulatory
that
interacts
with
many
neurohormonal
and
neurotransmitter
systems
in
the
brain.
It
plays
pivotal
role
emotional
responses
mood
regulation.
ECS
related
psychotic
disorders,
depression,
anxiety
autism.
In
this
study,
we
aimed
to
investigate
whether
there
any
relationship
between
N-acylethanolamine
levels
bipolar
disorder.Seventy-nine
patients
disorder
diagnosis,
who
are
euthymic
period,
were
included
study.
Clinical
characteristics,
symptoms
serum
compared.
Endocannabinoid
evaluated
using
liquid
chromatography-tandem
mass
spectrometry.In
total
of
79
patients,
44
(55.69%)
females
35
(44.30%)
males.
mean
age
was
42.40
±
1.10
years.
Palmitoylethanolamide
(PEA)
higher
oleoylethanolamide
2-arachidonyl
glycerol
lower
had
at
least
one
depressive
episode
during
their
life-time
illness
than
no
while
arachidonyl
ethanolamide
unchanged.PEA
correlated
history
frequency
episodes
disorder.
Journal of Pharmaceutical and Biomedical Analysis,
Год журнала:
2023,
Номер
226, С. 115252 - 115252
Опубликована: Янв. 13, 2023
The
recent
discovery
of
N-acyl
taurines
(NATs)
as
a
class
endogenous
bioactive
lipids
and
the
perspective
their
possible
pharmacological
applications
stimulated
development
mass
spectrometry-based
methods
for
quantitative
measurements
in
biological
tissues
fluids.
We
report
here
first
time
procedure
validated
both
liver
surrogate
matrix
neat
solvent
(MeOH)
based
on
UPLC-ESI-QqQ
analysis
identification
quantification
NATs
tissue
extracts.
LC-MS
method
was
five
representative
lipid
analogues,
including
saturated,
monounsaturated
polyunsaturated
species,
namely
N-palmitoyl
taurine
(C16:0
NAT),
N-oleoyl
(C18:1
N-arachidonoyl
(C20:4
N-docosanoyl
(C22:0
NAT)
N-nervonoyl
(C24:1
evaluated
specificity,
linearity,
effect,
recovery,
repeatability
intermediate
precision
accuracy.
MeOH
by
internal
standard
approach
(d4-C20:4
showed
excellent
linearity
range
1-300
ng/ml
with
R
always
≥
0.9996
all
NATs;
intra-day
inter-day
accuracy
were
within
acceptable
range.
Specificity
assessed
NAT
standards
MeOH,
applying
confirmation
ratio
two
diagnostic
MRM
ion
transitions
product
ions
at
m/z
80
107
to
true
samples
adopted
BEH
C18
UPLC
conditions.
Limit
detection
(LOD)
limit
(LOQ)
0.3-0.4
1
ng/ml,
respectively,
compounds.
successfully
applied
assess
levels
mouse
and,
time,
varying
sections
intestine
(duodenum,
jejunum,
ileum
colon).
increased
from
duodenum
colon,
evidencing
remarkable
prevalence
large
C22:0
NAT,
typically
occurring
mainly
central
nervous
system.
These
findings
prompt
further
studies
disclose
function
various
members
this
different
peripheral
tissues.
Frontiers in Neurology,
Год журнала:
2023,
Номер
14
Опубликована: Апрель 20, 2023
Background
The
retrograde
endocannabinoid
(eCB)
pathway
is
closely
associated
with
the
etiology
of
major
depressive
disorder
(MDD)
at
both
pathophysiological
and
genetic
levels.
This
study
aimed
to
investigate
potential
role
mutations
in
eCB
underlying
mechanisms
Han
Chinese
patients
MDD.
Methods
A
total
96
drug-naïve
first-episode
MDD
62
healthy
controls
(HCs)
were
recruited.
Whole-exome
sequencing
was
performed
identify
gene
mutation
profiles
Results
filtered
focus
on
low-frequency
variants
rare
(minor
allele
frequencies
<0.05)
related
phenotypes.
Enrichment
analyses
for
146
selected
genes
examine
pathways
which
most
significant
enrichment
occurred.
protein–protein
interaction
(PPI)
network
analysis
explore
biological
functions
pathway.
Finally,
based
current
literature,
a
preliminary
conducted
effect
function
this
Our
identified
(15.02%)
depression-related
when
compared
HCs,
37
enriched
signaling
Seven
hub
mitochondrial
function,
including
Complex
I
(NDUFS4,
NDUFV2,
NDUFA2,
NDUFA12,
NDUFB11)
protein
(PARK7)
enzyme
(DLD)
regulation
oxidative
stress.
Conclusion
These
results
indicate
that
represent
etiological
factors
pathogenesis
