Abstract
Rising
antibiotic
resistance
among
periodontal
pathogens
poses
a
major
challenge
to
treatment
outcomes.
This
work
investigates
the
possible
combinatorial
potential
of
AgNPs
synthesized
using
glycyrrhizin,
natural
biosurfactant,
combat
biofilm‐forming
bacteria
isolated
from
periodontitis
patients.
In
silico
findings
revealed
that
glycyrrhizin
had
higher
binding
affinity
score
‐9.102
compared
doxycycline
(−3.599)
for
gtfB
,
plays
an
indispensable
function
in
biofilm
integrity.
were
and
optimized
utilizing
design
experiments,
considering
factors
such
as
pH,
temperature,
incubation
time
concentration
variables.
while
The
resulting
GL‐AgNPs
characterized
by
different
analytical
techniques
FTIR,
XRD,
DLS,
SEM.
nanoparticles
non‐spherical,
uniform
average
size
72
nm,
zeta
potentials
ranged
−28
mV
−
42
mV.
Antimicrobial
suggested
exhibited
significant
antibacterial
anti‐biofilm
activity
prepared
sodium
borohydride
(
p
<
0.001).
Experimental
inhibited
formation
attenuating
Gtfase
activity.
Further,
show
effective
anti‐inflammatory
with
minimal
cytotoxicity.
Overall,
this
study
highlights
inherent
antimicrobial
surfactant
properties
greatly
improve
AgNPs,
providing
promising
approach
combating
biofilm‐producing
oral
periodontitis.
ACS Applied Materials & Interfaces,
Год журнала:
2023,
Номер
15(13), С. 16329 - 16342
Опубликована: Март 22, 2023
Multidrug
resistance
in
cancer
stem
cells
(CSCs)
is
a
major
barrier
to
chemotherapy;
hence,
developing
CSC-specific
targeted
nanocarriers
for
efficient
drug
delivery
critical.
In
this
study,
monodisperse
hollow-structured
MnO2
(H-MnO2)
with
mesoporous
shell
was
created
delivery.
An
effective
therapeutic
compound
isoliquiritigenin
(ISL)
confirmed
inhibit
the
lung
stem-cell
phenotype
by
natural
screening
based
on
integrated
microfluidic
devices.
The
resultant
H-MnO2
showed
high
drug-loading
content
of
potent
CSC-targeting
ISL
and
near-infrared
fluorescent
dye
indocyanine
green
(ICG).
addition,
successively
modified
hyaluronic
acid
(HA)
enhance
targeting
CSCs
CD44
expression
levels.
H-MnO2@(ICG
+
ISL)@HA
nanocomposites
displayed
promising
chemotherapeutic
photothermal
treatment
capabilities,
as
well
NIR-triggered
release,
which
excellent
CSC-killing
effects
tumor
inhibition
efficacy.
Meanwhile,
development
effectively
restrained
phototherapy
prominent
chemotherapy
without
obvious
side
after
tail
vein
injection
vivo.
summary,
prepared
accomplished
synergistic
therapy
that
targets
CSCs,
offering
versatile
platform
diagnosis
treatment.
Current Cancer Drug Targets,
Год журнала:
2024,
Номер
24(11), С. 1089 - 1103
Опубликована: Янв. 29, 2024
Chronic
inflammation
is
associated
with
a
prolonged
increase
in
various
inflammatory
factors.
According
to
clinical
data,
it
can
be
linked
both
cancer
and
autoimmune
diseases
the
same
patients.
This
raises
critical
question
of
how
chronic
relates
seemingly
opposing
-
tumors,
which
there
immunosuppression,
diseases,
over-activation
immune
system.
In
this
review,
we
consider
as
prerequisite
for
suppression
an
increased
likelihood
damage.
We
also
discuss
potential
disease-modifying
therapies
targeting
inflammation,
helpful
autoimmunity.
On
one
hand,
pro-inflammatory
factors
persisting
areas
stimulate
production
anti-inflammatory
due
negative
feedback
loop,
eliciting
suppression.
other
bring
baseline
immunity
closer
threshold
level
required
triggering
response
using
bystander
activation
cells.
Focusing
on
role
may
open
prospects
more
intensive
drug
discovery
inflammation.
Scientific Reports,
Год журнала:
2024,
Номер
14(1)
Опубликована: Окт. 23, 2024
As
one
of
the
most
common
malignancies,
colorectal
cancer
(CRC)
usually
starts
with
a
benign
lesion
and
accumulates
DNA
damage
as
it
progresses
to
full-fledged
cancer.
Glycyrrhizin
(GL)
has
been
found
alleviate
tumor
growth
inflammation,
while
role
GL
influences
response
(DDR)
in
remains
unclear.
exposure
significantly
reduced
cell
colony
formation
viability
concomitant
increase
fragmentation
CRC,
meanwhile
induced
apoptosis
by
activating
caspase-3.
Moreover,
cycle
arrest
CRC
cells
at
S
phase,
which
was
associated
decreased
cyclin
D1
vitro.
treatment
ameliorated
promoted
DDR
vivo.
Mechanism
analysis
revealed
that
downregulated
NHEJ
pathway
via
inhibiting
HMGB1.
Finally,
expression
HMGB1
abnormal
regulated
tissue
than
adjacent
normal
tissues
TNM
stage
overall
survival.
Our
findings
indicate
may
be
novel
therapeutic
target
result
serve
promising
drug
for
treatment.
ACS Agricultural Science & Technology,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 9, 2025
AtGUS,
identified
in
the
genome
of
Aspergillus
terreus
Li-20,
can
hydrolyze
glycyrrhetinic
(GL)
into
acid
monoglucuronide
(GAMG)
and
(GA).
However,
poor
substrate
specificity
AtGUS
often
limits
its
further
application.
In
this
study,
highly
conserved
Glu416
Glu507
residues
were
as
catalytic
sites
while
Glu158,
Asp163,
Arg565,
which
form
hydrogen
bonds
with
substrate,
play
critical
roles
enzymatic
activity,
Arg565
being
particularly
important.
When
on
loop
8
surface
TIM
barrel
was
mutated
to
glutamate,
GAMG
became
sole
product,
yield
from
GL
conversion
up
85%.
hydrolysis
reaction
terminated,
7.32-fold
higher
than
that
wild
enzyme.
Molecular
docking
dynamic
simulation
analyses
revealed
affinity
for
mutant
R565E
(Km
=
0.192
mmol/L)
improved,
but
reduced
efficiency
toward
(kcat
5.515
±
0.07
mmol–1
L
s–1).
This
study
provides
new
insights
understanding
mechanism
ideas
efficient
preparation
GAMG.
Oncology Letters,
Год журнала:
2025,
Номер
29(4), С. 1 - 10
Опубликована: Март 4, 2025
The
objective
of
the
present
study
was
to
elucidate
mechanism
by
which
glycyrrhizin
enhances
antitumor
activity
cisplatin
in
non-small
cell
lung
cancer.
Initially,
A549
cells
were
treated
with
different
concentrations
(0.25-8
mM)
or
(10-160
µM)
for
48
h
investigate
effect
combined
on
vitro.
Subsequently,
divided
into
control
(untreated),
CP
(20
µM
cisplatin),
GL
(2
mM
glycyrrhizin)
and
+
2
groups
underlying
glycyrrhizin.
After
incubation,
viability
colony-forming
ability
assessed
using
MTT
colony
formation
assays.
Apoptosis
levels
cycle
progression
analyzed
flow
cytometry
western
blotting
used
evaluate
apoptosis-
cycle-related
proteins.
Additionally,
comet
assays
DNA
damage
relevant
results
demonstrated
both
individually
reduced
a
concentration-dependent
manner.
Cisplatin
lower
half-maximal
inhibitory
concentration
(IC50)
at
higher
concentrations,
an
IC50
value
~35
Furthermore,
treatment
synergistically
ability,
induced
apoptosis
arrested
G2
phase,
showing
greater
efficacy
when
compared
either
individually.
In
addition,
analysis
that,
comparison
alone,
markedly
increased
protein
expression
B-cell
lymphoma
2-associated
X
protein,
cleaved-caspase-3/caspase-3,
γH2AX,
phosphorylated-checkpoint
kinase
1
phosphorylated-p53/p53,
while
notably
reducing
2,
cyclin
D1,
cyclin-dependent
4.
findings
indicate
that
cancer
modulating
apoptosis.
