Journal of Nanobiotechnology,
Год журнала:
2025,
Номер
23(1)
Опубликована: Март 17, 2025
Circular
RNAs
(circRNAs),
constituting
a
novel
class
of
endogenous
non-coding
generated
through
the
reverse
splicing
mRNA
precursors,
possess
capacity
to
regulate
gene
transcription
and
translation.
Recently,
pivotal
role
circRNAs
in
controlling
vascular
aging,
as
well
pathogenesis
progression
aging-related
diseases,
has
garnered
substantial
attention.
Vascular
aging
plays
crucial
increased
morbidity
mortality
elderly.
Endothelial
cells
(ECs)
smooth
muscle
(VSMCs)
are
components
intima
media
layers
wall,
respectively,
closely
involved
mechanisms
underlying
diseases.
The
review
aims
provide
comprehensive
exploration
connection
between
Besides,
circRNAs,
potential
diagnostic
markers
or
therapeutic
targets
for
will
be
discussed
thoroughly,
along
with
challenges
limitations
their
clinical
application.
Investigating
molecular
diseases
insight
into
early
diagnosis
therapy,
even
effective
prognosis
assessment
these
conditions.
Journal of Experimental & Clinical Cancer Research,
Год журнала:
2025,
Номер
44(1)
Опубликована: Апрель 2, 2025
Abstract
Background
Lung
cancer
is
a
serious
threat
to
human
life
and
health,
but
effective
screening
treatment
methods
are
lacking.
Circular
RNAs
(circRNAs)
have
important
biological
functions
closely
related
tumour
development.
Some
studies
shown
that
the
8-oxo-7,8-dihydroguanosine
(o8G)
modification
plays
key
role
in
disease
process,
effect
of
o8G
on
circRNAs
has
not
been
elucidated.
Moreover,
cuproptosis
novel
mode
cell
death
which
copper
ions
directly
promote
protein
aggregation
disruption
cellular
metabolic
pathways.
The
present
study
revealed
circKIAA1797
occurs
promotes
lung
development
by
inhibiting
cuproptosis,
provides
new
perspectives
for
epitranscriptomic
therapeutic
approaches
cancer.
Methods
circRNA
differential
expression
profiles
were
via
RNA
high-throughput
sequencing,
lines
tissues
was
detected
using
qPCR.
Experiments
such
as
immunoprecipitation
(o8G
RIP)
crosslinking
(CLIP)
performed
explore
presence
circKIAA1797.
regulation
reader
Y-box
binding
1
(YBX1)
explored
nuclear–cytoplasmic
fractionation,
actinomycin
D
(Act
D)
stability
experiments
other
experiments.
silencing
overexpression
systems
constructed
vivo
vitro
Tagged
affinity
purification
(TRAP),
(RIP),
coimmunoprecipitation
(Co-IP),
immunofluorescence
(IF)
staining
subsequently
conducted
reveal
molecular
mechanism
regulates
Results
This
first
YBX1
recognises
ROS-induced
modifications
increases
cytoplasmic
circKIAA1797,
associated
with
stage
prognosis,
significantly
function
both
vitro.
inhibits
intracellular
ferredoxin
(FDX1)
mRNA,
decreasing
FDX1
mRNA
stability,
expression,
signal
transducer
activator
transcription
(STAT1)
lipoyltransferase
(LIPT1)
transcription;
moreover,
closure
mitochondrial
permeability
transition
pore
(mPTP),
ultimately
Conclusions
an
can
inhibit
proteins
promoting
mPTP
closure,
only
theoretical
basis
in-depth
understanding
mechanisms
also
potential
target
treatment.
Investigative Ophthalmology & Visual Science,
Год журнала:
2025,
Номер
66(4), С. 26 - 26
Опубликована: Апрель 11, 2025
Lymphangiogenesis
plays
important
roles
in
the
pathogenesis
of
human
diseases,
including
inflammatory
ocular
metabolic
and
cancers,
by
affecting
lipid
metabolism
immune
homeostasis.
Despite
growing
evidence
showing
that
circular
RNAs
(circRNAs)
act
as
regulators
pathways,
their
lymphatic
dysfunction
remain
unclear.
This
study
aims
to
explore
involvement
circRNA-KIF6
(cKIF6)
pathological
lymphangiogenesis
elucidate
underlying
mechanism.
The
cKIF6
expression
was
evaluated
mouse-sutured
corneas
endothelial
cells
(LECs)
isolated
from
juvenile
foreskin
under
conditions.
Functional
assays,
viability,
proliferation,
migration,
tube
formation,
were
conducted
on
LECs
after
silencing.
using
cornea
Matrigel
plug
models.
Mechanistic
studies
explored
role
a
molecular
sponge
for
miR-582
its
downstream
effect
methylsterol
monooxygenase
1
(MSMO1).
significantly
elevated
inflamed
LECs.
Silencing
impaired
LEC
leading
reduced
both
vitro
vivo
Mechanistically,
acted
sponge,
resulting
MSMO1
increased
cholesterol
content
augmented
formation
abilities
cKIF6-overexpressing
attenuated
inhibitor
biosynthesis.
regulates
targeting
metabolism,
making
it
promising
therapeutic
target
lymphangiogenesis-related
diseases.
