Breast cancer spheroids prefer activated macrophages as an accomplice: An in vitro study DOI
Abhishek Teli,

Ranjani Iyer,

Karthik Shanbhag

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Сен. 25, 2024

Abstract Cancer, a heterogeneous disease in nature, often requires help from diverse pro-tumor or tumor-associated- cells, which are recruited and persevered within the stroma. Pro-tumor stromal cells provide essential support needed for tumor growth, metastasis, development of drug resistance due time. Tumor-associated macrophages, one such to microenvironment survival. In recent years, TAMs have been identified as potential targets therapeutic agents, encourages in-depth characterization their crosstalk with tumors. The current study has successfully developed cost-effective vitro platform Chemokine Assisted Recruitment Macrophages spheroids mimicking physiology TAM recruitment. Firstly, monocytic cell line (U937) were converted into activated naive macrophages (M0) pro-and anti-inflammatory (M1 M2) subtypes. Monocytes, M0, M1, M2 characterized extensively. Secondly, polarized subjected chemokine-dependent recruitment monotypic heterotypic breast cancer spheroids. nature is further investigated by assessing profile chemokines chemokine receptors. Recruited also observed manipulate spheroid behavior many ways. exhibit an increased level Siglec-1 (CD169), markers. platform’s application can be extended understand process other immune/stromal solid It could addition arrays platforms screen efficiency cell-based immunotherapeutics future.

Язык: Английский

Noncoding RNA-encoded peptides in cancer: biological functions, posttranslational modifications and therapeutic potential DOI Creative Commons

Shiming Tan,

Wenjuan Yang, Zongyao Ren

и другие.

Journal of Hematology & Oncology, Год журнала: 2025, Номер 18(1)

Опубликована: Фев. 19, 2025

In the present era, noncoding RNAs (ncRNAs) have become a subject of considerable scientific interest, with peptides encoded by ncRNAs representing particularly promising avenue investigation. The identification ncRNA-encoded in human cancers is increasing. These regulate cancer progression through multiple molecular mechanisms. Here, we delineate patterns diverse and provide synopsis methodologies employed for that possess capacity to encode these peptides. Furthermore, discuss impacts on biological behavior cells underlying conclusion, describe prospects challenges need be overcome.

Язык: Английский

Процитировано

0

Effects of circulating RNAs on tumor metabolism in lung cancer (Review) DOI Open Access

Pengfei Zhao,

Zhengfeng Zhu, Xi Zheng

и другие.

Oncology Letters, Год журнала: 2025, Номер 29(4), С. 1 - 11

Опубликована: Фев. 27, 2025

During the development and progression of lung cancer, cell metabolism function is altered. Thus, cells rely on aerobic glycolysis abnormal lipid amino acid to obtain energy nutrients for growth, proliferation drug resistance. Circular RNAs (circRNAs), a class non-coding RNAs, serve important biological roles in growth tumors. Functionally, circRNAs act as molecular sponges that absorb microRNAs (miRNAs) RNA-binding proteins protein scaffolds regulate gene transcription translation through maintenance mRNA stability. In addition, are regulators tumor promote mediating proliferation, metastasis induction chemoresistance. Results previous studies reveal may key role regulating metabolic processes miRNA sponging alternative mechanisms. demonstrate potential therapeutic targets cancer. The present study aimed review effects cancer provide novel insights into clinical treatment also theoretical basis targets.

Язык: Английский

Процитировано

0

EVA1B facilitates esophageal squamous carcinoma progression and recruitment of immunosuppressive myeloid-derived suppressor cells in the tumor microenvironment DOI Creative Commons
Tian Xia, Yongkang Zhang,

Haodong Peng

и другие.

Pharmacological Research, Год журнала: 2024, Номер unknown, С. 107521 - 107521

Опубликована: Ноя. 1, 2024

Язык: Английский

Процитировано

2

Breast cancer spheroids prefer activated macrophages as an accomplice: An in vitro study DOI
Abhishek Teli,

Ranjani Iyer,

Karthik Shanbhag

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Сен. 25, 2024

Abstract Cancer, a heterogeneous disease in nature, often requires help from diverse pro-tumor or tumor-associated- cells, which are recruited and persevered within the stroma. Pro-tumor stromal cells provide essential support needed for tumor growth, metastasis, development of drug resistance due time. Tumor-associated macrophages, one such to microenvironment survival. In recent years, TAMs have been identified as potential targets therapeutic agents, encourages in-depth characterization their crosstalk with tumors. The current study has successfully developed cost-effective vitro platform Chemokine Assisted Recruitment Macrophages spheroids mimicking physiology TAM recruitment. Firstly, monocytic cell line (U937) were converted into activated naive macrophages (M0) pro-and anti-inflammatory (M1 M2) subtypes. Monocytes, M0, M1, M2 characterized extensively. Secondly, polarized subjected chemokine-dependent recruitment monotypic heterotypic breast cancer spheroids. nature is further investigated by assessing profile chemokines chemokine receptors. Recruited also observed manipulate spheroid behavior many ways. exhibit an increased level Siglec-1 (CD169), markers. platform’s application can be extended understand process other immune/stromal solid It could addition arrays platforms screen efficiency cell-based immunotherapeutics future.

Язык: Английский

Процитировано

0