Integrated proteomics analysis and network pharmacology to elucidate the mechanism of Zhilong Huoxue Tongyu Capsule alleviate hypertensive retinopathy in Ang II infusion mice model DOI Creative Commons
Jiao Wu, Wen Xie, Yucen Xie

и другие.

Frontiers in Pharmacology, Год журнала: 2025, Номер 16

Опубликована: Май 21, 2025

Zhilong Huoxue Tongyu Capsule (ZLHXTY) has been used in clinical treatment of vascular diseases caused by hypertension over 20 years. However, the specific mechanisms ZLHXTY alleviate hypertensive retinopathy (HR) needs to be further explored. HR mouse model was established infusing Ang II via subcutaneously implanted osmotic mini-pumps, followed oral administration (0.35, 0.7, 1.4 g/kg/day) 28 days for treatment. To assess impacts on retinal neurodegeneration and injury, multiple experiments such as OCTA, ERG HE staining were performed. Subsequently, network pharmacological 4D-label-free proteomics clarify potential targets alleviated HR. Finally, Western blot, ELISA, IF, other techniques utilized detect expression proteins related inflammation, oxidative stress, NLRP3 inflammasome activation. significantly dysfunction, increased blood flow, mitigated pathological changes tissues edema mice. Network pharmacology indicated that might exert anti-inflammatory anti-oxidative stress effects through TNF NF-κB. Proteomic analysis showed differential between ZL group mainly enriched immune-inflammatory response, main mechanism which assembly inflammasome. Subsequent vivo corroborated remarkably attenuated inflammation damage tissues. Further demonstrated inhibited NLRP3/Caspase-1/GSDMD signaling pathway protein expression. TEM results also verified pyroptosis cells. Our suggest regulating axis, inhibiting pyroptosis, thereby relieving dysfunction injury

Язык: Английский

The Role of Fractalkine in Diabetic Retinopathy: Pathophysiology and Clinical Implications DOI Open Access

Cheng-Yung Lee,

Chang‐Hao Yang

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(1), С. 378 - 378

Опубликована: Янв. 4, 2025

Diabetic retinopathy (DR) is a complication of diabetes, characterized by progressive microvascular dysfunction that can result in vision loss. Chronic hyperglycemia drives oxidative stress, endothelial dysfunction, and inflammation, leading to retinal damage complications such as neovascularization. Current treatments, including anti-VEGF agents, have limitations, necessitating the exploration alternative therapeutic strategies. Fractalkine (CX3CL1), chemokine with dual roles membrane-bound adhesion molecule soluble chemoattractant, has emerged potential target. Its receptor, CX3CR1, expressed on immune cells mediates processes cell recruitment microglial activation through intracellular signaling pathways. In DR, fractalkine plays critical angiogenesis, neuroprotection, balancing tissue repair. elevated levels are associated inflammation dysfunction. Experimental studies suggest deficiency exacerbates severity diabetic (DR), whereas exogenous appears reduce neuronal damage. However, its role pathological angiogenesis within DR remains unclear warrants further investigation. Preclinical evidence indicates may hold potential, particularly mitigating injury early-stage DR.

Язык: Английский

Процитировано

1
Integrated proteomics analysis and network pharmacology to elucidate the mechanism of Zhilong Huoxue Tongyu Capsule alleviate hypertensive retinopathy in Ang II infusion mice model DOI Creative Commons
Jiao Wu, Wen Xie, Yucen Xie

и другие.

Frontiers in Pharmacology, Год журнала: 2025, Номер 16

Опубликована: Май 21, 2025

Zhilong Huoxue Tongyu Capsule (ZLHXTY) has been used in clinical treatment of vascular diseases caused by hypertension over 20 years. However, the specific mechanisms ZLHXTY alleviate hypertensive retinopathy (HR) needs to be further explored. HR mouse model was established infusing Ang II via subcutaneously implanted osmotic mini-pumps, followed oral administration (0.35, 0.7, 1.4 g/kg/day) 28 days for treatment. To assess impacts on retinal neurodegeneration and injury, multiple experiments such as OCTA, ERG HE staining were performed. Subsequently, network pharmacological 4D-label-free proteomics clarify potential targets alleviated HR. Finally, Western blot, ELISA, IF, other techniques utilized detect expression proteins related inflammation, oxidative stress, NLRP3 inflammasome activation. significantly dysfunction, increased blood flow, mitigated pathological changes tissues edema mice. Network pharmacology indicated that might exert anti-inflammatory anti-oxidative stress effects through TNF NF-κB. Proteomic analysis showed differential between ZL group mainly enriched immune-inflammatory response, main mechanism which assembly inflammasome. Subsequent vivo corroborated remarkably attenuated inflammation damage tissues. Further demonstrated inhibited NLRP3/Caspase-1/GSDMD signaling pathway protein expression. TEM results also verified pyroptosis cells. Our suggest regulating axis, inhibiting pyroptosis, thereby relieving dysfunction injury

Язык: Английский

Процитировано

0