Drug and Chemical Toxicology,
Год журнала:
2024,
Номер
unknown, С. 1 - 8
Опубликована: Янв. 30, 2024
Wuzhuyu
decoction
(WZYD)
is
a
well-known
classic
traditional
Chinese
medicine
prescription
and
has
been
widely
used
to
treat
headache,
nausea,
vomiting,
insomnia,
etc.
However,
little
published
information
available
about
its
safety.
Our
aim
was
investigate
the
acute
subacute
oral
toxicity
of
WZYD
extract
in
rats
following
technical
guidelines
from
China's
National
Medical
Products
Administration
(NMPA)
for
single
repeated
doses
studies
drugs.
Acute
assessed
via
administration
at
4
g/kg
three
times
within
day
followed
by
14-day
observation
period.
To
evaluate
toxicity,
were
orally
administered
with
0,
0.44,
1.33,
28
days.
The
items
examined
included
clinical
signs,
body
weight,
food
consumption,
hematological
biochemical
parameters,
bone
marrow
smear,
organ
index,
histopathology.
After
12
(3
×
g/kg)
extract,
no
mortality
toxic
effects
observed
during
In
study,
did
not
cause
any
significant
treatment-related
abnormality
each
item
rats,
so
adverse
effect
level
(NOAEL)
days
considered
be
g/kg,
which
approximately
80-fold
proposed
dosage.
Frontiers in Pharmacology,
Год журнала:
2024,
Номер
14
Опубликована: Янв. 8, 2024
Background:
Gastric
ulcers
(GUs)
are
prevalent
digestive
disorders
worldwide.
Wuzhuyu
Decoction
(WZYT)
is
a
traditional
Chinese
medicine
that
has
been
employed
for
centuries
to
alleviate
ailments
like
indigestion
and
vomiting.
This
study
aims
explore
the
potential
effects
underlying
mechanisms
of
WZYT
on
alcohol
induced
gastric
ulcer
treatment.
Methods:
We
macroscopic
assessment
evaluate
index
(UI),
while
enzyme-linked
immunosorbent
assay
(ELISA)
was
utilized
detecting
biochemical
indicators.
Pathological
tissue
analysis
involved
hematoxylin-eosin
(H&E)
staining
Periodic
Acid-Schiff
(PAS)
assess
damage.
Additionally,
integration
network
metabolomics
facilitated
prediction
targets.
Validation
conducted
using
Western
blotting.
Results:
The
research
revealed
treatment
significantly
reduced
(UI)
regulation
alcohol-induced
indicators
levels.
improvements
were
observed
in
pathological
tissue.
Network
results
indicated
62
compounds
contained
modulate
by
regulating
183
genes.
serum
significant
changes
content
19
metabolites
after
Two
pivotal
targets,
heme
oxygenase
1
(HMOX1)
albumin
(ALB),
believed
assume
role
construction
“compounds-target-metabolite”
networks.
blot
confirmed
capacity
elevate
expression
HMOX1
ALB
Conclusion:
provides
scientific
basis
propel
clinical
use
GUs.
Our
theoretical
decoction
ulcers.
ACS Nano,
Год журнала:
2024,
Номер
18(34), С. 23684 - 23701
Опубликована: Авг. 19, 2024
The
nanodrug
delivery
system-based
nasal
spray
(NDDS-NS)
can
bypass
the
blood-brain
barrier
and
deliver
drugs
directly
to
brain,
offering
unparalleled
advantages
in
treatment
of
central
nervous
system
(CNS)
diseases.
However,
current
design
NNDS-NS
is
excessively
focused
on
mucosal
absorption
while
neglecting
impact
deposition
nose-to-brain
drug
delivery,
resulting
an
unsatisfactory
efficiency.
In
this
study,
effect
dispersion
medium
viscosity
NDDS-NS
was
elucidated.
optimized
formulation
F5
(39.36
mPa·s)
demonstrated
significantly
higher
olfactory
fraction
(ODF)
23.58%,
a
strong
correlation
between
ODF
intracerebral
(
Cells,
Год журнала:
2022,
Номер
11(15), С. 2444 - 2444
Опубликована: Авг. 6, 2022
Migraines
constitute
a
common
neurological
and
headache
disorder
affecting
around
15%
of
the
world’s
population.
In
addition
to
other
mechanisms,
neurogenic
neuroinflammation
has
been
proposed
play
part
in
migraine
chronification,
which
includes
peripheral
central
sensitization.
There
is
therefore
considerable
evidence
suggesting
that
inflammation
intracranial
meninges
could
be
key
element
calcitonin
gene-related
peptide
(CGRP),
leading
sensitization
trigeminal
meningeal
nociceptors
migraines.
are
several
studies
have
utilized
this
approach,
with
strong
focus
on
using
inflammatory
animal
models.
Data
from
these
show
process
involves
trigeminovascular
afferent
nerve
terminals.
Further,
by
applying
wide
range
different
pharmacological
interventions,
insight
gained
pathways
involved.
Importantly,
we
discuss
how
models
should
used
care
it
important
evaluate
outcomes
light
pathology.
International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(12), С. 6655 - 6655
Опубликована: Июнь 17, 2024
Migraine
is
a
common
and
debilitating
neurological
disorder
characterized
by
the
recurrent
attack
of
pulsating
headaches
typically
localized
on
one
side
head
associated
with
other
disabling
symptoms,
such
as
nausea,
increased
sensitivity
to
light,
sound
smell
mood
changes.
Various
clinical
factors,
including
excessive
use
migraine
medication,
inadequate
acute
treatment
stressful
events,
can
contribute
worsening
condition,
which
may
evolve
chronic
migraine,
that
is,
headache
present
>15
days/month
for
at
least
3
months.
Chronic
frequently
various
comorbidities,
anxiety
disorders,
particularly
depression,
complicate
prognosis,
response
overall
outcomes.
Emerging
research
indicates
connection
between
alterations
in
composition
gut
microbiota
mental
health
conditions,
are
considered
disorders
gut-brain
axis.
This
underscores
potential
modulating
new
avenue
managing
these
conditions.
In
this
context,
it
interesting
investigate
whether
its
form,
exhibits
dysbiosis
profile
similar
observed
individuals
depression.
could
pave
way
interventions
aimed
treating
difficult-to-manage
migraines.
Frontiers in Pharmacology,
Год журнала:
2025,
Номер
16
Опубликована: Янв. 29, 2025
Euodiae
Fructus
(EF)
is
the
dried
and
nearly
ripe
fruit
of
Euodia
rutaecarpa
,
first
recorded
in
Shen
Nong’s
Herbal
Classic
.
EF
a
versatile
Traditional
Chinese
Medicine
(TCM)
known
for
effects
dispelling
colds
alleviating
pain,
suppressing
adverse
qi
to
relieve
vomiting,
boosting
yang
mitigate
diarrhea.
However,
it
should
be
noted
that
possesses
mild
toxicity.
In
TCM
prescriptions,
employed
treat
various
ailments,
including
abdominal
diarrhea,
chronic
non-atrophic
gastritis,
irritable
bowel
syndrome,
primary
dysmenorrhea.
This
review
collected
literature
published
before
September
2024
on
EF.
An
exhaustive
analysis
was
conducted
utilizing
multiple
sources,
namely
classic
books
scientific
databases
like
Web
Science,
PubMed,
Elsevier,
ACS,
ResearchGate,
Google
Scholar,
National
Knowledge
Infrastructure.
So
far,
more
than
300
metabolites
have
been
extracted
identified
from
EF,
exhibiting
pharmacological
effects,
such
as
cardiovascular
protection,
gastrointestinal
neuroprotection,
anti-inflammation,
analgesia,
anti-tumor,
glucose
lipid
metabolism
regulation,
etc.
It
also
exhibits
diverse
toxicological
properties
poses
specific
toxic
risks
liver,
heart,
kidney.
Nonetheless,
research
scarce
regarding
toxicology
especially
its
cardiotoxicity
nephrotoxicity.
Further
in-depth
necessary
explore
mechanisms
underlying
EF’s
develop
strategies
quality
control
toxicity
mitigation.
The
can
reduced
by
processing,
but
this
aspect
rarely
discussed,
needs
further
standardized.
Evodiamine,
rutaecarpine,
limonin
are
effective
one
causes
evodiamine
rutaecarpine
intensely
studied,
there
few
studies
other
Therefore,
paper
focuses
botanical
characteristics,
traditional
applications,
processing
methods,
phytochemistry,
control,
pharmacology,
We
hope
provides
theoretical
basis
future
high-value
high-connotation
development
World Journal of Traditional Chinese Medicine,
Год журнала:
2025,
Номер
11(1), С. 45 - 57
Опубликована: Янв. 1, 2025
Objective:
The
objective
of
the
study
was
to
explore
mechanism
action
Wuzhuyu
decoction
(WZYD)
in
treating
chronic
atrophic
gastritis
(CAG).
Materials
and
Methods:
Intersectional
target
genes
WZYD,
CAG,
their
differential
metabolites
were
obtained.
Search
Tool
for
Retrieval
Interacting
Genes/Proteins
(STRING)
used
construct
a
protein–protein
interaction
network
targets,
critical
WZYD
treatment
CAG
screened.
Functional
pathway
enrichment
analyses
performed
complete
network.
Pathological
tests
enzyme-linked
immunosorbent
assay
(ELISA)
kits
detect
related
factors.
Cell
apoptosis
evaluated
using
Annexin
V/propidium
iodide
staining
method,
followed
by
flow
cytometry.
expression
proteins
associated
with
phosphoinositide
3-kinase
(PI3K)/protein
kinase
B
(AKT)
signaling
downstream
detected
Western
Blotting.
Results:
In
total,
28
intersection
targets
core
pathways
identified.
PI3K/AKT
status
selected
validate
rats.
Cytokines,
PI3K
expression,
factors
downregulated
after
intervention.
Conclusions:
may
protect
against
N-methyl-N’-nitro-N-nitrosoguanidine-induced
through
pathway.
Pharmaceuticals,
Год журнала:
2025,
Номер
18(3), С. 426 - 426
Опубликована: Март 18, 2025
Background:
Chronic
migraine
(CM)
is
a
common
complex
nervous
system
disease,
often
accompanied
by
symptoms
of
the
digestive
tract
that
interact
with
each
other,
leading
to
prolonged
and
difficult-to-cure
migraines.
These
are
associated
abnormalities
in
5-HT
its
receptors.
Wuzhuyu
decoction
(WZYD)
traditional
Chinese
medicine
prescription
commonly
used
clinics
treat
CM;
it
relieves
gastrointestinal
symptoms,
such
as
nausea
vomiting;
however,
mechanism
still
unclear.
Investigating
differences
role
WZYD
compared
existing
drugs
targeting
receptors
treatment
CM
not
only
helps
elucidate
pathogenesis
but
also
provides
possibilities
for
development
new
therapeutic
approaches.
Methods:
An
inflammation
soup
(IS)-induced
male
rat
model
was
established.
Based
on
preliminary
experiment,
target
treating
determined
network
pharmacology,
verified
molecular
docking.
ELISA,
immunofluorescence,
western
blot,
real-time
quantitative
polymerase
chain
reaction
(RT-qPCR)
were
evaluate
expression
levels
CM-related
indicators
(5-HT,
calcitonin
gene-related
peptide
(CGRP),
c-Fos)
ensure
successful
establishment
effectiveness
drug.
On
this
basis,
protein
5-HT1A/3A
their
cAMP-response
element
binding
(CREB)
signaling
pathway
detected
blot
immunohistochemistry.
The
validated
using
5-HT1A
receptor
antagonist
(WAY
100635)
5-HT3A
agonist
(SR
57227).
Results:
results
showed
increased
brain,
decreased
CGRP,
c-Fos,
ionized
calcium-binding
adapter
molecule
1
(Iba1),
relieved
CM.
At
same
time,
brain
colon
rats.
Subsequently,
further
exerted
brain-gut
integrated
effects
regulating
CREB
mediated
Conclusions:
regulates
neurotransmitters
at
specifically
colon,
which
explains
characteristics
advantages
from
perspective.
While
effectively
relieving
headache
improves
related
more
conducive
