Cervical
carcinoma
poses
a
significant
health
threat,
with
traditional
treatments
proving
inadequate
in
advanced
stages.
Curcumin,
bioactive
compound
derived
from
turmeric,
exhibits
notable
anti-inflammatory,
antioxidant,
and
antineoplastic
properties,
potentially
modulating
autophagy,
metastasis
cancer
cells.
This
study
examines
curcumin's
impact
on
autophagy
cervical
carcinoma,
focusing
its
interaction
autophagy-related
gene
3
(ATG3).
SiHa
HeLa
cell
lines
were
treated
curcumin,
ATG3
knockdown
(shATG3),
their
combination.
Cell
migration
was
evaluated
via
wound
healing
assays,
while
proliferation
CCK-8
assays.
LC3
expression
assessed
using
immunofluorescence
western
blotting.
Molecular
docking
simulations
identified
binding
interactions
key
proteins.
Curcumin
shATG3
significantly
inhibited
both
proliferation,
synergistic
effect
observed
when
combined.
enhanced,
indicating
increased
autophagy.
Docking
studies
revealed
potential
to
MMP2,
MMP9,
TGF-β,
ATG3,
LC3,
p62,
suggesting
modulation
of
these
pathways.
The
combination
curcumin
also
enhancing
supporting
the
as
therapeutic
agent
for
carcinoma.
Further
clinical
research
is
needed
validate
findings.
Frontiers in Immunology,
Год журнала:
2023,
Номер
14
Опубликована: Окт. 31, 2023
Antimicrobial
photodynamic
therapy
(aPDT)
has
become
a
potent
contender
in
the
fight
against
microbial
infections,
especially
context
of
rising
antibiotic
resistance
crisis.
Recently,
there
been
significant
interest
polyphenolic
natural
products
as
potential
photosensitizers
(PSs)
aPDT,
given
their
unique
chemical
structures
and
inherent
antimicrobial
properties.
Polyphenolic
products,
abundant
readily
obtainable
from
sources,
are
generally
regarded
safe
highly
compatible
with
human
body.
This
comprehensive
review
focuses
on
latest
developments
future
implications
using
polyphenols
PSs
aPDT.
Paramount
compounds,
including
curcumin,
hypericin,
quercetin,
hypocrellin,
celastrol,
riboflavin,
resveratrol,
gallic
acid,
aloe
emodin,
elaborated
upon
respect
to
structural
characteristics,
absorption
properties,
effects.
Furthermore,
aPDT
mechanism,
specifically
its
targeted
action
cells
biofilms,
is
also
discussed.
demonstrate
immense
representing
promising
alternate
approach
counteract
antibiotic-resistant
bacteria
biofilm-related
infections.
Journal of Nanobiotechnology,
Год журнала:
2023,
Номер
21(1)
Опубликована: Сен. 4, 2023
Intervertebral
disc
degeneration
(IVDD)
is
a
major
contributor
to
spinal
disorders.
Previous
studies
have
indicated
that
the
infiltration
of
immunocytes,
specifically
macrophages,
plays
crucial
role
in
advancement
IVDD.
Exosomes
(exo)
are
believed
play
significant
intercellular
communication.
This
study
aims
investigate
exosomes
derived
from
degenerated
nucleus
pulposus
(dNPc)
process
macrophages
M1
polarization.Nucleus
(NP)
tissue
and
cells
(NPc)
were
collected
patients
with
intervertebral
idiopathic
scoliosis.
Immunohistochemistry
analysis
was
performed
determine
number
NP
tissue.
Subsequently,
(dNPc-exo)
non-degenerated
(nNPc-exo)
co-cultured
M0
which
induced
THP-1
cells.
The
phenotype
assessed
using
western
blot,
flow
cytometry,
immunofluorescence
staining,
qRT-PCR.
RNA-sequencing
conducted
examine
expression
levels
microRNAs
dNPc-exo
nNPc-exo
groups,
qRT-PCR
effect
pf
different
microRNA
induce
macrophage
polarization.
Furthermore,
blot
employed
demonstrate
regulatory
carried
by
on
downstream
target
signaling
pathways
macrophages.
Finally,
an
animal
model
IVDD
utilized
impact
inducing
polarization
its
process.In
this
study,
we
observed
increase
as
(IVD)
degraded.
Additionally,
discovered
dNPc
releases
could
promote
towards
phenotype.
Notably,
through
identified
miR-27a-3p
highly
expressed
miRNA
group,
significantly
influences
induction
And
then,
has
ability
transport
PPARγ/NFκB/PI3K/AKT
pathway,
thereby
influencing
We
experiments
rat
carrying
actually
exacerbated
degradation
IVD.In
conclusion,
our
findings
highlight
provide
basis
for
further
investigation
into
mechanism
potential
exosome-based
therapy.
The American Journal of Chinese Medicine,
Год журнала:
2024,
Номер
52(04), С. 987 - 1011
Опубликована: Янв. 1, 2024
Triple
negative
breast
cancer
(TNBC)
is
an
aggressive
subtype
of
cancer.
Conventional
treatment
options
for
TNBC
often
have
limited
efficacy
and
significant
side
effects.
In
recent
years,
traditional
Chinese
medicine
(TCM)
has
shown
promising
results
in
the
TNBC.
TCMs
include
herb
combinations
that
synergistic
effects
to
regulate
homeostasis
body,
reduce
tumor
resistance,
improve
patient
quality
life.
At
present,
three
main
TCM
methods
are
used
treat
clinic:
strengthening
body’s
dispelling
phlegm,
removing
toxins.
This
paper
reviews
theories
mechanisms
each
treatment.
The
method
resistance
emphasizes
enhancing
original
Qi
fight
against
pathogenic
factors;
phlegm
seeks
eliminate
stagnation
alleviate
burden
on
affected
organs;
toxins
focuses
detoxification
detumescence
remove
toxic
elements
associated
with
Although
these
from
different
etiologies,
they
achieved
good
therapeutic
represent
important
academic
approach:
That
is,
cure
disease
a
comprehensive
view
body
restore
balance
Yin
Yang.
knowledge
lays
foundation
future
development
reasonable
application
clinic.
Biochemical Pharmacology,
Год журнала:
2024,
Номер
227, С. 116456 - 116456
Опубликована: Июль 28, 2024
Furanodienone,
a
biologically
active
constituent
of
sesquiterpenes
isolated
from
Rhizome
Curcumae,
has
been
reported
to
induce
apoptosis
in
human
colorectal
cancer
(CRC)
cells
by
promoting
the
generation
reactive
oxygen
species
(ROS).
However,
source
ROS
and
how
it
manipulates
CRC
remains
be
elucidated.
Herein,
we
assessed
potential
role
well-known
sources
intracellular
ROS-mitochondrial
electron
transport
chain
nicotinamide
adenine
dinucleotide
phosphate
oxidases
(NOXs),
on
furanodienone-induced
cell
death.
The
results
indicated
that
furanodienone
substantially
increased
levels
mitochondrial
ROS,
which
were
subsequently
eliminated
general
NOX
inhibitor.
Specifically,
nuclear
factor
kappa-B
(NF-κB)
translocation
triggered
significant
rise
expression
NOX4,
an
isoform
NOXs
family,
upon
treatment.
Nevertheless,
specific
NOX4
inhibitor
GLX351322
attenuated
production.
As
result,
burst
induced
suppressed
peroxiredoxin1
(PRDX1),
redox
signaling
protein
overexpressed
cells,
through
factor-erythroid-2-related
2
(Nrf2)-dependent
pathway,
thus
amplifying
mitogen-activated
kinases
(MAPKs)/p53-mediated
apoptotic
increasing
p-p38,
p-JNK
levels,
as
well
cleaved
caspases
-3,
-8
-9.
In
vivo
experiments
further
confirmed
anti-proliferative
impact
PRDX1
following
summary,
study
demonstrated
is
initiated
derived
targeted
activated
downstream
MAPKs/p53-mediated
caspase-dependent
pathway.
Our
findings
may
provide
novel
insights
into
development
adjuvant
drugs
for
treatment
therapeutic
applications.
Frontiers in Pharmacology,
Год журнала:
2024,
Номер
15
Опубликована: Авг. 6, 2024
Curcuma
longae
Rhizoma,
commonly
known
as
turmeric,
is
extensively
utilized
not
only
in
Traditional
Chinese
Medicine
(TCM)
but
also
across
various
traditional
medicine
systems
worldwide.
It
renowned
for
its
effectiveness
removing
blood
stasis,
promoting
circulation,
and
relieving
pain.
The
primary
bioactive
metabolites
of
Rhizoma-curcumin,
β-elemene,
curcumol,
curdione-have
been
studied
their
pharmacological
benefits.
These
include
anti-tumor
properties,
cardiovascular
cerebrovascular
protection,
immune
regulation,
liver
roles
analgesics,
anti-inflammatories,
antivirals,
antibacterials,
hypoglycemics,
antioxidants.
This
review
critically
examines
the
extensive
body
research
regarding
mechanisms
action
which
engages
multiple
molecular
targets
signaling
pathways
such
NF-κB,
MAPKs,
PI3K/AKT.
core
objective
this
to
assess
how
main
active
turmeric
interact
with
these
achieve
therapeutic
outcomes
clinical
settings.
Furthermore,
we
discuss
challenges
related
bioavailability
explore
potential
methods
enhance
effects.
By
doing
so,
aims
provide
fresh
insights
into
optimization
Rhizoma
broader
applications.
Biomolecules,
Год журнала:
2024,
Номер
14(4), С. 387 - 387
Опубликована: Март 23, 2024
Curcumae
Rhizoma,
a
traditional
Chinese
medicine
with
wide
range
of
pharmacological
activities,
is
obtained
from
the
dried
rhizomes
Curcuma
phaeocaulis
VaL.,
kwangsiensis
S.
G.
Lee
et
C.
F.
Liang,
and
wenyujin
Y.
H.
Chen
Ling.
Sesquiterpenoids
curcuminoids
are
found
to
be
main
constituents
Rhizoma.
composed
three
isoprene
units
susceptible
complex
transformations,
such
as
cyclization,
rearrangement,
oxidation.
They
most
structurally
diverse
class
plant-based
natural
products
biological
activities
widely
in
nature.
In
recent
years,
scholars
have
conducted
abundant
studies
on
structures
properties
components
This
article
elucidates
chemical
structures,
medicinal
properties,
sesquiterpenoids
(a
total
274
compounds)
isolated
We
summarized
extraction
isolation
methods
for
sesquiterpenoids,
established
component
library
analyzed
structural
variances
among
sourced
Rhizoma
botanical
origins.
Furthermore,
our
investigation
reveals
array
sesquiterpenoid
types,
encompassing
guaiane-type,
germacrane-type,
eudesmane-type,
elemane-type,
cadinane-type,
carane-type,
bisabolane-type,
humulane-type,
other
emphasizing
relationship
between
diversity
activity.
hope
provide
valuable
reference
further
research
exploitation
pave
way
development
new
drugs
derived
plants.
Journal of Nanobiotechnology,
Год журнала:
2024,
Номер
22(1)
Опубликована: Окт. 8, 2024
Ferroptosis,
distinct
from
apoptosis,
necrosis,
and
autophagy,
is
a
unique
type
of
cell
death
driven
by
iron-dependent
phospholipid
peroxidation.
Since
ferroptosis
was
defined
in
2012,
it
has
received
widespread
attention
researchers
worldwide.
From
biochemical
perspective,
the
regulation
strongly
associated
with
cellular
metabolism,
primarily
including
iron
lipid
redox
metabolism.
The
distinctive
regulatory
mechanism
holds
great
potential
for
overcoming
drug
resistance-a
major
challenge
treating
cancer.
considerable
role
nanobiotechnology
disease
treatment
been
widely
reported,
but
further
more
systematic
discussion
on
how
enhances
therapeutic
efficacy
ferroptosis-associated
diseases
still
needs
to
be
improved.
Moreover,
while
exciting
cancer
relatively
well
summarized,
its
applications
other
diseases,
such
as
neurodegenerative
cardiovascular
cerebrovascular
kidney
disease,
remain
underreported.
Consequently,
necessary
fill
these
gaps
complete
ferroptosis.
In
this
review,
we
provide
an
extensive
introduction
background
elaborate
network.
Subsequently,
discuss
various
advantages
combining
enhance
reduce
side
effects
diseases.
Finally,
analyze
feasibility
improving
clinical
outcomes
based
needs,
current
limitations
future
directions
ferroptosis,
which
will
not
only
significant
guidance
also
accelerate
their
translations.
