Phytochemistry Reviews, Год журнала: 2024, Номер unknown
Опубликована: Дек. 20, 2024
Язык: Английский
Phytochemistry Reviews, Год журнала: 2024, Номер unknown
Опубликована: Дек. 20, 2024
Язык: Английский
Molecules, Год журнала: 2024, Номер 29(7), С. 1575 - 1575
Опубликована: Апрель 1, 2024
Objective: This study aimed to investigate methodologies for the extraction and purification of polysaccharides from Rosa roxburghii Tratt fruits their impact on various cellular processes in prostate cancer DU145 cells, including survival rate, migration, invasion, cell cycle, apoptosis. Results: Compared control group, polysaccharide exhibited a significant reduction viability, invasion rates cells time- dose-dependent manner within polysaccharide-treated groups. Additionally, it effectively arrested cycle at G0/G1 phase by downregulating expressions CDK-4, CDK-6, Cyclin D1. Furthermore, induced apoptosis upregulating Caspase 3, 8, 9, BAX. Methods: Polysaccharides were extracted sourced Yunnan, China. Extraction decolorization methods optimized using response surface methodology, based single-factor experiment. Polysaccharide was carried out DEAE-52 cellulose Sephadex G-100 column chromatography. The optimal dosage R. affecting determined CCK-8 assay. Cell migration assessed transwell scratch assays. Flow cytometry employed analyze effects Western blotting Quantitative real-time PCR utilized examine protein mRNA respectively. Conclusions: polysaccharides, consisting D-mannose, L-rhamnose, N-acetyl-D-glucosamine, D-galacturonic acid, D-glucose, D-galactcose, D-xylose, L-arabinose, L-fucose, possess ability hinder proliferation, while inducing through modulation relevant gene expressions.
Язык: Английский
Процитировано
2Current Cancer Drug Targets, Год журнала: 2024, Номер 24(7), С. 749 - 759
Опубликована: Фев. 4, 2024
The purpose of this research was to settle the role brucine in pancreatic ductal adenocarcinoma (PDAC) and mechanisms involved.
Язык: Английский
Процитировано
1Sustainable Chemistry and Pharmacy, Год журнала: 2024, Номер 42, С. 101837 - 101837
Опубликована: Ноя. 15, 2024
Язык: Английский
Процитировано
1Phytochemistry Reviews, Год журнала: 2024, Номер unknown
Опубликована: Дек. 20, 2024
Язык: Английский
Процитировано
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