Inhibition of Proliferation and Induction of Apoptosis in Prostatic Carcinoma DU145 Cells by Polysaccharides from Yunnan Rosa roxburghii Tratt

Molecules, Год журнала: 2024, Номер 29(7), С. 1575 - 1575

Опубликована: Апрель 1, 2024

Objective: This study aimed to investigate methodologies for the extraction and purification of polysaccharides from Rosa roxburghii Tratt fruits their impact on various cellular processes in prostate cancer DU145 cells, including survival rate, migration, invasion, cell cycle, apoptosis. Results: Compared control group, polysaccharide exhibited a significant reduction viability, invasion rates cells time- dose-dependent manner within polysaccharide-treated groups. Additionally, it effectively arrested cycle at G0/G1 phase by downregulating expressions CDK-4, CDK-6, Cyclin D1. Furthermore, induced apoptosis upregulating Caspase 3, 8, 9, BAX. Methods: Polysaccharides were extracted sourced Yunnan, China. Extraction decolorization methods optimized using response surface methodology, based single-factor experiment. Polysaccharide was carried out DEAE-52 cellulose Sephadex G-100 column chromatography. The optimal dosage R. affecting determined CCK-8 assay. Cell migration assessed transwell scratch assays. Flow cytometry employed analyze effects Western blotting Quantitative real-time PCR utilized examine protein mRNA respectively. Conclusions: polysaccharides, consisting D-mannose, L-rhamnose, N-acetyl-D-glucosamine, D-galacturonic acid, D-glucose, D-galactcose, D-xylose, L-arabinose, L-fucose, possess ability hinder proliferation, while inducing through modulation relevant gene expressions.

Язык: Английский

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Brucine Inhibits Proliferation of Pancreatic Ductal Adenocarcinoma through PI3K/AKT Pathway-induced Mitochondrial Apoptosis

Current Cancer Drug Targets, Год журнала: 2024, Номер 24(7), С. 749 - 759

Опубликована: Фев. 4, 2024

The purpose of this research was to settle the role brucine in pancreatic ductal adenocarcinoma (PDAC) and mechanisms involved.

Язык: Английский

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Design and green synthesis of asymmetric 3,4,5-trimethoxyphenyl-inspired 1,5-diaryl-1,4-pentadien-3-ones as potent anti-gastric cancer agents through ROS and JNK-triggered apoptosis

Sustainable Chemistry and Pharmacy, Год журнала: 2024, Номер 42, С. 101837 - 101837

Опубликована: Ноя. 15, 2024

Язык: Английский

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Pentacyclic triterpenoids: diversity, distribution and their propitious pharmacological potential

Phytochemistry Reviews, Год журнала: 2024, Номер unknown

Опубликована: Дек. 20, 2024

Язык: Английский

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