International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(22), С. 12463 - 12463
Опубликована: Ноя. 20, 2024
Skeletal
muscle
atrophy
is
a
major
health
concern,
severely
affecting
the
patient's
mobility
and
life
quality.
In
pathological
process
of
skeletal
atrophy,
with
progressive
decline
in
quality,
strength,
function,
incidence
falling,
fracture,
death
greatly
increased.
Unfortunately,
there
are
no
effective
treatments
for
this
devastating
disease.
Thus,
it
imperative
to
investigate
exact
molecular
mechanisms
underlying
development
identify
new
therapeutic
targets.
Decreased
mass,
fiber
cross-sectional
area
typical
features
manifestations
atrophy.
Ferroptosis,
an
emerging
type
programmed
cell
death,
characterized
by
iron-dependent
oxidative
damage,
lipid
peroxidation,
reactive
oxygen
species
accumulation.
Notably,
understanding
its
role
emerging.
Ferroptosis
has
been
found
play
important
intricate
interplay
between
progression
caused
multiple
factors.
This
provides
opportunities
challenges
treatment
Therefore,
we
systematically
elucidated
ferroptosis
mechanism
progress
aiming
provide
comprehensive
insight
into
relationship
from
perspectives
iron
metabolism
peroxidation
insights
targeting
pathways
related
International Journal of Biological Sciences,
Год журнала:
2024,
Номер
20(9), С. 3621 - 3637
Опубликована: Янв. 1, 2024
Ferroptosis,
an
emerging
type
of
programmed
cell
death,
is
initiated
by
iron-dependent
and
excessive
ROS-mediated
lipid
peroxidation,
which
eventually
leads
to
plasma
membrane
rupture
death.
Many
canonical
signalling
pathways
biological
processes
are
involved
in
ferroptosis.
Furthermore,
cancer
cells
more
susceptible
ferroptosis
due
the
high
load
ROS
unique
metabolic
characteristics,
including
iron
requirements.
Recent
investigations
have
revealed
that
plays
a
crucial
role
progression
tumours,
especially
HCC.
Specifically,
induction
can
not
only
inhibit
growth
hepatoma
cells,
thereby
reversing
tumorigenesis,
but
also
improves
efficacy
immunotherapy
enhances
antitumour
immune
response.
Therefore,
triggering
has
become
new
therapeutic
strategy
for
therapy.
In
this
review,
we
summarize
characteristics
based
on
its
underlying
mechanism
HCC
provide
possible
applications.
Lung
ischemia–reperfusion
(I/R)
injury
is
a
common
clinical
pathology
associated
with
high
mortality.
The
pathophysiology
of
lung
I/R
involves
ferroptosis
and
elevated
protein
O-GlcNAcylation
levels,
while
the
effect
on
remains
unclear.
This
research
aimed
to
explore
reducing
in
pulmonary
epithelial
cells
caused
by
I/R.
First,
we
identified
O-GlcNAc
transferase
1
(Ogt1)
as
differentially
expressed
gene
acute
injury/acute
respiratory
distress
syndrome
(ALI/ARDS)
patients,
using
single-cell
sequencing,
Gene
Ontology
analysis
(GO
analysis)
revealed
enrichment
process.
We
found
time-dependent
dynamic
alteration
during
injury.
Proteomics
proteins
enriched
multiple
redox-related
pathways
based
KEGG
annotation.
Thus,
generated
Ogt1-conditional
knockout
mice
that
Ogt1
deficiency
aggravated
ferroptosis,
evidenced
lipid
reactive
oxygen
species
(lipid
ROS),
malondialdehyde
(MDA),
Fe2+,
well
alterations
critical
expression
glutathione
peroxidase
4
(GPX4)
solute
carrier
family
7
member
11
(SLC7A11).
Consistently,
inhibited
sensitivity
hypoxia/reoxygenation
(H/R)
injury-induced
TC-1
via
O-GlcNAcylated
NF-E2-related
factor-2
(Nrf2).
Furthermore,
both
chromatin
immunoprecipitation
(ChIP)
assay
dual-luciferase
reporter
indicated
Nrf2
could
bind
translation
start
site
(TSS)
glucose-6-phosphate
dehydrogenase
(G6PDH)
promote
its
transcriptional
activity.
As
an
important
rate-limiting
enzyme
pentose
phosphate
pathway
(PPP),
G6PDH
provided
mass
nicotinamide
adenine
dinucleotide
(NADPH)
improve
redox
state
(GSH)
eventually
led
resistance.
Rescue
experiments
proved
knockdown
or
Nrf2-T334A
(O-GlcNAcylation
site)
mutation
abolished
protective
In
summary,
protect
against
Nrf2/G6PDH
pathway.
Our
work
will
provide
new
basis
for
therapeutic
strategies
ischemia–reperfusion-induced
Aryl
hydrocarbon
receptor
(AHR)
is
a
ligand-activated
transcription
factor.
In
lung
cancer,
AHR
activation
stimulates
cancer
cell
proliferation
and
promotes
tissue
invasion
metastasis,
targeting
the
pathway
an
effective
way
to
prevent
treat
cancer.
binds
NRF2
promoter
region
promote
carcinogenesis,
but
treatment
research
based
on
AHR/NRF2
insufficient.
3,3′-diindolylmethane
(DIM),
active
phytochemical
derivative
extracted
from
cruciferous
vegetables,
modulator
of
AHR.
this
study,
We
investigated
medicinal
value
DIM
in
NSCLC
(non-small
cancer)
by
vivo
vitro
experiments
explored
underlying
mechanisms.
studies
showed
that
inhibited
viability
induced
apoptosis
cycle
arrest
cells.
migration
cells
reversing
epithelial-mesenchymal
transition.
ferroptosis
cells;
increased
cellular
Fe2+,
ROS
(reactive
oxygen
species),
MDA;
decreased
GSH,
AHR,
NRF2,
GPX4;
disrupted
mitochondrial
membrane
potential.
The
effect
DIM-induced
can
be
reversed
antagonist
CH-223191,
inhibitor
Fer-1,
scavenger
NAC.
Overexpression
ferroptosis.
Identical
results
were
obtained
nude
mouse
xenograft
model.
summary,
we
have
confirmed
has
significant
potential
non-small
induces
through
AHR/NRF2/GPX4
axis.
These
findings
provide
experimental
basis
for
future
clinical
Separation Science Plus,
Год журнала:
2025,
Номер
8(3)
Опубликована: Март 1, 2025
ABSTRACT
Paris
polyphylla
var.
yunnanensis
(PPY)
is
a
traditional
and
valuable
Chinese
medicine
in
China,
which
one
of
the
important
raw
materials
for
many
proprietary
medicines,
but
its
wild
resources
are
scarce
quality
cultivated
products
varies.
Steroidal
saponins
main
active
components
PPY;
order
to
analyze
differences
chemical
constituents
contained
different
parts
PPY,
present
study
was
conducted
characterize
steroidal
PPY
(axial
roots,
stems,
leaves)
by
ultrahigh‐performance
liquid
chromatography‐quadrupole
time‐of‐flight
tandem
mass
spectrometry
(UHPLC‐Q/TOF‐HRMS)
coupled
with
molecular
network
technology
determine
contents
nine
(tall
short
stalk)
high‐performance
chromatography
photodiode
array
(HPLC‐PDA).
The
results
showed
that
62
were
identified
from
there
significant
composition
types
parts,
among
axial
roots
leaves
higher,
while
those
stems
lower.
three
specified
China
Pharmacopoeia
(2020
edition)
higher
than
0.6%
leaves,
indicating
can
be
used
as
potential
sources
saponins.
similar
tall
stalks,
total
saponin
content
taller
stalk.
In
conclusion,
this
points
out
direction
resource
conservation
rational
development
provides
basis
comprehensive
evaluation
medicinal
herbs
screening
high‐quality
germplasm.
