Geographical variation in metabolite profiles and bioactivity of Thesium chinense Turcz. revealed by UPLC-Q-TOF-MS-based metabolomics
Frontiers in Plant Science,
Год журнала:
2025,
Номер
15
Опубликована: Янв. 10, 2025
This
study
aims
to
investigate
the
impact
of
geographical
origin
on
metabolite
composition
and
bioactivity
Thesium
chinense
Turcz.
(TCT),
a
member
Apiaceae
family
renowned
for
its
wide
range
pharmacological
properties,
including
antioxidant,
antimicrobial,
anti-inflammatory
effects.
In
this
study,
we
investigated
whole
plants
TCT
from
different
regions
in
China,
aiming
explore
variation
TCT.
A
non-targeted
metabolomics
approach
was
employed
using
ultra-high-performance
liquid
chromatography
combined
with
quadrupole
time-of-flight
mass
spectrometry
(UPLC-Q-TOF-MS).
Principal
component
analysis
(PCA)
partial
least
squares
discriminant
(PLS-DA)
were
utilized
identify
differentiate
profiles.
We
bioactivity,
antioxidant
activity,
total
flavonoid
content
(TFC),
characteristic
compounds
sourced
regions.
further
metabolic
differences
quality
characteristics
various
origins.
PCA
PLS-DA
analyses
indicated
that
samples
origins
could
be
clearly
distinguished.
The
revealed
54
differential
metabolites,
predominantly
flavonoids
alkaloids.
KEGG
pathway
significant
variations
biosynthesis
pathways
flavanols
among
samples.
Anhui
province
exhibited
highest
TFC
strongest
activities,
while
Jilin
showed
lowest.
strong
correlation
observed
between
origins,
suggesting
is
significantly
influenced
by
provenance.
Additionally,
activities
validated,
showing
predictive
relationship
TFC.
research
highlights
potential
discerning
subtleties
plant
metabolomes,
contributing
advancement
traditional
Chinese
medicine
integration
into
modern
healthcare
practices.
Язык: Английский
Chemical characterization of Siraitia grosvenorii granules and their efficacy and mechanism of action on PM2.5-induced acute lung injury
Ecotoxicology and Environmental Safety,
Год журнала:
2025,
Номер
290, С. 117702 - 117702
Опубликована: Янв. 1, 2025
Язык: Английский
[
PubMed,
Год журнала:
2025,
Номер
45(2), С. 285 - 295
Опубликована: Фев. 20, 2025
To
investigate
the
therapeutic
mechanism
of
Thesium
chinense
Turcz.
(TCT)
for
antibiotic-associated
diarrhea
(AAD).
Network
pharmacology,
KEGG
pathway
enrichment
analysis
and
molecular
docking
were
used
to
identify
shared
targets
genes
TCT
AAD,
key
signaling
pathways
binding
between
active
components
in
core
protein
targets.
In
a
Kunming
mouse
model
AAD
established
by
intragastric
administration
lincomycin
hydrochloride,
effects
daily
gavage
1%
carboxymethyl
cellulose
sodium
or
gel
solutions
at
1.5
g/kg
3
(n=10)
on
body
weight
observed.
HE
staining,
ELISA,
16S
rRNA
sequencing,
Western
blotting
examine
pathologies,
expression
levels
IL-6
TNF-α,
changes
gut
microbiota,
expressions
EGFR,
p-EGFR,
PI3K,
p-PI3K,
Akt,
p-Akt
colon
tissues
mice.
We
identified
total
66
68
including
STAT3
PIK3CA.
suggested
that
was
mediated
primarily
through
PI3K/Akt
pathway.
Molecular
showed
EGFR
had
highest
affinity
with
coniferin,
EGFR-coniferin
complex
maintained
stable
conformation
10
ns,
whose
stability
also
confirmed
Gibbs
free
energy
analysis.
models
treatment
significantly
improved
colonic
tissue
morphology,
decreased
TNF-α
IL-6,
increased
microbiota
diversity,
modulated
relative
abundances
genera
Lactobacillus
Bacteroides.
markedly
reduced
p-PI3K
can
alleviate
mice
modulating
composition,
regulating
EGFR/PI3K/Akt
pathway,
reducing
TNF‑α
expressions.
Язык: Английский
The integration of metabolites from Forsythia suspensa and gut microbiota ameliorates drug-induced liver injury: network pharmacology and molecular docking studies
Artificial Cells Nanomedicine and Biotechnology,
Год журнала:
2025,
Номер
53(1), С. 105 - 121
Опубликована: Март 7, 2025
This
study
integrates
metabolites
from
Forsythia
suspensa
(FS)
and
gut
microbiota
GM
to
assess
combined
therapeutic
efficacy
against
drug-induced
liver
injury
(DILI)
using
network
pharmacology
molecular
docking.
Metabolites
of
FS
were
retrieved
the
NPASS
gutMGene
databases,
respectively.
Relevant
targets
for
DILI-related
identified
through
public
databases.
The
PPI
KEGG
pathway
analysis
employed
identify
hub
key
signalling
pathways.
Furthermore,
we
performed
a
docking
assay
on
active
verify
pharmacological
concept.
physicochemical
properties
toxicity
assessed
in
silico
platforms.
19
final
recognized
as
proteins
responsible
alleviation
DILI
by
metabolites,
with
ESR1
emerging
central
target
network.
estrogen
pathway,
particularly
involving
ESR1,
ESR2
JUN
genes,
was
mediator
effects.
Four
(baicalein,
luteolin,
lunularin
2,3-bis(3,4-dihydroxybenzyl)butyrolactone)
two
(pinoresinol
isolariciresinol)
non-toxic,
promising
candidates.
In
conclusion,
may
exert
potent
synergistic
effect
modulation
pathway.
Язык: Английский
Fabrication of sodium alginate/polyvinyl alcohol@ ZnNPs with SPI scaffold for evaluation of immune-stimulating and liver-protective effects in methotrexate-treated rats
International Journal of Biological Macromolecules,
Год журнала:
2025,
Номер
unknown, С. 143514 - 143514
Опубликована: Апрель 1, 2025
Язык: Английский
Potential Hepatoprotective Effects of Chamaecyparis lawsoniana against Methotrexate-Induced Liver Injury: Integrated Phytochemical Profiling, Target Network Analysis, and Experimental Validation
Antioxidants,
Год журнала:
2023,
Номер
12(12), С. 2118 - 2118
Опубликована: Дек. 14, 2023
Methotrexate
(MTX)
therapy
encounters
significant
limitations
due
to
the
concern
of
drug-induced
liver
injury
(DILI),
which
poses
a
challenge
its
usage.
To
mitigate
deleterious
effects
MTX
on
hepatic
function,
researchers
have
explored
plant
sources
discover
potential
hepatoprotective
agents.
This
study
investigated
ethanolic
extract
derived
from
aerial
parts
Chamaecyparis
lawsoniana
(CLAE)
against
DILI,
specifically
focusing
MTX-induced
hepatotoxicity.
UPLC-ESI-MS/MS
was
used
identify
61
compounds
in
CLAE,
with
31
bioactive
determined
through
pharmacokinetic
analysis.
Network
pharmacology
analysis
revealed
195
DILI
targets
for
compounds,
including
TP53,
IL6,
TNF,
HSP90AA1,
EGFR,
IL1B,
BCL2,
and
CASP3
as
top
targets.
In
vivo
experiments
conducted
rats
acute
MTX-hepatotoxicity
that
administering
CLAE
orally
at
200
400
mg/kg/day
ten
days
dose-dependently
improved
attenuated
oxidative
stress,
inflammation,
apoptosis,
reversed
disarrayed
histological
features
induced
by
MTX.
general,
findings
present
provide
evidence
favor
capabilities
thereby
justifying
need
additional
preclinical
clinical
investigations.
Язык: Английский
Monotropein mitigates methotrexate-induced liver injury by activating autophagy and inhibiting ferroptosis
Journal of Functional Foods,
Год журнала:
2024,
Номер
121, С. 106413 - 106413
Опубликована: Авг. 18, 2024
Methotrexate
(MTX)
is
effective
for
the
therapy
of
cancer,
lupus
erythematosus,
rheumatoid
arthritis,
psoriasis
and
other
diseases.
However,
hepatotoxicity
MTX
often
leads
to
withdrawal
discontinuation
drugs,
then
limits
its
clinical
application.
Monotropein
(MON),
an
iridoid
glycosides
distributing
in
many
edible
medicinal
plants,
has
been
exhibited
prevent
liver
kedney
injury
induced
by
chemicals
related
The
current
study
aimed
assess
potential
beneficial
effects
MON
mitigating
MTX-induced
together
with
delineating
implicated
mechanisms.
A
model
mice
BRL
3A
cells
were
developed
explore
hepatoprotection
MON.
GEO
WGCNA
analysis
conducted
predict
possible
mechanism
on
injury.
results
demonstrated
that
can
decrease
serum
levels
AST
ALT,
increase
activities
antioxidant
SOD
GSH,
improve
morphological
alteration
tissue,
reduce
accumulation
lipids
hepatocytes
tissue
treated
MTX.
Moreover,
our
also
activate
autophagy
as
assessed
autophagosome
formation,
expression
protein
autophagy,
inhibit
ferroptosis
evaluated
mitochondrial
integrity,
Fe2+level
key
changes,
CQ
(inhibitor
autophagy)
RSL-3
(the
activator
ferroptosis)
reversed
injured
Therefore,
attenuates
activating
inhibiting
ferroptosis.
These
findings
provide
new
insights
into
a
healthy
ingredient
functional
food.
Язык: Английский