Oligoasthenozoospermia is alleviated in a mouse model by [Gly14]‐humanin‐mediated attenuation of oxidative stress and ferroptosis DOI Open Access
Yumeng Liu, Liping Zou, Liping Zou

и другие.

Andrology, Год журнала: 2024, Номер unknown

Опубликована: Окт. 22, 2024

Abstract Background Oligoasthenozoospermia is a common cause of male infertility, for which effective treatments are urgently needed. Humanin (HN) peptide associated with this condition. Objectives To investigate the ameliorative effect [Gly14]‐Humanin (HNG) on oligoasthenozoospermia and mechanisms. Materials methods Mice were treated cyclophosphamide (CP) to construct mice model oligoasthenozoospermia. The resulting saline or HNG. Subsequently, testis weights, organ indices, testicular structure, sperm counts motilities, litter sizes, serum testosterone concentrations determined. Differential gene expression in tissues was determined by RNA sequencing. TM3, TM4, GC1, GC2 cells exposed erastin induce ferroptosis, followed treatment HNG + ML385 (a nuclear factor erythroid 2‐related 2 inhibitor). Levels reactive oxygen species (ROS), malondialdehyde (MDA), glutathione (GSH), ferrous ions (Fe 2+ ) their ferroptosis‐related proteins immunofluorescence western blot. Results improved parameters increased size mice. Kyoto Encyclopaedia Genes Genomes pathway enrichment analysis revealed significant differential genes. after treatment. ROS, MDA, Fe decreased GSH TM3 TM4 In vitro experiments confirmed that activated 2/glutathione peroxidase 4 (Nrf2/GPX4) pathway. However, these effects blocked Discussion conclusion demonstrated therapeutic mouse reducing oxidative stress ferroptosis. cells, attenuated cellular inhibited ferroptosis via Nrf2/GPX4

Язык: Английский

Ferroptosis in senescence and age-related diseases: pathogenic mechanisms and potential intervention targets DOI
Chang Liu, Jie Pan,

Qi Bao

и другие.

Molecular Biology Reports, Год журнала: 2025, Номер 52(1)

Опубликована: Фев. 17, 2025

Язык: Английский

Процитировано

1
Delay the progression of glucocorticoid‐induced osteoporosis: Fraxin targets ferroptosis via the Nrf2/GPX4 pathway DOI
Xiang Zheng,

Fang‐Chen Ye,

Tao Sun

и другие.

Phytotherapy Research, Год журнала: 2024, Номер 38(11), С. 5203 - 5224

Опубликована: Авг. 27, 2024

Abstract Glucocorticoid‐induced osteoporosis (GIOP) commonly accelerates bone loss, increasing the risk of fractures and osteonecrosis more significantly than traditional menopausal osteoporosis. The extracellular environment influenced by glucocorticoids heightens fracture risks. Fraxin (Fra), a key component Chinese herbal remedy Cortex Fraxini , is known for its wide‐ranging pharmacological effects, but impact on GIOP remains unexplored. This investigation aims to delineate effects underlying mechanisms Fra in combating dexamethasone (Dex)‐induced ferroptosis GIOP. We established mouse model via intraperitoneal injections Dex cultured osteoblasts with treatment vitro analysis. evaluated Dex‐treated through assays such as C11‐BODIPY FerroOrange staining, mitochondrial functionality tests, protein expression analyses Western blot immunofluorescence. influence microarchitecture mice was assessed using microcomputerized tomography, hematoxylin eosin double‐labeling Calcein–Alizarin Red S, immunohistochemistry at imaging histological levels. Based our data, prevented Dex‐induced loss. In vitro, glutathione levels increased malondialdehyde, lipid peroxidation, reactive oxygen species decreased. also increases nuclear factor erythroid 2‐related 2 (Nrf2), peroxidase 4 (GPX4), COL1A1 promotes formation. To delve deeper into mechanism, findings revealed that triggered activation Nrf2/GPX4 signaling. Moreover, use siRNA‐Nrf2 blocked beneficial effect cultivated Dex. effectively combats activating signaling pathway inhibit ferroptosis.

Язык: Английский

Процитировано

4
LuQi Formula Ameliorates Pressure Overload-induced Heart Failure by Regulating Macrophages and Regulatory T cells DOI
Xinting Wang, Qian Liu, Peipei Cheng

и другие.

Phytomedicine, Год журнала: 2025, Номер unknown, С. 156527 - 156527

Опубликована: Фев. 1, 2025

Язык: Английский

Процитировано

0
Morroniside alleviates cisplatin-induced renal injury and gut dysbiosis via the gut–kidney axis and ferroptosis DOI
H Li, Ke Xu,

Wenhao Mao

и другие.

International Immunopharmacology, Год журнала: 2025, Номер 153, С. 114430 - 114430

Опубликована: Март 17, 2025

Язык: Английский

Процитировано

0
Single-cell multi-omics analysis reveals the mechanism of action of a novel antioxidant polyphenol nanoparticle loaded with STAT3 agonist in mediating cardiomyocyte ferroptosis to ameliorate age-related heart failure DOI Creative Commons
Haoyuan Zheng, Yuan Tian, Dongyu Li

и другие.

Journal of Nanobiotechnology, Год журнала: 2025, Номер 23(1)

Опубликована: Март 29, 2025

Heart failure (HF) is a prevalent and critical cardiac condition that leads to profound structural functional changes in the heart. Although traditional treatments have shown partial efficacy, long-term outcomes remain suboptimal. Emerging research has highlighted pivotal role of oxidative stress ferroptosis HF progression. This study investigates new therapeutic approach utilizing antioxidant polyphenol nanoparticles loaded with STAT3 agonist (PN@Col) target these pathways improve age-related HF. Key cells genes contributing progression were identified via analysis GEO database, single-cell RNA sequencing (scRNA-seq) AUCell used evaluate differential gene expression. The was as essential, its functionality further validated vitro through cell experiments, confirming impact on cardiomyocytes (CMs) Following development PN@Col, experiments showed PN@Col effectively reduced CMs. In vivo studies elderly mice demonstrated significant improvements function following treatment. offers promising by mitigating cardiomyocytes. These findings provide solid scientific foundation for potential novel treatment strategy HF, supporting exploration toward clinical application.

Язык: Английский

Процитировано

0
Mitochondrial function: A new direction for the targeted treatment of cardiovascular diseases with Chinese herbal medicine DOI Creative Commons
Lin Yang, Liang Wang, Baofeng Yang

и другие.

Journal of Holistic Integrative Pharmacy, Год журнала: 2025, Номер 6(1), С. 91 - 104

Опубликована: Март 1, 2025

Язык: Английский

Процитировано

0
Natural products and ferroptosis: A novel approach for heart failure management DOI
Zeyu Zhang, Zhihua Yang, Shuai Wang

и другие.

Phytomedicine, Год журнала: 2025, Номер 142, С. 156783 - 156783

Опубликована: Апрель 18, 2025

Язык: Английский

Процитировано

0
Rituximab-Chidamide combination chemotherapy enhances autophagy to overcome drug resistance in diffuse large B-cell lymphoma DOI

Zelai Wu,

Dongni Wang, Di Fu

и другие.

International Immunopharmacology, Год журнала: 2025, Номер 156, С. 114578 - 114578

Опубликована: Апрель 20, 2025

Язык: Английский

Процитировано

0
Nrf2 mediated signaling axis in heart failure: Potential pharmacological receptor DOI Creative Commons
Peipei Cheng, Xinting Wang, Qian Liu

и другие.

Pharmacological Research, Год журнала: 2024, Номер 206, С. 107268 - 107268

Опубликована: Июнь 20, 2024

Heart failure (HF) has emerged as the most pressing health concerns globally, and extant clinical therapies are accompanied by side effects patients have a high burden of financial. The protein products nuclear factor erythroid 2-related 2 (Nrf2) target genes variety cardioprotective effects, including antioxidant, metabolic functions anti-inflammatory. By evaluating established preclinical research in HF to date, we explored potential Nrf2 exert unique novel therapeutic receptor for HF. In this review, generalize progression, structure, function cardiovascular system. mechanism action involved well agonists natural compounds summarized. Additionally, discuss challenges implications future translation application pharmacology targeting Nrf2. It's critical developing new drugs

Язык: Английский

Процитировано

2
Multi-Omics Exploration of the Mechanism of Curcumol to Reduce Invasion and Metastasis of Nasopharyngeal Carcinoma by Inhibiting NCL/EBNA1-Mediated UBE2C Upregulation DOI Creative Commons
Haiping Liu, Juan Wang, Lin Wang

и другие.

Biomolecules, Год журнала: 2024, Номер 14(9), С. 1142 - 1142

Опубликована: Сен. 9, 2024

Nasopharyngeal carcinoma (NPC) is closely linked to Epstein–Barr virus (EBV) infection. Curcumae Rhizoma, a traditional Chinese herb, has shown antitumor effects, primarily through its component curcumol (Cur), which been reduce NPC cell invasion and migration by targeting nucleolin (NCL) Virus Nuclear Antigen 1 (EBNA1). We constructed an EBV-positive model using C666-1 cells performed transcriptomics studies after treatment with curcumol, revealed significant enrichment of ubiquitin-mediated proteolysis, the PI3K-AKT mTOR signaling pathways, cycle apoptosis involved in tumor migration. To investigate importance NCL EBNA1 curcumol-resistant NPC, we multi-omics study short hairpin (shNCL) shEBNA1 cells, proteomics results showed complement coagulation cascades proteolysis pathways. Here, focused on ubiquitin-conjugating enzyme E2C (UBE2C), plays important role pathway. In addition, metabolomics that UBE2C highly associated 4-Aminobutanoic acid (GABA). vitro further validated function key targets, suggesting EBNA1-mediated resistance nasopharyngeal metastasis.

Язык: Английский

Процитировано

0