BuyangHuanwu Decoction alleviates Endothelial Cell Apoptosis and Coronary Microvascular Dysfunction via Regulation of the MAPKK4/p38 Signaling Axis

International Journal of Medical Sciences, Год журнала: 2024, Номер 21(13), С. 2464 - 2479

Опубликована: Янв. 1, 2024

MAPKK4 has been implicated in the pathological mechanisms underlying myocardial and vascular injury, specifically influencing endothelial cell damage programmed death via subcellular pathways. Nevertheless, regulatory role of coronary microvascular injury following infarction remains unconfirmed, exploration targeted mitochondrial protective therapeutic agents unaddressed. In light this gap, we established a gene-modified mouse model ischemia-reperfusion employed Buyang Huanwu decoction (BYHW), traditional cardiovascular formula, to assess its efficacy treating post-ischemia-reperfusion. The study aimed elucidate mechanism by which BYHW mitigates induced through attenuation apoptosis. Experimental outcomes revealed that high-dose significantly ameliorated post-ischemia-reperfusion, restoring structural integrity microvasculature reducing inflammation oxidative stress. Contrarily, transgenic mice overexpressing MAPKK4, intervention failed attenuate To further investigate, simulated hypoxia/reoxygenation cells using MAPKK4-related cellular gene modification model. results indicated attenuates inflammatory enhances viability hypoxic stress, inhibiting apoptosis pathway. However, overexpression MAPKK4/p38 negated effects BYHW, showing no impact on stress under conditions. Molecular interaction studies confirmed active components Astragaloside IV Ligustrazine, interact with MAPKK4/P38 axis.

Язык: Английский

---

The Bidirectional Role of Hypoxia-Inducible Factor 1 Alpha in Vascular Dementia Caused by Chronic Cerebral Hypoperfusion

Molecular Neurobiology, Год журнала: 2025, Номер unknown

Опубликована: Апрель 9, 2025

Язык: Английский

---

CAVIN3 deficiency promotes vascular normalization in ocular neovascular disease via ERK/JAG1 signaling pathway

JCI Insight, Год журнала: 2025, Номер 10(9)

Опубликована: Май 7, 2025

Multiple members of the caveolae-associated protein (Cavin) family are implicated in angiogenesis. However, specific role CAVIN3 pathological angiogenesis within eye remains unclear. The present study demonstrated that knockdown endothelial cells (ECs) promoted vascular normalization ocular neovascularization. Elevated expression was observed ECs retinal pigment epithelium/choroid complexes from patients with neovascular age-related macular degeneration and fibrovascular membranes proliferative diabetic retinopathy. Additionally, upregulated Cavin3 detected laser-induced choroidal neovascularization (CNV) oxygen-induced retinopathy (OIR) mouse models. In both OIR CNV mice, inhibited deficiency further disrupted EC proliferation sprouting, thereby promoting by partially restoring microenvironmental hypoxia reestablishing pericyte-EC interactions. Mechanistically, we zinc finger E-box-binding homeobox 1 (ZEB1) regulated transcription under hypoxic conditions. modulated vascularization inhibiting ERK phosphorylation, which downregulated jagged (JAG1) expression. Conclusively, this elucidated protective models, addressing a gap understanding regulatory Cavins These findings suggested therapeutic direction for diseases.

Язык: Английский

---

Single-Cell Analysis Reveals the Cellular and Molecular Changes of Liver Injury and Fibrosis in Mice During the Progression of Schistosoma japonicum Infection

Current Issues in Molecular Biology, Год журнала: 2024, Номер 46(11), С. 11906 - 11926

Опубликована: Окт. 23, 2024

Schistosomiasis is a parasitic disease that poses serious threat to human health. However, the pathogenic mechanism during progression of

Язык: Английский

---

Mechanism of Buyang Huanwu Decoction mediating Cav1-regulated Wnt pathway to promote neural regeneration in cerebral ischemic mice

Journal of Ethnopharmacology, Год журнала: 2024, Номер 339, С. 119121 - 119121

Опубликована: Ноя. 17, 2024

Язык: Английский

---