SQYC formula improves the efficacy of PD-1 monoclonal antibodies in MSS colorectal cancer by regulating dendritic cell mitophagy via the PINK1-Parkin pathway
Phytomedicine,
Год журнала:
2025,
Номер
138, С. 156388 - 156388
Опубликована: Янв. 11, 2025
Язык: Английский
Exploring the Underlying Mechanism of Weiling Decoction Alleviates Cold-Dampness Diarrhea Based on Network Pharmacology, Transcriptomics, Molecular Docking and Experimental Validation
Pharmaceuticals,
Год журнала:
2025,
Номер
18(1), С. 109 - 109
Опубликована: Янв. 16, 2025
Background:
Cold-dampness
diarrhea
(CDD)
is
a
common
gastrointestinal
disorder
in
children,
characterized
by
and
intestinal
barrier
dysfunction.
Weiling
decoction
(WLD)
frequently
used
clinical
practice
to
treat
CDD,
condition
triggered
multiple
factors.
However,
the
molecular
mechanisms
underlying
its
therapeutic
effects
remain
poorly
understood.
Objectives:
This
study
aimed
evaluate
efficacy
of
WLD
treating
CDD
elucidate
potential
mechanisms.
Methods:
UPLC-HRMS/MS
was
employed
identify
chemical
constituents
absorption
components
plasma
WLD-treated
rats.
Additionally,
rat
model
established
assess
through
comprehensive
approach.
To
these
effects,
network
pharmacology
transcriptomic
analyses
were
performed
signaling
pathways
associated
with
alleviation.
Molecular
docking
flow
cytometry
assays
subsequently
utilized
validate
identified
pathways.
Results:
A
total
223
detected
WLD,
49
rats
UPLC-HRMS/MS.
treatment
significantly
alleviated
symptoms
reduced
damage,
diminished
inflammatory
response.
influenced
key
genes
immune-related
revealed
strong
binding
affinities
between
main
targets
within
Flow
cytometry,
along
analysis
cytokines
transcription
factors,
demonstrated
that
modulated
balance
Th1/Th2
Th17/Treg
cell
populations.
Conclusions:
provides
first
evidence
alleviates
regulating
These
findings
offer
theoretical
basis
for
future
investigations
into
CDD.
Язык: Английский
Deep Learning-based Multi-Brain Capsule network for Next-Gen Clinical Emotion Recognition using EEG Signals
Neuroscience Informatics,
Год журнала:
2025,
Номер
5(2), С. 100203 - 100203
Опубликована: Апрель 28, 2025
Язык: Английский
Decursin Suppresses Esophageal Squamous Cell Carcinoma Progression via Orchestrated Cell Cycle Deceleration, Apoptotic Activation, and Oncoprotein Degradation
International Journal of Molecular Sciences,
Год журнала:
2025,
Номер
26(11), С. 5391 - 5391
Опубликована: Июнь 4, 2025
Esophageal
squamous
cell
carcinoma
(ESCC)
remains
a
lethal
malignancy
with
limited
therapeutic
options.
This
study
investigated
the
antitumor
efficacy
and
mechanisms
of
decursin,
natural
pyranocoumarin
derivative,
against
ESCC.
In
vitro
analyses
demonstrated
that
decursin
selectively
inhibited
ESCC
viability
(IC50:
14.62
±
0.61–26.20
2.11
μM
across
TE-1,
KYSE-30,
KYSE-150
lines)
without
affecting
normal
esophageal
epithelial
cells
(Het-1A).
Decursin
(10
μM)
suppressed
colony
formation,
impaired
wound
healing
(p
<
0.001
at
48
h),
reduced
Transwell
migration/invasion
in
cells.
Subcutaneous
xenograft
models
revealed
significant
tumor
growth
inhibition
0.01)
treatment
mg/kg,
intraperitoneal),
accompanied
by
no
systemic
toxicity.
Mechanistically,
induced
G0/G1
cycle
deceleration
apoptosis
through
ubiquitin–proteasome-mediated
degradation
oncoproteins
TP63
SOX2.
Time-
dose-dependent
protein
suppression
was
reversed
proteasome
inhibitor
MG-132,
but
unaffected
lysosomal
inhibition.
These
findings
establish
as
promising
agent
for
ESCC,
functioning
via
proteasomal
key
oncogenic
drivers,
provide
rationale
decursin’s
further
development
targeted
monotherapy
or
chemosensitizer
multimodal
regimens.
Язык: Английский