Hydroxytyrosol Alleviates Acute Liver Injury by Inhibiting the TNF-α/PI3K/AKT Signaling Pathway via Targeting TNF-α Signaling DOI Open Access

Zhining Gao,

Hui Dai, Qinqin Zhang

и другие.

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(23), С. 12844 - 12844

Опубликована: Ноя. 29, 2024

Acute liver injury (ALI) is an to tissue caused by viruses, drugs, alcohol, and oxygen deprivation, one of the most common serious clinical disorders. Hydroxytyrosol (HT) a naturally occurring polyphenolic compound isolated from forsythia has excellent anti-inflammatory properties. However, effect mechanisms HT in ALI remain unclear. We used LPS/D-GalN induced experimental mouse model AML12 cells reveal efficacy potential ALI, HE staining was for evaluation pathologies. A biochemical assay detect changes function, RNA-seq conducted underlying WB, RT-qPCR, IF were assess effects action. Furthermore, vitro against protection mechanism. significant alleviated LPS/D-GalN-induced mice suppressing inflammatory. In terms RNA-seq, improved TNF, ECM-receptor interaction, PI3K/AKT signaling pathway, it downregulated mRNA levels VCAM-1, CXCL5, TNF-α IL-6 liver. Mechanically, targeting TNF-α, thereby inhibiting TNF-α/PI3K/AKT pathway.

Язык: Английский

Plantaginis Herba attenuates adriamycin-induced nephropathy: Molecular mechanism insights by integrated transcriptomic and experimental validation DOI
Beibei Zhang, Mengnan Zeng, Ru Wang

и другие.

Journal of Ethnopharmacology, Год журнала: 2025, Номер 341, С. 119331 - 119331

Опубликована: Янв. 6, 2025

Язык: Английский

Процитировано

0
Proteomics and phosphoproteomics analysis of acute pancreatitis alleviated by forsythoside B DOI Creative Commons
Linxiao Sun, Hongmei Li, Haiyan Zhang

и другие.

Journal of Proteomics, Год журнала: 2025, Номер 315, С. 105414 - 105414

Опубликована: Фев. 25, 2025

Язык: Английский

Процитировано

0
Artificial intelligence accelerates the identification of nature-derived potent LOXL2 inhibitors DOI Creative Commons
Xiaowei Jia, Meng Liu,

Yushi Tang

и другие.

Scientific Reports, Год журнала: 2025, Номер 15(1)

Опубликована: Март 27, 2025

Abstract The role of LOXL2 in cancer has been widely demonstrated, but current therapies targeting are not yet fully developed. We believe that selective nature-derived inhibition may provide a better therapeutic approach for the treatment cancer. Therefore, we adopted comprehensive combining deep learning and traditional computer-aided drug design methods to screen inhibitors. Bioactivity affinity potential inhibitors were determined by molecular docking virtual screening. At same time, experimentally tested effect on cells. Validation showed it could inhibit proliferation migration, promote apoptosis CT26 cells, reduce expression level protein. As result, identified potent inhibitor: natural product Forsythoside A, demonstrated A an inhibitory tumors.

Язык: Английский

Процитировано

0
Polyphyllin VI Ameliorates Pulmonary Fibrosis by Suppressing the MAPK/ERK and PI3K/AKT Signaling Pathways via Upregulating DUSP6 DOI

Yuting Xie,

Cailing Gan,

Hongyao Liu

и другие.

Phytotherapy Research, Год журнала: 2024, Номер 38(12), С. 5930 - 5948

Опубликована: Окт. 17, 2024

ABSTRACT Pulmonary fibrosis (PF) is a lethal disease caused by inordinate repair of damaged lungs, for which limited strategies are available. Polyphyllin VI (PPVI), extracted and isolated from Paris polyphylla Smith var. chinensis (Franch.) Hara, has been regarded as an important traditional Chinese herbal medicine the treatment respiratory system diseases. This study evaluated effects PPVI on PF its underlying mechanism. Experimental procedure For evaluating anti‐PF effect PPVI, we established in vivo mouse model via intratracheal infusion bleomycin (BLM) mice vitro induced TGF‐β1 NIH/3T3, HPF A549, respectively. Subsequently, mechanism was further explored using RNA sequencing (RNA‐Seq). The results demonstrated that significantly inhibited inflammation, oxidative damage, epithelial‐mesenchymal transition. Furthermore, indicated ameliorated modulating inflammation stress responses. dual specificity phosphatase 6 (DUSP6), shared most significant differentially expressed gene associated with response after treatment. Mechanistically, silencing DUSP6 can eliminate suppressive impact activation fibroblast phosphorylation ERK AKT. Summarily, our findings revealed potential mitigating upregulating highlighted regulatory function pathogenesis PF.

Язык: Английский

Процитировано

1
Hydroxytyrosol Alleviates Acute Liver Injury by Inhibiting the TNF-α/PI3K/AKT Signaling Pathway via Targeting TNF-α Signaling DOI Open Access

Zhining Gao,

Hui Dai, Qinqin Zhang

и другие.

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(23), С. 12844 - 12844

Опубликована: Ноя. 29, 2024

Acute liver injury (ALI) is an to tissue caused by viruses, drugs, alcohol, and oxygen deprivation, one of the most common serious clinical disorders. Hydroxytyrosol (HT) a naturally occurring polyphenolic compound isolated from forsythia has excellent anti-inflammatory properties. However, effect mechanisms HT in ALI remain unclear. We used LPS/D-GalN induced experimental mouse model AML12 cells reveal efficacy potential ALI, HE staining was for evaluation pathologies. A biochemical assay detect changes function, RNA-seq conducted underlying WB, RT-qPCR, IF were assess effects action. Furthermore, vitro against protection mechanism. significant alleviated LPS/D-GalN-induced mice suppressing inflammatory. In terms RNA-seq, improved TNF, ECM-receptor interaction, PI3K/AKT signaling pathway, it downregulated mRNA levels VCAM-1, CXCL5, TNF-α IL-6 liver. Mechanically, targeting TNF-α, thereby inhibiting TNF-α/PI3K/AKT pathway.

Язык: Английский

Процитировано

1