Macrophages in graft-versus-host disease (GVHD): dual roles as therapeutic tools and targets

Clinical and Experimental Medicine, Год журнала: 2025, Номер 25(1)

Опубликована: Март 6, 2025

Graft-versus-host disease remains one of the most formidable barriers to complete success hematopoietic stem cell transplantation that has emerged as curative approach for many malignancies because it affects quality life and overall survival. Macrophages are among important members immune system, which perform dual roles in GVHD both therapeutic tools targets. This review epitomizes multifunctional role macrophages pathophysiology acute chronic GVHD. play an early phase their recruitment infiltration into target organs. Furthermore, they polarize two functionally different phenotypes, including M1 M2. In case GVHD, express phenotype characterized by production pro-inflammatory cytokines contribute tissue damage. contrast, tend toward M2 associated with repair tissues fibrosis. A critical balance these phenotypes is central course severity Further interactions other lymphocytes such T cells, B fibroblast further determine Macrophage interaction alloreactive cells promotes inflammation. therefore inducing injuries during Interaction macrophages, cell, fibroblast, CD4+ fibrosis and, hence, subsequent dysfunction These some insights, while several challenges remain. First, impact dominant on polarization incompletely sometimes controversial. Second, development targeted therapies able modulate macrophage function without systemic side effects area ongoing investigation. Future directions involve exploration macrophage-targeted therapies, small molecules, antibodies, nanotechnology, behavior improve patient outcomes. underlines fact a profound understanding essential developing new more effective strategies. Targeting might represent avenue decreasing incidence improving safety HSCT.

Язык: Английский

---

Integrating single-cell sequencing and machine learning to uncover the role of mitophagy in subtyping and prognosis of esophageal cancer

APOPTOSIS, Год журнала: 2025, Номер unknown

Опубликована: Фев. 13, 2025

Язык: Английский

---

Emodin Decreases Tumor-Associated Macrophages Accumulation and Suppresses Bladder Cancer Development by Inhibiting CXCL1 Secretion from Cancer-Associated Fibroblasts

Nutrition and Cancer, Год журнала: 2025, Номер unknown, С. 1 - 16

Опубликована: Март 20, 2025

Tumor-associated macrophages (TAMs) and cancer-associated fibroblasts (CAFs) are the most abundant stromal cells in bladder cancer (BC) microenvironment (TME). However, detailed mechanisms underlying TAM-CAF communication their contributions to BC progression remain incompletely understood. Emerging evidence shows that Emodin exerts anti-tumor effect on several tumor models by targeting TME. To date, impact of has not been previously reported. Our study firstly demonstrated significantly inhibited growth reduced TAM accumulation a murine model. markedly decreased serum levels multiple chemokines tumor-bearing mice, with CXCL1 showing pronounced reduction. Strikingly, selectively suppressed secretion CAFs but TAMs or cells. Furthermore, decrease migration induced was dependent CAF-derived CXCL1. Using subcutaneous model, we found failed inhibit when CXCL1-deficient were co-injected cells, underscoring critical role this process. Bioinformatics analysis further revealed elevated correlated negatively invasive/metastatic potential overall survival patients. In conclusion, our findings establish delays disrupting CXCL1-mediated crosstalk between TAMs.

Язык: Английский

---

Integrating Single-Cell Sequencing and Machine Learning to Uncover the Role of Mitophagy in Subtyping and Prognosis of Esophageal Cancer

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Окт. 14, 2024

Globally, esophageal cancer stands as a prominent contributor to cancer-related fatalities, distinguished by its grim prognosis. Mitophagy has significant impact on the process of progression. This study investigates prognostic significance mitophagy-related genes (MRGs) in carcinoma (ESCA) with aim elucidating molecular subtypes. By analyzing RNA-seq data from The Cancer Genome Atlas (TCGA), 6451 differentially expressed (DEGs) were identified. Cox regression analysis narrowed this list 14 MRGs notable implications. ESCA patients classified into two distinct subtypes (C1 and C2) based these genes. Furthermore, leveraging between Cluster 1 2, novel (CA CB). Notably, C2 CA exhibited inferior prognosis compared those C1 CB (p < 0.05). Functional enrichments immune microenvironments varied significantly among subtypes, demonstrating higher checkpoint expression levels. Employing machine learning algorithms like LASSO Random Forest, alongside multivariate COX analysis, core genes: HSPD1 MAP1LC3B A robust model was developed validated external cohorts. Additionally, single-cell sequencing provided insights microenvironment heterogeneity. Through Coremine database screening, Icaritin emerged potential therapeutic candidate improve Molecular docking results indicated favorable binding efficacies MAP1LC3B, enhancing comprehension underlying mechanisms offering avenues.

Язык: Английский

---

Engineered Cu-VT composite nanoparticles induce copper-dependent cell death in bladder cancer: insights from single-cell spatial transcriptomics

Advanced Composites and Hybrid Materials, Год журнала: 2024, Номер 8(1)

Опубликована: Дек. 24, 2024

Язык: Английский

---